Allele Dmel\brp^5.45

General Information
Symbol	Dmel\brp^5.45	Species	D. melanogaster
Name		FlyBase ID	FBal0266720
Feature type	allele	Associated gene	Dmel\brp

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Fouquet et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:5,421,379..5,421,379 na_change=C5421379T pr_change=Q524 \| brp-PI; Q1365 \| brp-PJ; Q834 \| brp-PK; Q908 \| brp-PL; Q867 \| brp-PD; Q867 \| brp-PE; Q913 \| brp-PG; Q867 \| brp-PH; Q625@ \| brp-PM reported_pr_change=?867@ comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. (Fouquet et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: ?867@. (Fouquet et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Df(2R)BSC29) (Fouquet et al., 2009) locomotor rhythm defective (Hallermann et al., 2010) neuroanatomy defective \| third instar larval stage (Fouquet et al., 2009) neurophysiology defective (Hallermann et al., 2010) short lived (Hallermann et al., 2010)
Phenotype Manifest In
	embryonic/larval neuromuscular junction \| third instar larval stage (Fouquet et al., 2009) neuromuscular junction (Hallermann et al., 2010) presynaptic cytoskeletal matrix assembled at active zones \| third instar larval stage (Fouquet et al., 2009) synapse (Hallermann et al., 2010) synaptic vesicle (Hallermann et al., 2010)
Detailed Description
	Statement Reference brp[5.45] mutants exhibit significantly reduced survival rates and motor abilities. In brp[5.45] mutants, with poorly clustered Ca[2+] channels, the EPSC amplitudes are reduced to approximately 10% of control amplitudes, The amplitude of miniature EPSCs is not significantly affected. Depression decay during paired-pulse stimulation is stronger in brp[5.45] mutants, compared to controls. Consistent with the altered paired-pulse ratio, the amplitude of the second EPSC in the train is also significantly reduced. brp[5.45] mutant synapses display enhanced depression during sustained high frequency transmission. Consistently, both the first and second components of recovery are slower. (Hallermann et al., 2010) T bars are missing at the neuromuscular junction in mutant third instar larvae. (Fouquet et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	brp^5.45 (Fouquet et al., 2009, Hallermann et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Hallermann et al., 2010, J. Neurosci. 30(43): 14340--14345 Naked dense bodies provoke depression. [FBrf0214096] Fouquet et al., 2009, J. Cell Biol. 186(1): 129--145 Maturation of active zone assembly by Drosophila Bruchpilot. [FBrf0208461]

Allele Dmel\brp5.45

Allele Dmel\brp^5.45