Allele Dmel\gek^{ΔPH.Scer\UAS}

General Information
Symbol	Dmel\gek^{ΔPH.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0265917
Feature type	allele	Associated gene	Dmel\gek

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Gontang et al., 2011) in vitro construct - regulatory fusion (Gontang et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS sequences drive expression of a mutant form of gek lacking the plekstrin homology domain. (Gontang et al., 2011)
Carried in construct	P{UAS-gek.ΔPH} (Gontang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| third instar larval stage, with gek^omb1080/Df(2R)e03819-f02302, Scer\GAL4^Glass38-1 (Gontang et al., 2011) visible \| adult stage, with Scer\GAL4^Glass38-1 (Gontang et al., 2011)
Phenotype Manifest In
	embryonic/larval optic stalk \| third instar larval stage, with gek^omb1080/Df(2R)e03819-f02302, Scer\GAL4^Glass38-1 (Gontang et al., 2011) eye, with Scer\GAL4^Glass38-1 (Gontang et al., 2011) eye photoreceptor cell \| third instar larval stage, with gek^omb1080/Df(2R)e03819-f02302, Scer\GAL4^Glass38-1 (Gontang et al., 2011) lamina \| third instar larval stage, with gek^omb1080/Df(2R)e03819-f02302, Scer\GAL4^Glass38-1 (Gontang et al., 2011)
Detailed Description
	Statement Reference Expression of gek[ΔPH.Scer\UAS] under the control of Scer\GAL4[Glass38-1] results in normal R cell axon targeting in third instar larvae. No photoreceptor morphology defects are seen in the eye discs, but adult animals have rough eyes. Expression of gek[ΔPH.Scer\UAS] under the control of Scer\GAL4[Glass38-1] in gek[omb1080]/Df(2R)e03819-f02302 mutant third instar larvae results in early termination of many R cell growth cones, with large clumps appearing in the optic stalk and the distal lamina. (Gontang et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Scer\GAL4^elav-C155/gek^{ΔPH.Scer\UAS} partially rescues gek^omb1080 (Gontang et al., 2011)
Comments	Expression of gek[ΔPH.Scer\UAS] in pupal stage R cells under the control of Scer\GAL4[elav-C155] rescues the axon targeting phenotypes seen in gek[omb1080] homozygous clones. 83% of axons extend correctly, 12% mistarget and 5% do not extend. Premature stopping of R1-R6 axons above the lamina plexus is not observed. However, approximately 18% of R cells exhibit morphological defects, with apical domains extending too far towards the center of the ommatidium or being located basally. 6% of all R cell bodies are below the plane of the ommatidium. All remaining R cells appear normal. (Gontang et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	gek^{ΔPH.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Gontang et al., 2011, Development 138(22): 4899--4909 The cytoskeletal regulator Genghis khan is required for columnar target specificity in the Drosophila visual system. [FBrf0216508]

Allele Dmel\gekΔPH.Scer\UAS

Allele Dmel\gek^{ΔPH.Scer\UAS}