UAS regulatory sequences drive expression of exd which is tagged at the N-terminal end with N-Venus. A linker of 5 amino acid residues separates the exd and N-Venus sequences. The fusion protein is also tagged with a Tag:HA epitope.
Scer\GAL4Ubx-M1/exdUAS.VNE.N-Venus,Tag:HA rescues exd1
Expression of exdScer\UAS.VNE.T:Avic\GFP-VN,T:Ivir\HA1 under the control of Scer\GAL4Ubx-M1 rescues the abdominal segment phenotypes of exd1 embryos.