A significant proportion of HcfHR1
homozygous adults show ectopic wing veins and ectopic scutellar bristles, as compared to controls.
Homozygotes survive until the pupal stage, where they show approximately 50% lethality. Of those animals surviving to adulthood, 30% of males and 100% of females are sterile. Homozygous pupae and adults are smaller than normal. The wings are reduced in size, at least partly because of a reduction in cell size.
Homozygous wing discs show normal cell proliferation, but have increased levels of apoptosis compared to wild type.
The ovaries of homozygous females are usually underdeveloped. Egg chamber degeneration at around stage 8 of oogenesis is seen. The females lay some eggs, which are often fragile and smaller than normal, and have abnormally shaped anterior termini and dorsal appendages.
Homozygous adults show loss of humeral bristles (60% penetrance), held out wings (30% penetrance) and an ectopic vein initiating from the posterior crossvein in the wing (60% penetrance). Homozygous males have a reduced number of sex comb teeth (80% penetrance for 9 or fewer teeth/sex comb).