FlyBase Allele Report: Dmel\caup[UAS.Tag:HA]

General Information

Symbol

Dmel\caup^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0265897

Feature type

allele

Associated gene

Dmel\caup

Associated Insertion(s)

Carried in Construct

M{UAS-caup.HA}

Key Links

Transgenic product class

wild_type

(Carrasco-Rando et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Carrasco-Rando et al., 2011)

Mutagen

in vitro construct

(Carrasco-Rando et al., 2011)

Progenitor genotype

Carried in construct

M{UAS-caup.HA}

Cytology

Description

UAS regulatory sequences drive expression of caup which is tagged with a Tag:HA epitope.

(Carrasco-Rando et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

caup

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage, with Scer\GAL4^ap-md544

(Barrios et al., 2015)

decreased occurrence of cell division | larval stage, with Scer\GAL4^nub.PU

(Barrios et al., 2015)

increased cell death, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

visible, with Scer\GAL4^ey.PU

(Barrios et al., 2015)

visible, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

wing disc, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

wing disc | larval stage, with Scer\GAL4^ap-md544

(Barrios et al., 2015)

wing disc | larval stage, with Scer\GAL4^nub.PU

(Barrios et al., 2015)

wing vein, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Detailed Description

Statement

Reference

Expression of caup^{Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^nub.PU or Scer\GAL4^ap-md544 (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80^ts.αTub84B) causes a significant reduction in the mitotic index in the wing disc. Cell size is not noticeably affected.

Expression of caup^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^salm.EPv results in a reduction in the size of the central wing pouch of the wing disc and the adult wings. The adult wings show an altered venation pattern and wing margin notches. Enlarged cells are also seen in the Scer\GAL4^salm.EPv-expressing domain. Apoptosis is increased compared with controls, especially in the central part of the Scer\GAL4^salm.EPv-expressing domain. Reducing apoptosis using one copy of Df(3L)H99 (which removes the cell-death-inducing genes (rpr, hid and grim) does not modify the wing phenotypes.

(Barrios et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has visible phenotype, enhanceable by dap^UAS.cUa/Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has visible phenotype, enhanceable by Scer\GAL4^salm.EPv/ago^HM04005

(Barrios et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has increased cell death phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^nub.PU, caup^UAS.Tag:HA has decreased occurrence of cell division | larval stage phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^nub.PU

(Barrios et al., 2015)

Scer\GAL4^ap-md544, caup^UAS.Tag:HA has decreased occurrence of cell division | larval stage phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ap-md544

(Barrios et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has visible phenotype, non-suppressible by Diap1^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has visible phenotype, non-suppressible by Scer\GAL4^salm.EPv/stg^UAS.cNa

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has visible phenotype, non-suppressible by Cdk2^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing phenotype, enhanceable by dap^UAS.cUa/Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing vein phenotype, enhanceable by dap^UAS.cUa/Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing phenotype, enhanceable by Scer\GAL4^salm.EPv/ago^HM04005

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing vein phenotype, enhanceable by Scer\GAL4^salm.EPv/ago^HM04005

(Barrios et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing vein phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^nub.PU, caup^UAS.Tag:HA has wing disc | larval stage phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^nub.PU

(Barrios et al., 2015)

Scer\GAL4^ap-md544, caup^UAS.Tag:HA has wing disc | larval stage phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ap-md544

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing disc phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing phenotype, non-suppressible by Diap1^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing vein phenotype, non-suppressible by Diap1^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing phenotype, non-suppressible by Scer\GAL4^salm.EPv/stg^UAS.cNa

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing phenotype, non-suppressible by Cdk2^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Scer\GAL4^salm.EPv, caup^UAS.Tag:HA has wing vein phenotype, non-suppressible by Cdk2^UAS.cUa, Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^salm.EPv/caup^UAS.Tag:HA is a suppressor of eye phenotype of Scer\GAL4^salm.EPv, yki^UAS.cUa

(Barrios et al., 2015)

Other

Statement

Reference

CycE^UAS.cLa, Scer\GAL4^salm.EPv, caup^UAS.Tag:HA, yki^UAS.cUa has eye phenotype

(Barrios et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Diap1^Scer\UAS.cUa suppresses the apoptosis seen in the wing disc when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv. The wing size, vein pattern and wing notching phenotypes are not modified by Diap1^Scer\UAS.cUa.

Expression of stg^Scer\UAS.cNa does not suppress the reduction in wing size and cell number seen when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

Expression of CycE^Scer\UAS.cLa suppresses the wing phenotypes seen when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

Expression of CycE^Scer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of either Scer\GAL4^nub.PU or Scer\GAL4^ap-md544 (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80^ts.αTub84B).

Expression of Cdk2^Scer\UAS.cUa does not suppress the wing phenotypes seen when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

Expression of dap^Scer\UAS.cUa enhances the wing phenotypes seen when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

Expression of ago^HM04005 enhances the wing phenotypes seen when caup^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

Expression of caup^{Scer\UAS.T:Ivir\HA1} suppresses the eye disc overgrowth seen when yki^Scer\UAS.cUa is expressed under the control of Scer\GAL4^salm.EPv. This suppression is partially reverted upon co-expression of CycE^Scer\UAS.cLa.

(Barrios et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

caup^{Scer\UAS.T:Ivir\HA1}

caup^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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