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General Information
Symbol
Dmel\Cul3NIG.11861R
Species
D. melanogaster
Name
FlyBase ID
FBal0266018
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
11861R
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a Cul3 RNAi construct.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A limited number of adult flies can be obtained when Cul-3NIG.11861R is expressed under the control of Scer\GAL4Clk.8424.

Under light-dark conditions, flies expressing Cul-3NIG.11861R under the control of either Scer\GAL4P2.4.Pdf or Scer\GAL4Clk.8424 show no or reduced morning anticipatory activity. After transfer to constant darkness conditions, the flies either show weak locomotor activity rhythms or become arrhythmic.

Flies expressing Cul-3NIG.11861R under the control of Scer\GAL4P2.4.Pdf which are raised at 25[o]C and tested at 20[o]C show weaker locomotor activity rhythm defects under constant darkness conditions than flies raised and tested at 25[o]C, indicating that Cul-3 function is required during the adult stage for normal locomotor activity rhythms.

Flies expressing Cul-3NIG.11861R under the control of Scer\GAL41118 show reduced morning anticipation under light-dark conditions. Under constant darkness conditions, only 44% of the flies are rhythmic for locomotor activity and the rhythmic flies display weak rhythms.

Under constant darkness conditions, only 30% of flies expressing Cul-3NIG.11861R under the control of Scer\GAL4cry.PE are rhythmic for locomotor activity and the rhythmic flies display weak rhythms.

Expression of Cul-3NIG.11861R under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi effects) results in sleep defects: the flies show a reduction in sleep duration compared to controls, with a reduction in the average sleep bout length and an increase in latency to sleep after lights-off. The mutant flies show defects in sleep homeostasis, lacking a detectable sleep rebound after sleep deprivation. The mutant flies are more easily woken by a rotational stimulus compared to wild type controls.

Expression of Cul-3NIG.11861R under the control of Scer\GAL430Y (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi effects) has no affect on sleep.

Expression of Cul-3NIG.11861R under the control of Scer\GAL4elav.Switch.PO during development (using RU486 to control the timing of expression and in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi effects) results in reduced sleep duration, while expression of incKK108908 under the control of Scer\GAL4elav.Switch.PO during the adult stage only does not result in sleep defects.

Expression of Cul-3NIG.11861R under the control of Scer\GAL4elav-C155 results in a small decrease in sleep duration. In the presence of Dcr-2Scer\UAS.cDa (to enhance the strength of RNAi) these animals show a severe decrease in sleep duration and bout length compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of both incKK108908 and Cul-3NIG.11861R under the control of Scer\GAL430Y (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi effects) results in a significant decrease in sleep duration and average sleep bout length.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Co-expression of Cul-3Scer\UAS.cMa partially rescues the mutant sleep phenotypes caused by expression of Cul-3NIG.11861R under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi effects).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Cul-3NIG.11861R
Cul3NIG.11861R
Name Synonyms
Secondary FlyBase IDs
    References (3)