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General Information
Symbol
Dmel\PGRP-LBΔ
Species
D. melanogaster
Name
FlyBase ID
FBal0267202
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:

PGRP-LB sequences were deleted by homologous recombination. A w+mW.hs marker which is flanked by loxP sites was inserted in place of the deleted sequence. The extentof the deletion was reported as 3R:7 ,278,564..7,286,342 in Release 5 coordinates.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Part of the PGRP-LB locus has been deleted. A w+mW.hs marker which is flanked by loxP sites is inserted in place of the deleted sequence.

Insertion components
TI{TI}PGRP-LBΔ
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

PGRP-LBΔ mutant adults have decreased lifespan and show reduced survival rate upon infection with Erwinia carotovora.

Mutant flies infected with Erwinia carotovora carotovora die after 2 weeks, in contrast to wild type.

After septic injury with the gram-negative E. carotovora bacterium, PGRP-LBΔ mutant flies exhibit a stronger and more sustained immune response than wild-type flies.

PGRP-LBΔ mutant flies show a strong response to oral E. carotovora, similar to that observed after infection by septic injury with the same bacteria, in contrast to wild-type flies.

PGRP-LBΔ flies infected with E. carotovora show reductions in mean lifespan compared to wild-type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The decreased lifespan at 29[o]C as well as the precocious lethality upon Erwinia carotovora infection characteristic for PGRP-LBΔ mutant adults is suppressed by combination with PGRP-SDsk1.

The reduced survival of PGRP-LBΔ flies after infection with Erwinia carotovora carotovora is suppressed if they are also mutant for PGRP-LE112.

After oral infection with E. carotovora, the addition of pirkEY00723 to PGRP-LBΔ; Df(3L)PGRP-SC1Δ results in an enhancement of the immune response locally and systemically. As with septic injury, pirkEY00723, PGRP-LBΔ; Df(3L)PGRP-SC1Δ flies beginning to die within 4 days of oral infection with E. carotovora.

pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ flies begin to die within 4 days of oral infection with E. carotovora. Although pirkEY00723 Df(2R)PGRP-SCΔ flies show the same level of response as pirkEY00723 flies, it is important to note that the response of pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ flies is significantly higher than that of pirkEY00723 Df(2R)PGRP-SCΔ flies.

The stronger immune responses to E. carotovora oral infection of Df(2R)PGRP-SCΔ PGRP-LBΔ flies is correlated with a further decrease in lifespan compared to single mutant flies. pirkEY00723 Df(2R)PGRP-SCΔ; PGRP-LBΔ mutant flies do not simply show an incremental reduction in their lifespan, but rather die rapidly with oral E. carotovora infection, decreasing 50% after only 5 days. These flies are also susceptible to oral infection with dead sonicated E. carotovora, demonstrating that is it not bacteria that are killing the fly but rather its own excessive immune response.

Dredd1; pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ and pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ, RelE20 flies, with impaired imd pathway activity due to the presence of Dredd or Rel mutations, exhibit an increased lifespan upon oral infection with E. carotovora bacteria, compared to pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ flies.

pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ and Df(2R)PGRP-SCΔ; PGRP-LBΔ flies in unchallenged conditions exhibit a marked reduction in lifespan.

The level of epithelium renewal, as evidenced by the number of mitotic cells along the midgut, is very high in pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ flies in the absence of bacterial infection, approaching the level seen in infected wild-type guts. The mitotic index of these flies doubles between unchallenged to E. carotovora oral infection conditions, suggesting that the level of epithelium renewal in the triple mutant is approaching the limit of cells available to undergo mitosis.

pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ and pirkEY00723, Df(2R)PGRP-SCΔ; PGRP-LBΔ, Df(3L)PGRP-SBΔ5 flies are viable, although not fully fertile.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (10)