FlyBase Allele Report: Dmel\Cul3[HMS01572]

General Information

Symbol

Dmel\Cul3^HMS01572

Species

D. melanogaster

Name

FlyBase ID

FBal0266479

Feature type

allele

Associated gene

Dmel\Cul3

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01572}

Also Known As

HMS01572

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01572}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01572

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin cytoskeleton | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2015)

filamentous actin | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2019)

nurse cell ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2019, Hudson et al., 2015)

Detailed Description

Statement

Reference

Expressing Cul3^HMS01572 under the control of Scer\GAL4^{VP16.mat.αTub67C} induces a thicker F-actin cytoskeleton in egg chamber ring canals.

(Hudson et al., 2019)

Expression of Cul3^HMS01572 RNAi under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in a ring canal phenotype in the egg chambers: the F-actin cytoskeleton is disorganized, the kel protein accumulates in the lumen and the lumen diameter is significantly smaller compared to controls.

(Hudson et al., 2015)

Expression of Cul3^HMS01572 in somatic cyst cells under the control of any of Scer\GAL4^tj-NP1624, Scer\GAL4^A3.eya or Scer\GAL4^c784 (limited to a week from eclosion onwards using Scer\GAL80^ts.αTub84B) does not disrupt germline enclosure in testes, and cytoplasmic extensions from the cyst cells are clearly seen in the expected net-like pattern around the germline.

(Qian et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Cul3^HMS01572, Scer\GAL4^{VP16.mat.αTub67C} has nurse cell ring canal phenotype, suppressible by hts[+]/hts^T776fs

(Hudson et al., 2019)