FB2025_02 , released April 17, 2025
Allele: Dmel\PGRP-LFRNAi.1.UAS
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General Information
Symbol
Dmel\PGRP-LFRNAi.1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0267520
Feature type
allele
Associated gene
Dmel\PGRP-LF
Associated Insertion(s)
Carried in Construct
P{UAS-PGRP-LF.RNAi.1}
Key Links
Transgenic product class
RNAi_reagent
(Maillet et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Maillet et al., 2008)
Mutagen
in vitro construct
(Maillet et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-PGRP-LF.RNAi.1}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of a fragment targeting the PGRP-LF z domain.

(Maillet et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PGRP-LF
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PGRP-LFdsRNA.1.Scer\UAS under the control of Scer\GAL4hs.PB results in constitutive, infection-independent activation of the IMD pathway, generating Dpt levels corresponding to approximately 20% of the full response after septic injury by E. coli. No increase in immune response nor its duration are seen in response to bacterial challenge compared to wild type.

      Expression of PGRP-LFdsRNA.1.Scer\UAS in the third instar larval trachea under the control of Scer\GAL4btl.PS results in infection-independent activation of the IMD pathway in the local immune response (seen as an increase in Drosomycin production).

      Flies expressing PGRP-LFdsRNA.1.Scer\UAS under the control of Scer\GAL4ptc-559.1 show wing scalloping at the distal tip. Expression using the Scer\GAL4en.PU driver results in notches in the posterior compartment of the wing.

      (Maillet et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      PGRP-LFRNAi.1.UAS, Scer\GAL4en.PU has visible | adult stage phenotype, enhanceable by key1

      (Maillet et al., 2008)
      Suppressed by
      Statement
      Reference

      PGRP-LFRNAi.1.UAS, Scer\GAL4ptc-559.1 has visible | adult stage phenotype, suppressible by PGRP-LCΔE

      (Maillet et al., 2008)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      PGRP-LFRNAi.1.UAS, Scer\GAL4en.PU has wing phenotype, enhanceable by key1

      (Maillet et al., 2008)
      Suppressed by
      Statement
      Reference

      PGRP-LFRNAi.1.UAS, Scer\GAL4ptc-559.1 has wing phenotype, suppressible by PGRP-LCΔE

      (Maillet et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A PGRP-LCΔE background suppresses the wing notches seen when PGRP-LFdsRNA.1.Scer\UAS is expressed under the control of Scer\GAL4ptc-559.1.

      A key1 background suppresses the wing notches seen when PGRP-LFdsRNA.1.Scer\UAS is expressed under the control of Scer\GAL4en.PU.

      (Maillet et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      PGRP-LFRNAi.1.UAS is partially rescued by PGRP-LFUAS.GFP/Scer\GAL4hs.PB

      (Maillet et al., 2008)
      Comments

      Expression of PGRP-LFScer\UAS.T:Avic\GFP partially rescues the infection-independent immune response seen when PGRP-LFdsRNA.1.Scer\UAS is expressed under the control of Scer\GAL4hs.PB.

      (Maillet et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      PGRP-LFRNAi.1.UAS
      PGRP-LFdsRNA.1.Scer\UAS
      PGRP-LFdsRNA.1.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)