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General Information
Symbol
Dmel\Gr66aUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lee
FlyBase ID
FBal0268064
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a Gr66a cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Gr8aScer\UAS.cLa, Gr98bScer\UAS.cSa and Gr66aScer\UAS.cLa in sugar-activated gustatory receptor neurons in L-type sensilla under the control of Scer\GAL4Gr64f.9.7 confers robust L-canavanine responses to these sensilla. Expression of only two of the three constructs is insufficient to confer significant L-canavadine responsiveness, although in some cases, co-expression of Gr8aScer\UAS.cLa and Gr66aScer\UAS.cLa produces a small L-canavanine-evoked response in L-type sensilla.

Expressing Gr8aScer\UAS.cLa, Gr98bScer\UAS.cSa and Gr66aScer\UAS.cLa in low salt-sensing gustatory receptor neurons under the control of Scer\GAL4Ir76b.1.5 confers significant L-canavanine responsiveness to these neurons. They do not induce responsiveness to any bitter compounds.

Expression of Gr8aScer\UAS.cLa, Gr98bScer\UAS.cSa and Gr66aScer\UAS.cLa under the control of Scer\GAL4Gr64f.9.7 in sweet-sensing gustatory receptor neurons enhances the loss of preference for sucrose over sucrose laced with L-canavanine seen in Df(3L)ex83 mutant flies: the flies now exhibit a significant attraction to the L-canavanine-containing food. No enhancement is seen when any two of the three constructs is expressed.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Gr66aScer\UAS.cLa under the control of either Scer\GAL4Gr66a.PD or Scer\GAL4Gr8a.0.7 rescues a normal avoidance response to L-canavanine in Df(3L)ex83 flies.

Expression of Gr66aScer\UAS.cLa under the control of either Scer\GAL4Gr66a.PD or Scer\GAL4Gr8a.0.7 restores normal L-canavanine-induced action potentials in the S3, S5 and S10 taste sensilla of Df(3L)ex83 flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Gr66aScer\UAS.cLa
Gr66aUAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Lee
Secondary FlyBase IDs
    References (2)