Allele Dmel\Ced-12^19F3

General Information
Symbol	Dmel\Ced-12^19F3	Species	D. melanogaster
Name		FlyBase ID	FBal0266883
Feature type	allele	Associated gene	Dmel\Ced-12
Also Known As	elmo^19F3

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Geisbrecht et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Y393@. (Geisbrecht et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Ced-12^c06760) (Geisbrecht et al., 2008) lethal \| embryonic stage \| germline clone (Geisbrecht et al., 2008) neuroanatomy defective \| embryonic stage (Biersmith et al., 2011)
Phenotype Manifest In
	border follicle cell \| somatic clone (Geisbrecht et al., 2008) embryonic myoblast (with Df(2L)HO55) (Geisbrecht et al., 2008) presumptive embryonic/larval muscle system (with Df(2L)HO55) (Geisbrecht et al., 2008)
Detailed Description
	Statement Reference Embryos homozygous for Ced-12[19F3] exhibit minor defects in axonal patterning. Longitudinal fascicles show a nearly wild type pattern, while occasional thinning of these tracks and increased length of adjacent segments is observed. (Biersmith et al., 2011) Ced-12[19F3] border cell clones display sever migration defects, with 35% showing no migration and the remaining 65% arresting their journey when only 25% complete. Df(2L)HO55/Ced-12[19F3] embryos have a large number of unfused myoblasts as well as missing muscles. Germline clone embryos mutant for Ced-12[19F3] die prior to myogenesis. (Geisbrecht et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Ced-12^19F3 has neuroanatomy defective \| embryonic stage phenotype, enhanceable by Df(2L)CadN^Δ14 (Biersmith et al., 2011) Ced-12^19F3 has neuroanatomy defective \| embryonic stage phenotype, enhanceable by spg² (Biersmith et al., 2011)
Enhancer of
Statement Reference Ced-12^19F3 is an enhancer of neuroanatomy defective \| embryonic stage phenotype of spg² (Biersmith et al., 2011)
Phenotype Manifest In
Enhancer of
Statement Reference Ced-12^19F3 is an enhancer of lateral longitudinal fascicle phenotype of spg² (Biersmith et al., 2011)
Suppressor of
Statement Reference Ced-12[+]/Ced-12^19F3 is a suppressor of eye phenotype of Ced-12^Scer\UAS.cGa, Scer\GAL4^Mef2.PR, mbc^Scer\UAS.cBa (Geisbrecht et al., 2008)
Other
Statement Reference Ced-12^19F3, Df(2L)CadN^Δ14 has lateral longitudinal fascicle phenotype (Biersmith et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Compared to Ced-12[19F3]/Ced-12[19F3] or spg[2]/spg[2] single mutants, a consistent increase in longitudinal axon defects is observed in the double homozygous mutants. There is also an increase in axons that inappropriately cross the midline, and abnormalities in the spacing between adjacent segments is enhanced. The final muscle pattern in Ced-12[19F3]/Ced-12[19F3], spg[2]/spg[2] double mutant embryos appears wild type. Ced-12[19F3], Df(2L)CadN[Δ14] double mutant exhibit a consistent enhancement of axonal breaks, although no increase in midline guidance errors. These embryos also show collapsed outer longitudinal axon tracts onto the MP1 fascicle, a phenotype that is not observed in either single mutant. (Biersmith et al., 2011) The rough eye phenotype resulting from Scer\GAL4[GMR.PU]-mediated expression of Ced-12[Scer\UAS.cGa] and mbc[Scer\UAS.cBa] is suppressed by heterozygosity for Ced-12[19F3]. (Geisbrecht et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Ced-12^19F3 elmo^19F3 (Geisbrecht et al., 2008, Biersmith et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Biersmith et al., 2011, PLoS ONE 6(1): e16120 The DOCK Protein Sponge Binds to ELMO and Functions in Drosophila Embryonic CNS Development. [FBrf0212890] Geisbrecht et al., 2008, Dev. Biol. 314(1): 137--149 Drosophila ELMO/CED-12 interacts with Myoblast city to direct myoblast fusion and ommatidial organization. [FBrf0202723]

Allele Dmel\Ced-1219F3

Allele Dmel\Ced-12^19F3