FlyBase Allele Report: Dmel\Ced-12[19F3]

General Information

Symbol

Dmel\Ced-12^19F3

Species

D. melanogaster

Name

FlyBase ID

FBal0266883

Feature type

allele

Associated gene

Dmel\Ced-12

Associated Insertion(s)

Carried in Construct

Also Known As

elmo^19F3

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Geisbrecht et al., 2008)

Progenitor genotype

Cytology

Description

Amino acid replacement: Y393term.

(Geisbrecht et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:12,099,482..12,099,482 [+]

Nucleotide change:

T12099482A

Amino acid change:

Y393term | Ced-12-PA

Reported amino acid change:

Y393term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Could alternatively be a T to G nucleotide change; unspecified.

(Geisbrecht et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Biersmith et al., 2011)

lethal (with Ced-12^c06760)

(Geisbrecht et al., 2008)

lethal - all die during embryonic stage | germline clone

(Geisbrecht et al., 2008)

some die during embryonic stage | germline clone

(Geisbrecht et al., 2008)

Phenotype Manifest In

border follicle cell | somatic clone

(Geisbrecht et al., 2008)

embryonic myoblast (with Df(2L)HO55)

(Geisbrecht et al., 2008)

muscle attachment site | embryonic stage

(Liu et al., 2013)

presumptive embryonic/larval muscle system (with Df(2L)HO55)

(Geisbrecht et al., 2008)

tendon cell | embryonic stage

(Liu et al., 2013)

visceral muscle fiber | embryonic stage (with Ced-12^KO)

(Liu et al., 2013)

Detailed Description

Statement

Reference

Detached muscle fibres are present in Ced-12^19F3/Ced-12^KO zygotic mutant stage 16-17 embryos.

Stage 16-17 Ced-12^19F3/Ced-12^KO zygotic mutant embryos exhibit muscle attachment sites that are reduced in size and a decreased number of mature tendon cells.

(Liu et al., 2013)

Embryos homozygous for Ced-12^19F3 exhibit minor defects in axonal patterning. Longitudinal fascicles show a nearly wild type pattern, while occasional thinning of these tracks and increased length of adjacent segments is observed.

(Biersmith et al., 2011)

Ced-12^19F3 border cell clones display sever migration defects, with 35% showing no migration and the remaining 65% arresting their journey when only 25% complete.

Df(2L)HO55/Ced-12^19F3 embryos have a large number of unfused myoblasts as well as missing muscles.

Germline clone embryos mutant for Ced-12^19F3 die prior to myogenesis.

(Geisbrecht et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ced-12^19F3 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by spg²

(Biersmith et al., 2011)

Ced-12^19F3 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Df(2L)CadN^Δ14

(Biersmith et al., 2011)

Enhancer of

Statement

Reference

Ced-12^19F3 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of spg²

(Biersmith et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ced-12^KO/Ced-12^19F3 has visceral muscle fiber | embryonic stage phenotype, enhanceable by msk⁴/msk[+]

(Liu et al., 2013)

Enhancer of

Statement

Reference

Ced-12^19F3 is an enhancer of lateral longitudinal fascicle phenotype of spg²

(Biersmith et al., 2011)

Suppressor of

Statement

Reference

Ced-12[+]/Ced-12^19F3 is a suppressor of eye phenotype of Ced-12^UAS.cGa, Scer\GAL4^Mef2.PR, mbc^UAS.cBa

(Geisbrecht et al., 2008)

Other

Statement

Reference

Ced-12^19F3, Df(2L)CadN^Δ14 has lateral longitudinal fascicle phenotype

(Biersmith et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of msk⁴/+ increases the severity of muscle attachment defects in Ced-12^19F3/Ced-12^KO embryos.

(Liu et al., 2013)

Compared to Ced-12^19F3/Ced-12^19F3 or spg²/spg² single mutants, a consistent increase in longitudinal axon defects is observed in the double homozygous mutants. There is also an increase in axons that inappropriately cross the midline, and abnormalities in the spacing between adjacent segments is enhanced.

The final muscle pattern in Ced-12^19F3/Ced-12^19F3, spg²/spg² double mutant embryos appears wild type.

Ced-12^19F3, Df(2L)CadN^Δ14 double mutant exhibit a consistent enhancement of axonal breaks, although no increase in midline guidance errors. These embryos also show collapsed outer longitudinal axon tracts onto the MP1 fascicle, a phenotype that is not observed in either single mutant.

(Biersmith et al., 2011)

The rough eye phenotype resulting from Scer\GAL4^GMR.PU-mediated expression of Ced-12^Scer\UAS.cGa and mbc^Scer\UAS.cBa is suppressed by heterozygosity for Ced-12^19F3.

(Geisbrecht et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^Mef2.PR/Ced-12^UAS.cGa

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^how-24B/Ced-12^UAS.cGa

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^Mef2.PR/Ced-12^ΔN.UAS

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is partially rescued by Scer\GAL4^how-24B/Ced-12^ΔN.UAS

(Liu et al., 2013)

Not rescued by

Ced-12^KO/Ced-12^19F3 is not rescued by Scer\GAL4^sr-md710/Ced-12^UAS.cGa

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is not rescued by Ced-12^ΔC.UAS/Scer\GAL4^Mef2.PR

(Liu et al., 2013)

Ced-12^KO/Ced-12^19F3 is not rescued by Scer\GAL4^how-24B/Ced-12^ΔC.UAS

(Liu et al., 2013)

Comments

Scer\GAL4^sr-md710-mediated expression of Ced-12^Scer\UAS.cGa in tendon cells does not improve the muscle detachment phenotype of Ced-12^19F3/Ced-12^KO embryos.

Scer\GAL4^Mef2.PR- or Scer\GAL4^how-24B-mediated expression of Ced-12^Scer\UAS.cGa in muscles or mesoderm, respectively, efficiently but not completely rescues the muscle detachment phenotype of Ced-12^19F3/Ced-12^KO embryos.

Scer\GAL4^Mef2.PR- or Scer\GAL4^how-24B-mediated expression of Ced-12^ΔC.Scer\UAS in muscles or mesoderm, respectively, does not improve the muscle detachment phenotype of Ced-12^19F3/Ced-12^KO embryos.

(Liu et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Ced-12^19F3

ced-12^19F3

(Van Goethem et al., 2012)

ced-12^19f3

(Toret et al., 2018)

dced-12[19F3]

(Van Goethem et al., 2012)

elmo^19F3

(Liu et al., 2013, Biersmith et al., 2011, Geisbrecht et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (5)

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