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General Information
Symbol
Dmel\garzΔ137
Species
D. melanogaster
Name
FlyBase ID
FBal0267832
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Approximate boundaries of 1825 bp deletion resulting from the imprecise excision of P{EPgy2}EY07592. The deletion extends from the site of the insertion into the garz gene.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{EPgy2}EY07592 results in a 1825bp deletion within the garz locus, removing the putative promoter region as well as the first exon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

garzΔ137 homozygous and garzΔ137/Df(2R)Exel6061 transheterozygous animals develop until the end of embryogenesis but fail to hatch and subsequently die. Moreover, mutant individuals exhibit asynchronous development and considerable retardation in comparison with wild-type controls.

garzΔ137 embryos exhibit a reduction in cis-Golgi network compartments in salivary gland cells. Vesicles secreting electron-dense ECM material, which are normally numerous close to the apical membrane of salivary gland cells at stage 16, are entirely absent in garzΔ137 mutant embryos. At the same time, the highly enriched rough endoplasmic reticulum (ER) is absent, with the ER appearing as a 'bloated balloon'. The formation of zonulae adherentes and septate junctions is not affected in garzΔ137 mutant embryos.

Salivary gland luminal length is unaffected in garzΔ137 mutant embryos.

Luminal deposition defects and protein retention in intracellular compartments, accompanied by a reduced luminal diameter are apparent in garzΔ137 mutant embryonic trachea. The clearance of liquid and replacement with gas are visible in wild-type animals as late as stage 16, but is inhibited in garzΔ137 mutants. The morphology of septate junctions appears wild-type in garzΔ137 mutants. Compared with wild-type, the apical membrane domains of tracheal cells in garzΔ137 mutant embryos are irregular and smaller. garzΔ137 mutant embryos exhibit severe morphological defects in cellular compartments, with malformed endoplasmic reticulae.

Before the death of homozygous garzΔ137 mutant individuals, the tracheal lumen collapses. Besides a reduction in luminal diameter, chitin cables organise aberrantly inside the tracheal lumen in garzΔ137 mutants.

Epidermal secretion of the cuticle is disturbed to various degrees in garzΔ137 mutants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

garzΔ137/+;Df(3L)Arl1-FRT/+ third instar larvae have significantly fewer boutons at the neuromuscular junction compared to garzΔ137/+ heterozygotes.

garzΔ137/+;Arfip71/+ or garzΔ137/+;Arfip12/+ third instar larvae have significantly fewer boutons at the neuromuscular junction compared to Arfip71/+ heterozygotes. garzΔ137/+ does not enhance the reduction in bouton number seen in Arfip71/Arfip12 larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)