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General Information
Symbol
Dmel\flwFP41
Species
D. melanogaster
Name
FlyBase ID
FBal0267524
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G10406571A

Amino acid change:

D222N | flw-PA; D91N | flw-PB; D91N | flw-PC; D91N | flw-PD

Reported amino acid change:

D222N, D91N

Comment:

The mutation was reported at position 222 relative to transcript RA and position 91 relative to transcript R-B.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A single point mutation results in replacement of an aspartic acid for a asparagine at amino acid 222 in orb2 isoform A and at amino acid 91 in isoform B.

Amino acid replacement: D222N.

Amino acid replacement: D91N.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous female germline clones result in small actin ring canals.

When posterior follicle cells (PFCs) are homozygous mutant for flwFP41 the oocyte nucleus often fails to relocalise and remains at the posterior pole. Oocyte polarity is unaffected when PFCs are wild-type and the anterior or lateral follicle cells are mutant for flwFP41. Some of the cells found in flwFP41 mutant PFC clones have small nuclei compared to controls and occasionally multiple cell layers are present at the posterior. Unlike in wild-type, mitotic PH3 positive PFCs can still be seen after stage 6. This overproliferation phenotype is never observed in clones induced at the lateral side of the epithelium.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

One copy of zip1 partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flwFP41.

One copy of Rho1E3.10 partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flwFP41.

One copy of RhoGEF204291 partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flwFP41.

Expression of NScer\UAS.cSb under the control of Scer\GAL4Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flwFP41.

Expression of NICN.Scer\UAS under the control of Scer\GAL4Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flwFP41.

Expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flwFP41.

Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4Act5C.PU in flwFP41 mutant follicle cell clones results in overproliferation in anterior follicle cells but not in lateral follicle cells. The amount of cell overproliferation in posterior follicle cells is increased compared to flwFP41 clones alone.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4Act5C.PU in flwFP41 mutant follicle cell clones results in overproliferation in anterior and lateral follicle cells. The amount of cell overproliferation in posterior follicle cells is increased compared to flwFP41 clones alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of flwScer\UAS.cRa under the control of Scer\GAL4tub.PU does not rescue the lethality of flwFP41.

Expression of flwScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4e22c rescues the oocyte polarity defects seen when the posterior follicle cells are mutant for flwFP41.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)