Allele Dmel\rdhB¹

General Information
Symbol	Dmel\rdhB¹	Species	D. melanogaster
Name		FlyBase ID	FBal0267836
Feature type	allele	Associated gene	Dmel\rdhB

Allele class
Mutagen	ends-out gene targeting, FLPase, SCEI endonuclease
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-out gene targeting (Wang et al., 2012) FLPase (Wang et al., 2012) SCEI endonuclease (Wang et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The ends-out targeting construct deletes a ~500bp region encompassing the second to last exon and the translation termination site. (Wang et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Wang et al., 2012)
Phenotype Manifest In
	eye (Wang et al., 2012) rhabdomere (Wang et al., 2012)
Detailed Description
	Statement Reference rdhB[1] mutants are unable to produce rhodopsin after feeding on food containing either all-trans-retinal or all-trans-retinol. rdhB[1] mutants are able to synthesize normal levels of rhodopsin after being places on a diet supplemented with Β-carotene. However, in contrast to wild-type, levels of ninaE are reduced significantly in rdhB[1] mutants after feeding on either all-trans-retinal or all-trans-retinol. rdhB[1] mutants exhibit an electrophysiological phenotype due to low levels of rhodopsin resulting in a prolonged depolarization afterpotential (PDA). A PDA can only be produced when functional rhodopsin is in a molar excess over arrestin, as in 2 day old wild-type and rdhB[1] flies, and in 25 day old wild-type flies. Consistent with the 90% decrease in ninaE in 25 day old rdhB[1] mutants, bright blue light does not produce a PDA in the mutant. rdhB[1] mutants, aged under a light/dark cycle for 30 days, exhibit rhabdomeres that are either smaller than in wild-type or absent. All seven rhabodmeres are present if the mutant flies are incubated in the dark for 30 days. (Wang et al., 2012)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	rdhB¹ is rescued by Scer\GAL4^rdhB/rdhB^{Scer\UAS.T:Hsap\MYC} (Wang et al., 2012)
Comments	Expression of rdhB[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[rdhB] rescues the level of rhodopsin in the developing eye in rdhB[1] mutants to wild-type levels, so allowing bright blue light to produce a prolonged depolarization afterpotential, as in wild-type. (Wang et al., 2012)
Stocks ( 1 )
Bloomington	40977 cn¹ bw¹; rdhB¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	rdhB¹ (Wang et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Wang et al., 2012, J. Neurosci. 32(10): 3485--3491 The Drosophila Visual Cycle and De Novo Chromophore Synthesis Depends on rdhB. [FBrf0217693]

Allele Dmel\rdhB1

Allele Dmel\rdhB¹