Allele Dmel\Gk^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Gk^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0268041
Feature type	allele	Associated gene	Dmel\Gk

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Wightman et al., 2012) in vitro construct - RNAi (Wightman et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat of Gk sequences. (Wightman et al., 2012)
Carried in construct	P{UAS-Gk.IR} (Wightman et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	chemical sensitive, with Scer\GAL4^c564 (Wightman et al., 2012)
Phenotype Manifest In

Detailed Description
	Statement Reference Flies expressing Gk[dsRNA.Scer\UAS] under the control of Scer\GAL4[c564] have a lifespan similar to that of wild-type controls when raised on a sucrose only food source. However, when raised on a glycerol only food source, the mutant flies have a reduced lifespan compared to wild-type flies raised under these conditions, with the lifespan being similar to that under starvation conditions. The mutant flies show an intermediate lifespan when raised on a food source containing glycerol and sucrose. Adult males expressing Gk[dsRNA.Scer\UAS] under the control of Scer\GAL4[c564] have a small but significant decrease in survivorship on a high salt diet (3.5% and 4.0%) compared to control flies. (Wightman et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Gk^{dsRNA.Scer\UAS}, Scer\GAL4^c564 has chemical sensitive phenotype, suppressible by bw⁺/bw¹⁹ (Wightman et al., 2012)
NOT suppressed by
Statement Reference Gk^{dsRNA.Scer\UAS}, Scer\GAL4^c564 has chemical sensitive phenotype, non-suppressible by Gyk^Scer\UAS.Ex, Scer\GAL4^c564 (Wightman et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Gyk[Scer\UAS.Ex] does not rescue the reduced survival on a glycerol only food source of flies expressing Gk[dsRNA.Scer\UAS] under the control of Scer\GAL4[c564]. The glycerol hypersensitivity of flies expressing Gk[dsRNA.Scer\UAS] under the control of Scer\GAL4[c564] (seen as a reduction in survival on a glycerol only food source) is suppressed if the flies are heterozygous for bw[19]. (Wightman et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Gk^{dsRNA.Scer\UAS} is rescued by Gk^Scer\UAS.Ex/Scer\GAL4^c564 (Wightman et al., 2012)
Comments	Co-expression of Gk[Scer\UAS.Ex] rescues the reduced survival on a glycerol only food source of flies expressing Gk[dsRNA.Scer\UAS] under the control of Scer\GAL4[c564]. (Wightman et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Gk^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Wightman et al., 2012, PLoS ONE 7(3): e31779 Glycerol hypersensitivity in a Drosophila model for glycerol kinase deficiency is affected by mutations in eye pigmentation genes. [FBrf0217823]

Allele Dmel\GkdsRNA.Scer\UAS

Allele Dmel\Gk^{dsRNA.Scer\UAS}