Allele Dmel\Nedd4^{ΔMid.Scer\UAS.Long.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Nedd4^{ΔMid.Scer\UAS.Long.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0265781
Feature type	allele	Associated gene	Dmel\Nedd4

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Zhong et al., 2011) in vitro construct - regulatory fusion (Zhong et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the long isoform of Nedd4 lacking the middle region (bp 912 to 1419) of Nedd4 and fused to an N-terminal T:Zzzz\FLAG tag. (Zhong et al., 2011)
Carried in construct	P{UAS-Nedd4.Lo.FLAG.ΔMid} (Zhong et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| third instar larval stage, with Scer\GAL4⁰⁰⁵ (Zhong et al., 2011)
Phenotype Manifest In
	intersegmental nerve branch ISNb of A1-7, with Scer\GAL4⁰⁰⁵ (Zhong et al., 2011)
Detailed Description
	Statement Reference Flies overexpressing Nedd4[ΔMid.Scer\UAS.Long.T:Zzzz\FLAG] ubiquitously driven by any one of Scer\GAL4[Act5C.PI], Scer\GAL4[da.G32] or Scer\GAL4[αTub84B.PL] survive to the adult stage. Muscle specific overexpression of Nedd4[ΔMid.Scer\UAS.Long.T:Zzzz\FLAG] driven by muscle drivers Scer\GAL4[how-24B] or Scer\GAL4[005] does not result in any lethality. Larvae overexpressing Nedd4[ΔMid.Scer\UAS.Long.T:Zzzz\FLAG] under the control of Scer\GAL4[005] exhibit muscle innervation abnormalities compared with wild-type. (Zhong et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Nedd4^{ΔMid.Scer\UAS.Long.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Zhong et al., 2011, PLoS ONE 6(11): e27007 A Splice Isoform of DNedd4, DNedd4-Long, Negatively Regulates Neuromuscular Synaptogenesis and Viability in Drosophila. [FBrf0216790]

Allele Dmel\Nedd4ΔMid.Scer\UAS.Long.T:Zzzz\FLAG

Allele Dmel\Nedd4^{ΔMid.Scer\UAS.Long.T:Zzzz\FLAG}