Allele Dmel\ckn^K.Δ324-331

General Information
Symbol	Dmel\ckn^K.Δ324-331	Species	D. melanogaster
Name		FlyBase ID	FBal0265796
Feature type	allele	Associated gene	Dmel\ckn

Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Weng et al., 2011)
Mutagen	ethyl methanesulfonate (Weng et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{GSV1}GS1205 (Weng et al., 2011)
Nature of the lesion	Statement Reference Treatment with 22mM ethyl methanesulfonate generates a deletion removing residues 324-331, but does not result in a frameshift. (Weng et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| embryonic stage (Weng et al., 2011) neuroanatomy defective \| embryonic stage (with Df(2R)BSC330) (Weng et al., 2011)
Phenotype Manifest In
	intersegmental nerve \| embryonic stage (Weng et al., 2011) intersegmental nerve \| embryonic stage (with Df(2R)BSC330) (Weng et al., 2011) intersegmental nerve branch ISNb of A1-7 \| embryonic stage (Weng et al., 2011) intersegmental nerve branch ISNb of A1-7 \| embryonic stage (with Df(2R)BSC330) (Weng et al., 2011)
Detailed Description
	Statement Reference ckn[K.Δ324-331] homozygous or ckn[K.Δ324-331]/Df(2R)BSC330 mutant embryos display consistent motor axon projection defects, exhibiting classic ISNb 'bypass' phenotypes, where ISNb axons fail to innervate the ventrolateral muscle field. These axons appear to defasciculate normally from the primary ISN branch at the exit junction, but fail to enter the ventral muscle field, instead bypassing their targets as they extend parallel to the primary ISN fascicle. Frequently, mis-targeted ISNb axons reach back from the dorsal edge of muscle 12 to innervate the ventrolateral muscle field. In the majority of affected nerves, ISNb axons are visible as a distinct fascicle next to the ISN. ckn[K.Δ324-331] heterozygous embryos do not exhibit ISNb guidance defects. (Weng et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference ckn^K.Δ324-331 has neuroanatomy defective \| embryonic stage phenotype, non-enhanceable by Ptp69D[+]/Ptp69D⁷ (Weng et al., 2011) ckn^K.Δ324-331 has neuroanatomy defective \| embryonic stage phenotype, non-enhanceable by Ptp69D¹/Ptp69D[+] (Weng et al., 2011)
NOT suppressed by
Statement Reference ckn^K.Δ324-331 has neuroanatomy defective \| embryonic stage phenotype, non-suppressible by Ptp69D[+]/Ptp69D⁷ (Weng et al., 2011) ckn^K.Δ324-331 has neuroanatomy defective \| embryonic stage phenotype, non-suppressible by Ptp69D¹/Ptp69D[+] (Weng et al., 2011)
Enhancer of
Statement Reference ckn^K.Δ324-331 is an enhancer of neuroanatomy defective \| embryonic stage phenotype of Lar^5.5 (Weng et al., 2011)
Other
Statement Reference ckn^K.Δ324-331, dock³ has neuroanatomy defective \| embryonic stage phenotype (Weng et al., 2011)
Phenotype Manifest In
NOT Enhanced by
Statement Reference ckn^K.Δ324-331 has intersegmental nerve \| embryonic stage phenotype, non-enhanceable by Ptp69D[+]/Ptp69D⁷ (Weng et al., 2011) ckn^K.Δ324-331 has intersegmental nerve \| embryonic stage phenotype, non-enhanceable by Ptp69D¹/Ptp69D[+] (Weng et al., 2011) ckn^K.Δ324-331 has intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype, non-enhanceable by Ptp69D[+]/Ptp69D⁷ (Weng et al., 2011) ckn^K.Δ324-331 has intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype, non-enhanceable by Ptp69D¹/Ptp69D[+] (Weng et al., 2011)
NOT suppressed by
Statement Reference ckn^K.Δ324-331 has intersegmental nerve \| embryonic stage phenotype, non-suppressible by Ptp69D[+]/Ptp69D⁷ (Weng et al., 2011) ckn^K.Δ324-331 has intersegmental nerve \| embryonic stage phenotype, non-suppressible by Ptp69D¹/Ptp69D[+] (Weng et al., 2011) ckn^K.Δ324-331 has intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype, non-suppressible by Ptp69D[+]/Ptp69D⁷ (Weng et al., 2011) ckn^K.Δ324-331 has intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype, non-suppressible by Ptp69D¹/Ptp69D[+] (Weng et al., 2011)
Enhancer of
Statement Reference ckn^K.Δ324-331/ckn[+] is an enhancer of intersegmental nerve branch ISNb of A1-7 phenotype of dock³ (Weng et al., 2011) ckn^K.Δ324-331 is an enhancer of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of Lar^5.5 (Weng et al., 2011) ckn^K.Δ324-331 is an enhancer of intersegmental nerve branch ISNb of A1-7 phenotype of dock³ (Weng et al., 2011)
Other
Statement Reference ckn^K.Δ324-331, dock³ has intermediate longitudinal fascicle \| embryonic stage phenotype (Weng et al., 2011) ckn^K.Δ324-331, dock³ has intersegmental nerve branch ISNd of A1-7 phenotype (Weng et al., 2011) ckn^K.Δ324-331, dock³ has lateral longitudinal fascicle \| embryonic stage phenotype (Weng et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Lar[5.5] ckn[K.Δ324-331] double heterozygous embryos display bypass phenotypes with 39% penetrance. A heterozygous or homozygous Ptp69D[1] background fails to affect the penetrance of the ISNb bypass phenotype found in ckn[K.Δ324-331] heterozygotes or homozygotes. A heterozygous or homozygous Ptp69D[7] background fails to affect the penetrance of the ISNb bypass phenotype found in ckn[K.Δ324-331] heterozygotes or homozygotes. ISNb pathfinding is delayed in approximately 23% of dock[3] ckn[K.Δ324-331] double heterozygotes. A heterozygous ckn[K.Δ324-331] background enhances ISNb pathfinding defects in dock[3] homozygotes, from 6% to 43% of hemisegments. dock[3] ckn[K.Δ324-331] double homozygotes exhibit delayed 'immature' ISNb axons in 65% of hemisegments. Motor axons in the affected nerves are loosely organised with multiple projections and resemble wild-type axons at earlier stages. Furthermore, the ISNd branch is frequently absent or reduced in size. Examination of the ISNb/d choice point reveals defects in ISNb/d branch segregation. The lateral two longitudinal Fas2-positive fascicles are poorly fasciculated and discontinuous in these double mutants. (Weng et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	ckn^K.Δ324-331/Scer\GAL4^elav.PLu rescues ckn^Scer\UAS.cWa (Weng et al., 2011)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ckn^K.Δ324-331 ckn^K (Weng et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Weng et al., 2011, J. Neurosci. 31(12): 4421--4433 The Cytoplasmic Adaptor Protein Caskin Mediates Lar Signal Transduction during Drosophila Motor Axon Guidance. [FBrf0213321]

Allele Dmel\cknK.Δ324-331

Allele Dmel\ckn^K.Δ324-331