FlyBase Allele Report: Hsap\ALK[UAS.cSa]

General Information

Symbol

Hsap\ALK^UAS.cSa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Schonherr

FlyBase ID

FBal0266009

Feature type

allele

Associated gene

Hsap\ALK

Associated Insertion(s)

Carried in Construct

P{UAS-hALK.S}

Also Known As

UAS-ALK^wt, UAS-ALK, UAS-hALK

Key Links

Transgenic product class

wild_type

(Schönherr et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Schönherr et al., 2011)

Mutagen

in vitro construct

(Schönherr et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-hALK.S}

Cytology

Description

UAS regulatory sequences drive expression of a wild-type Hsap\ALK. An 898bp fragment preceeding the translation start site has been removed to increase expression efficiency in Drosophila.

(Schönherr et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\ALK

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Hsap\ALKAL1^UAS.cGa

(Guan et al., 2015)

CEA with Hsap\ALKAL2^UAS.cGa

(Guan et al., 2015)

model of neuroblastoma

CEA with Hsap\ALKAL1^UAS.cGa

(Wolfstetter et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing Hsap\ALK^UAS.cSa under the control of Scer\GAL4^GMR.PF has no impact on eye phenotype.

(Fadeev et al., 2018)

The expression of Hsap\ALK^UAS.cSa under the control of Scer\GAL4^GMR.PU does not induce an obvious eye phenotype.

(Guan et al., 2017)

Expression of Hsap\ALK^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF does not induce any obvious eye morphological phenotype in adult flies.

(Guan et al., 2017)

Expression of Hsap\ALK^Scer\UAS.cSa driven by Scer\GAL4^GMR.PFa does not result in significant eye phenotypes compared to controls.

(Siaw et al., 2016)

Scer\GAL4^GMR.PF-mediated expression has no effect on adult eye morphology.

(Hugosson et al., 2014)

Expression of Hsap\ALK^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PFa does not result in any obvious mutant phenotype in the adult eye.

(Chand et al., 2013)

Expression of Hsap\ALK^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PFa does not lead to any obvious morphological defects in the adult eye.

(Martinsson et al., 2011)

Expression of Hsap\ALK^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PFa does not result in any obvious defect in the adult eye.

(Schönherr et al., 2011)

Expression of Hsap\ALK^Scer\UAS.cSa in the developing eye, under the control of Scer\GAL4^GMR.PFa, does not result in any obvious phenotype.

(Schönherr et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Hsap\ALK^UAS.cSa, Hsap\ALKAL1^UAS.cGa, Scer\GAL4^GMR.PF has visible | adult stage phenotype

(Fadeev et al., 2018, Guan et al., 2017)

Hsap\ALK^UAS.cSa, Hsap\ALKAL2^UAS.cGa, Scer\GAL4^GMR.PF has visible | adult stage phenotype

(Fadeev et al., 2018, Guan et al., 2017)

Hsap\ALK^UAS.cSa, Hsap\ALKAL1^UAS.cGa, Scer\GAL4^GMR.PF has some die during pupal stage phenotype

(Guan et al., 2017)

Phenotype Manifest In

Other

Statement

Reference

Hsap\ALK^UAS.cSa, Hsap\ALKAL1^UAS.cGa, Scer\GAL4^GMR.PF has eye phenotype

(Fadeev et al., 2018, Guan et al., 2017)

Hsap\ALK^UAS.cSa, Hsap\ALKAL2^UAS.cGa, Scer\GAL4^GMR.PF has eye phenotype

(Fadeev et al., 2018, Guan et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing Hsap\ALK^UAS.cSa and either Hsap\ALKAL1^UAS.cGa or Hsap\ALKAL2^UAS.cGa under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Fadeev et al., 2018)

Xenogenetic Interactions

Statement

Reference

Simultaneous expression of Hsap\ALK^Scer\UAS.cSa and Hsap\ALKAL1^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PF leads to a severe eye degeneration phenotype in adult flies and increased pupal lethality, co-expression of Hsap\ALK^Scer\UAS.cSa and Hsap\ALKAL2^Scer\UAS.cGa results in strong rough eye phenotype.

(Guan et al., 2017)

Scer\GAL4^GMR.PF-mediated co-expression of Hsap\ALK^Scer\UAS.cSa and Hsap\MDK^Scer\UAS.cHa, or Hsap\ALK^Scer\UAS.cSa and Hsap\PTN^Scer\UAS.cHa, has no effect on adult eye morphology.

(Hugosson et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ALK^wt

(Chand et al., 2013)

Hsap\ALK^Scer\UAS.cSa

Hsap\ALK^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schonherr

Secondary FlyBase IDs

References (12)

Report Sections

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