Allele Dmel\mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0266181
Feature type	allele	Associated gene	Dmel\mbc

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion, in vitro construct - amino acid replacement, in vitro construct - deletion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Balagopalan et al., 2006) in vitro construct - coding region fusion (Balagopalan et al., 2006) in vitro construct - deletion (Balagopalan et al., 2006) in vitro construct - regulatory fusion (Balagopalan et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS drives expression of a mbc cDNA with a deletion of amino acids 1807 to 1970 (the entire C terminus), and in which the prolines in the N-terminal PXXP motif at amino acids 110-113 are converted to alanines, and tagged with T:Ivir\HA1. (Balagopalan et al., 2006)
Carried in construct	P{UAS-mbc.NPxxP.Δ1807.HA} (Balagopalan et al., 2006, Haralalka et al., 2011)
Tagged with	T:Ivir\HA1 (Balagopalan et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Scer\GAL4[twi.PG]-mediated expression of mbc[NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1] does not produce muscle phenotypes. (Balagopalan et al., 2006)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^sns.PK rescues mbc^D11.2 (Haralalka et al., 2011) Scer\GAL4^how-24B, mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^how-24B rescues mbc^D11.2/Df(3R)mbc⁹ (Balagopalan et al., 2006) Scer\GAL4^twi.PG/mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1} rescues mbc^D11.2 (Balagopalan et al., 2006)
Comments	Expression of mbc[NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[sns.PK] restores fusion in mbc[D11.2] embryos and a wild-type somatic muscle pattern is formed. (Haralalka et al., 2011) Scer\GAL4[twi.PG]- or Scer\GAL4[how-24B]-mediated expression of mbc[NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1] rescues the mbc[D11.2] myoblast fusion defect. (Balagopalan et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Haralalka et al., 2011, Development 138(8): 1551--1562 Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. [FBrf0213340] Balagopalan et al., 2006, Mol. Cell. Biol. 26(24): 9442--9455 The CDM superfamily protein MBC directs myoblast fusion through a mechanism that requires phosphatidylinositol 3,4,5-triphosphate binding but is independent of direct interaction with DCrk. [FBrf0194774]

Allele Dmel\mbcNPxxP.Δ1807.Scer\UAS.T:Ivir\HA1

Allele Dmel\mbc^{NPxxP.Δ1807.Scer\UAS.T:Ivir\HA1}