FB2025_01 , released February 20, 2025
Allele: Scer\GAL4twi.PU
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General Information
Symbol
Scer\GAL4twi.PU
Species
S. cerevisiae
Name
FlyBase ID
FBal0265922
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{twi-GAL4.U}
Also Known As
twi-Gal4, twist-Gal4, twi::Gal4, twiGal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(FlyBase, 1992-)
Progenitor genotype
Carried in construct
P{twi-GAL4.U}
Cytology
Description

twi sequences regulate expression of a GAL4 driver.

(FlyBase, 1992-)
Allele components
Component
Use(s)
Regulatory region(s)
twi
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 12
      ectoderm
      (Hafer et al., 2011)
      cell of mesoderm
      • nucleus
      (Hafer et al., 2011)
      cell of mesoderm
      (Hafer et al., 2011)
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 17
      embryonic heart ostial cardioblast
      (Wu and Sato, 2008)
      Additional Information
      Statement
      Reference
       
      Marker for
      embryonic heart ostial cardioblast
      (Wu and Sato, 2008)
      Reflects expression of
      twi
      (Hafer et al., 2011, Wu and Sato, 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal 1 lateral transverse muscle cell | embryonic stage, with AmphHMJ21356

      (Collins et al., 2017)

      abdominal 1 lateral transverse muscle cell | embryonic stage, with klarHMS01612

      (Collins et al., 2017)

      border follicle cell | adult stage, with α-Cat1, α-CatST-D.UASp.Tag:HA

      (Escobar et al., 2015)

      border follicle cell | adult stage, with α-CatΔP.UASp.Tag:HA, α-Cat1

      (Escobar et al., 2015)

      embryo, with Cdc42N17.UAS

      (Clark et al., 2011)

      embryonic/larval tracheal section, with stumps802.Y515F.UAS.Tag:FLAG, stumpsunspecified

      (Csiszar et al., 2010)

      embryonic/larval tracheal section, with stumps802.Y515F.Y613F.Y629F.UAS.Tag:FLAG, stumpsunspecified

      (Csiszar et al., 2010)

      embryonic/larval tracheal section, with stumpsY515F.Y613F.Y629F.UAS.Tag:FLAG, stumpsunspecified

      (Csiszar et al., 2010)

      muscle cell of ventral longitudinal muscle 3 | larval stage, with AmphHMJ21356

      (Collins et al., 2017)

      muscle cell of ventral longitudinal muscle 3 | larval stage, with bocksHMS01817

      (Collins et al., 2017)

      muscle cell of ventral longitudinal muscle 3 | larval stage, with klarHMS01612

      (Collins et al., 2017)

      muscle cell of ventral longitudinal muscle 3 | larval stage, with koiHMS02172

      (Collins et al., 2017)

      muscle cell of ventral longitudinal muscle 3 | larval stage, with mtmJF01114

      (Collins et al., 2017)

      muscle cell of ventral longitudinal muscle 3 | larval stage, with OteHMS01926

      (Collins et al., 2017)

      nucleus, with AmphHMJ21356

      (Collins et al., 2017)

      nucleus, with bocksHMS01817

      (Collins et al., 2017)

      nucleus, with klarHMS01612

      (Collins et al., 2017)

      nucleus, with koiHMS02172

      (Collins et al., 2017)

      nucleus, with mtmJF01114

      (Collins et al., 2017)

      nucleus, with OteHMS01926

      (Collins et al., 2017)

      pericardial cell primordium, with stumps802.Y515F.UAS.Tag:FLAG, stumpsunspecified

      (Csiszar et al., 2010)

      pericardial cell primordium, with stumps802.Y515F.Y613F.Y629F.UAS.Tag:FLAG, stumpsunspecified

      (Csiszar et al., 2010)

      pericardial cell primordium, with stumpsY515F.Y613F.Y629F.UAS.Tag:FLAG, stumpsunspecified

      (Csiszar et al., 2010)

      visceral muscle fiber | embryonic stage, with mskUAS.cLa

      (Liu et al., 2013)
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4twi.PU, mskUAS.cLa has visceral muscle fiber | embryonic stage phenotype, suppressible by mbcD11.2/mbc[+]

      (Liu et al., 2013)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4twi.PU/htlλ.UAS is a non-suppressor of mesoderm | germline clone phenotype of nst16923

      (Mariappa et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4twi.PU
      Name Synonyms
      Secondary FlyBase IDs
        References (39)