Allele Dmel\PGRP-LB^Δ

General Information
Symbol	Dmel\PGRP-LB^Δ	Species	D. melanogaster
Name		FlyBase ID	FBal0267202
Feature type	allele	Associated gene	Dmel\PGRP-LB

Allele class
Mutagen	gene targeting by homologous recombination
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	gene targeting by homologous recombination (Paredes et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference PGRP-LB[Δ] was generated by homologous recombination. (Paredes et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Paredes et al., 2011) immune response defective (Paredes et al., 2011, Bosco-Drayon et al., 2012) immune response defective (with Df(3L)PGRP-SC1^Δ) (Paredes et al., 2011) immune response defective (with Df(3R)Exel8153) (Paredes et al., 2011) short lived (Paredes et al., 2011) short lived (with Df(3L)PGRP-SC1^Δ) (Paredes et al., 2011) viable (Paredes et al., 2011)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies infected with Erwinia carotovora carotovora die after 2 weeks, in contrast to wild type. (Bosco-Drayon et al., 2012) After septic injury with the gram-negative E. carotovora bacterium, PGRP-LB[Δ] mutant flies exhibit a stronger and more sustained immune response than wild-type flies. PGRP-LB[Δ] mutant flies show a strong response to oral E. carotovora, similar to that observed after infection by septic injury with the same bacteria, in contrast to wild-type flies. PGRP-LB[Δ] flies infected with E. carotovora show reductions in mean lifespan compared to wild-type. (Paredes et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference PGRP-LB^Δ/Df(3L)PGRP-SC1^Δ has immune response defective phenotype, enhanceable by pirk^EY00723 (Paredes et al., 2011) PGRP-LB^Δ/Df(3L)PGRP-SC1^Δ has short lived phenotype, enhanceable by pirk^EY00723 (Paredes et al., 2011)
Suppressed by
Statement Reference PGRP-LB^Δ has immune response defective phenotype, suppressible by PGRP-LE¹¹² (Bosco-Drayon et al., 2012)
Other
Statement Reference Df(2R)PGRP-SC^Δ, PGRP-LB^Δ, pirk^EY00723 has mitotic cell cycle defective phenotype (Paredes et al., 2011) PGRP-LB^Δ/Df(2R)PGRP-SC^Δ, Dredd¹, pirk^EY00723 has long lived phenotype (Paredes et al., 2011) PGRP-LB^Δ/Df(2R)PGRP-SC^Δ, Rel^E20, pirk^EY00723 has long lived phenotype (Paredes et al., 2011)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The reduced survival of PGRP-LB[Δ] flies after infection with Erwinia carotovora carotovora is suppressed if they are also mutant for PGRP-LE[112]. (Bosco-Drayon et al., 2012) After oral infection with E. carotovora, the addition of pirk[EY00723] to PGRP-LB[Δ]; Df(3L)PGRP-SC1[Δ] results in an enhancement of the immune response locally and systemically. As with septic injury, pirk[EY00723], PGRP-LB[Δ]; Df(3L)PGRP-SC1[Δ] flies beginning to die within 4 days of oral infection with E. carotovora. pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] flies begin to die within 4 days of oral infection with E. carotovora. Although pirk[EY00723] Df(2R)PGRP-SC[Δ] flies show the same level of response as pirk[EY00723] flies, it is important to note that the response of pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] flies is significantly higher than that of pirk[EY00723] Df(2R)PGRP-SC[Δ] flies. The stronger immune responses to E. carotovora oral infection of Df(2R)PGRP-SC[Δ] PGRP-LB[Δ] flies is correlated with a further decrease in lifespan compared to single mutant flies. pirk[EY00723] Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] mutant flies do not simply show an incremental reduction in their lifespan, but rather die rapidly with oral E. carotovora infection, decreasing 50% after only 5 days. These flies are also susceptible to oral infection with dead sonicated E. carotovora, demonstrating that is it not bacteria that are killing the fly but rather its own excessive immune response. Dredd[1]; pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] and pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ], Rel[E20] flies, with impaired imd pathway activity due to the presence of Dredd or Rel mutations, exhibit an increased lifespan upon oral infection with E. carotovora bacteria, compared to pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] flies. pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] and Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] flies in unchallenged conditions exhibit a marked reduction in lifespan. The level of epithelium renewal, as evidenced by the number of mitotic cells along the midgut, is very high in pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] flies in the absence of bacterial infection, approaching the level seen in infected wild-type guts. The mitotic index of these flies doubles between unchallenged to E. carotovora oral infection conditions, suggesting that the level of epithelium renewal in the triple mutant is approaching the limit of cells available to undergo mitosis. pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ] and pirk[EY00723], Df(2R)PGRP-SC[Δ]; PGRP-LB[Δ], Df(3L)PGRP-SB[Δ5] flies are viable, although not fully fertile. (Paredes et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	PGRP-LB^Δ is rescued by PGRP-LB^Scer\UAS.cGa/Scer\GAL4^c564 (Paredes et al., 2011) PGRP-LB^Δ is rescued by PGRP-LB^Scer\UAS.cGa/Scer\GAL4^da.G32 (Paredes et al., 2011) PGRP-LB^Δ is rescued by PGRP-LB^Scer\UAS.cGa/Scer\GAL4^NP1 (Paredes et al., 2011)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	PGRP-LB^Δ (Neyen et al., 2012, Paredes et al., 2011, Bosco-Drayon et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Bosco-Drayon et al., 2012, Cell Host Microbe 12(2): 153--165 Peptidoglycan Sensing by the Receptor PGRP-LE in the Drosophila Gut Induces Immune Responses to Infectious Bacteria and Tolerance to Microbiota. [FBrf0219236] Neyen et al., 2012, J. Immunol. 189(4): 1886--1897 Tissue- and Ligand-Specific Sensing of Gram-Negative Infection in Drosophila by PGRP-LC Isoforms and PGRP-LE. [FBrf0219094] Paredes et al., 2011, Immunity 35(5): 770--779 Negative Regulation by Amidase PGRPs Shapes the Drosophila Antibacterial Response and Protects the Fly from Innocuous Infection. [FBrf0216727]

Allele Dmel\PGRP-LBΔ

Allele Dmel\PGRP-LB^Δ