Allele Dmel\DNApol-ε^NIG.6768R

General Information
Symbol	Dmel\DNApol-ε^NIG.6768R	Species	D. melanogaster
Name		FlyBase ID	FBal0267115
Feature type	allele	Associated gene	Dmel\DNApol-ε

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Verma et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..6 :9..502
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Carried in construct	P{NIG.6768R} (Verma et al., 2012, Suyari et al., 2012)
Associated Sequence Features	dsRNA-6768R
Cytology
Phenotypic Data
Phenotypic Class
	DNA repair defective \| third instar larval stage (Verma et al., 2012) endomitotic cell cycle defective, with Scer\GAL4^l(1)BP-1 (Suyari et al., 2012) lethal \| pupal stage, with Scer\GAL4^Act5C.PU (Suyari et al., 2012) lethal \| pupal stage, with Scer\GAL4^Act.PU (Suyari et al., 2012) mitotic cell cycle defective, with Scer\GAL4^ey.PU (Suyari et al., 2012) partially lethal - majority die \| pupal stage (Verma et al., 2012) partially lethal - majority die \| third instar larval stage (Verma et al., 2012) visible, with Scer\GAL4^ap-md544 (Suyari et al., 2012) visible, with Scer\GAL4^ey.PU (Suyari et al., 2012)
Phenotype Manifest In
	embryonic/larval brain \| third instar larval stage, with Scer\GAL4^Act5C.PU (Suyari et al., 2012) embryonic/larval salivary gland \| third instar larval stage, with Scer\GAL4^Act5C.PU (Suyari et al., 2012) eye, with Scer\GAL4^ey.PU (Suyari et al., 2012) eye disc, with Scer\GAL4^ey.PU (Suyari et al., 2012) imaginal disc \| third instar larval stage, with Scer\GAL4^Act5C.PU (Suyari et al., 2012) ommatidium, with Scer\GAL4^ey.PU (Suyari et al., 2012) salivary gland, with Scer\GAL4^l(1)BP-1 (Suyari et al., 2012) wing, with Scer\GAL4^ap-md544 (Suyari et al., 2012)
Detailed Description
	Statement Reference Expression of DNApol-ε[NIG.6768R] under the control of either Scer\GAL4[GMR.PS], Scer\GAL4[en-e16E], Scer\GAL4[sd.PU] or Scer\GAL4[Bx-MS1096] does not result in a detectable phenotype. Expression of DNApol-ε[NIG.6768R] under the control of Scer\GAL4[Act5C.PU] results in a reduction in size of the salivary gland, brain lobes and imaginal discs compared to wild type. Expression of DNApol-ε[NIG.6768R] under the control of Scer\GAL4[ey.PU] results in a small eye, with a reduction in the number of ommatidia. Incorporation of BrdU in the S phase zone posterior to the morphogenetic furrow is significantly reduced in eye imaginal discs in the animals. Expression of DNApol-ε[NIG.6768R] under the control of Scer\GAL4[ap-md544] results in an outstretched wing phenotype. Expression of DNApol-ε[NIG.6768R] under the control of Scer\GAL4[l(1)BP-1] results in a small salivary gland. The number and size of the salivary gland cell nuclei are decreased relative to wild type. The incorporation of BrdU in larvae 48 hours after hatching is severely decreased compared to wild type. (Suyari et al., 2012) Expression of DNApol-ε[NIG.6768R] under the control of Scer\GAL4[Act5C.PI] results in approximately 50% larval and pupal lethality. Larvae expressing DNApol-ε[NIG.6768R] under the control of Scer\GAL4[Act5C.PI] show increased sensitivity to DNA damage in response to bleomycin compared to wild type. (Verma et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference DNApol-ε^NIG.6768R has partially lethal - majority die \| pupal stage phenotype, enhanceable by l(3)pl10[+]/DNApol-ε^pl10R (Verma et al., 2012) DNApol-ε^NIG.6768R has partially lethal - majority die \| third instar larval stage phenotype, enhanceable by l(3)pl10[+]/DNApol-ε^pl10R (Verma et al., 2012)
Other
Statement Reference DNApol-α50^NIG.cUa, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-α60^NIG.5553R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype (Suyari et al., 2012) DNApol-α60^NIG.5553R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-α73^NIG.5923R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-α180^NIG.6349R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-δ^NIG.5949R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Iswi^NIG.8625R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Iswi^NIG.8625R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Mcm10^NIG.9241R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Mcm10^NIG.9241R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Psf2^NIG.18013R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Psf2^NIG.18013R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, RfC4^NIG.cUa, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012) DNApol-η^NIG.7143R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype (Suyari et al., 2012)
Phenotype Manifest In
Other
Statement Reference DNApol-α50^NIG.cUa, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-α60^NIG.5553R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-α73^NIG.5923R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-α180^NIG.6349R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-δ^NIG.5949R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Iswi^NIG.8625R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Mcm10^NIG.9241R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, Psf2^NIG.18013R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-ε^NIG.6768R, RfC4^NIG.cUa, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012) DNApol-η^NIG.7143R, DNApol-ε^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype (Suyari et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Flies co-expressing DNApol-ε[NIG.6768R] and any one of DNApol-α180[NIG.6349R], DNApol-α73[NIG.5923R], DNApol-α60[NIG.5553R], DNApol-α50[NIG.cUa], DNApol-δ[NIG.5949R], RfC4[NIG.cUa], Mcm10[NIG.9241R], Psf2[NIG.18013R], DNApol-η[NIG.7143R] or Iswi[NIG.8625R] under the control of Scer\GAL4[ey.PU] are headless. (Suyari et al., 2012) One copy of DNApol-ε[pl10R] enhances the lethality seen when DNApol-ε[NIG.6768R] is expressed under the control of Scer\GAL4[Act5C.PI], with only 23% of the expected progeny surviving to adulthood. (Verma et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	DNApol-ε^NIG.6768R is rescued by DNApol-ε^{Ct.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ey.PU (Suyari et al., 2012)
Comments	Co-expression of DNApol-ε[Ct.Scer\UAS.T:Ivir\HA1] rescues the small eye phenotype and mitotic cycle defects seen in the eye imaginal disc which are caused by expression of DNApol-ε[NIG.6768R] under the control of Scer\GAL4[ey.PU]. Co-expression of DNApol-ε[Ct.Scer\UAS.T:Ivir\HA1] does not rescue the endocycle defects seen in the salivary glands of animals expressing DNApol-ε[NIG.6768R] under the control of Scer\GAL4[l(1)BP-1]. (Suyari et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DNApol-ε^NIG.6768-R1 DNApol-ε^NIG.6768R
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Suyari et al., 2012, Gene 495(2): 104--114 Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle. [FBrf0217428] Verma et al., 2012, genesis 50(2): 86--101 DNApol‐ϵ gene is indispensable for the survival and growth of Drosophila melanogaster. [FBrf0217413]

Allele Dmel\DNApol-εNIG.6768R

Allele Dmel\DNApol-ε^NIG.6768R