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General Information
Symbol
Dmel\Sema2bUAS.GFP,Tag:M(mCd8a)
Species
D. melanogaster
Name
FlyBase ID
FBal0266711
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression Sema2b which is tagged at the C-terminus with the transmembrane region of Mmus\Cd8a and with GFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Sema2bUAS.GFP,Tag:M(mCd8a) under the control of Scer\GAL4GMR83H12 leads to attraction of P-Fr processes to the ventral fan-shaped body and dorsal noduli in the adult brain when compared to controls.

5.4% of hemisegments show defects in the SNa pathway (the SNa lateral pathway ectopically contacts muscle 12) in embryos expressing one copy of Sema-2bScer\UAS.T:Avic\GFP,T:Mmus\Cd8a under the control of Scer\GAL4tey-5053A. The penetrance of the phenotype increases to 28.2% of hemisegments when two copies of Sema-2bScer\UAS.T:Avic\GFP,T:Mmus\Cd8a are used.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The increased dendritic non-contacting crossing in the class IV da neurons seen in Sema-2bf02042/Df(2R)Sema-2b-C4 mutant larvae can be rescued by ectopic expression of Sema-2bScer\UAS.cUa or Sema-2bScer\UAS.T:Avic\GFP,T:Mmus\Cd8a under the Scer\GAL4A58 driver, whereas expression of Sema-2bScer\UAS.cUa under Scer\GAL4ppk.PU does not rescue the phenotype.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Sema-2bScer\UAS.T:Avic\GFP,T:Mmus\Cd8a
Sema2bScer\UAS.T:Avic\GFP,T:Mmus\Cd8a
Sema2bUAS.GFP,Tag:M(mCd8a)
Name Synonyms
Secondary FlyBase IDs
    References (5)