Allele Dmel\Apc2^{N-SAMP.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\Apc2^{N-SAMP.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0268077
Feature type	allele	Associated gene	Dmel\Apc2

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Zhou et al., 2011) in vitro construct - deletion (Zhou et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference T:Avic\GFP[EGFP] tagged truncated Apc2 (amino acids 1-490 plus 931-1067) is expressed under the control of the native promoter. (Zhou et al., 2011)
Carried in construct	P{Apc2-EGFP.N-SAMP} (Zhou et al., 2011)
Tagged with	T:Avic\GFP^EGFP (Zhou et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of Apc2[N-SAMP.T:Avic\GFP-EGFP] is unable to suppress the lethality seen in embryos derived from females carrying homozygous Apc2[g10] Apc[Q8] germline clones and heterozygous double mutant males (generated using the FRT-FLP-DFS technique). 17% of embryos are able to hatch and the severity of the cuticle defects is similar to in the double mutant alone. (Zhou et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Apc2^{N-SAMP.T:Avic\GFP-EGFP} partially rescues Apc2^g10 (Zhou et al., 2011)
Comments	Expression of Apc2[N-SAMP.T:Avic\GFP-EGFP] partially rescues the lethality seen in embryos that are both maternally and zygotically mutant for Apc2[g10], resulting in a 68% hatch rate. Fewer embryonic cuticle defects are also seen. Expression of Apc2[N-SAMP.T:Avic\GFP-EGFP] partially rescues the pseudocleavage furrow extension defects seen in embryos that are both maternally and zygotically mutant for Apc2[g10] (generated using the FRT-FLP-DFS technique). 41% of cortical actin rings in the four cortical divisions before cellularisation are incomplete. (Zhou et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Apc2^{N-SAMP.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Zhou et al., 2011, J. Cell Sci. 124(9): 1589--1600 Cortical localization of APC2 plays a role in actin organization but not in Wnt signaling in Drosophila. [FBrf0213457]

Allele Dmel\Apc2N-SAMP.T:Avic\GFP-EGFP

Allele Dmel\Apc2^{N-SAMP.T:Avic\GFP-EGFP}