Allele Dmel\Apc2^{R1-R5ExR.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\Apc2^{R1-R5ExR.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0268248
Feature type	allele	Associated gene	Dmel\Apc2

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Kunttas-Tatli et al., 2012) in vitro construct - coding region fusion (Kunttas-Tatli et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Full length Apc2 containing several amino acid replacements in the 20R1 and 20R5 regions (E595Q, Y600A, E603Q, T605A, P606A, T645A, D650A, L653A, T655A, P656A, D737N, F742A, E745Q, T747A, P748A, D784N, Y789A, E792Q, T794A, T795A, D862N, Y867A, E870Q, S872A, T873A) is expressed under the control of the native promoter and tagged with T:Avic\GFP[EGFP]. (Kunttas-Tatli et al., 2012)
Carried in construct	P{Apc2-EGFP.R1-R5ExR} (Kunttas-Tatli et al., 2012)
Tagged with	T:Avic\GFP^EGFP (Kunttas-Tatli et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference Apc2^{R1-R5ExR.T:Avic\GFP-EGFP} is a non-suppressor of lethal \| embryonic stage \| germline clone \| rescuable maternal effect phenotype of Apc2^g10, Apc^Q8 (Kunttas-Tatli et al., 2012)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Apc2^{R1-R5ExR.T:Avic\GFP-EGFP} is a non-suppressor of embryonic/larval cuticle \| germline clone \| rescuable maternal effect \| embryonic stage phenotype of Apc2^g10, Apc^Q8 (Kunttas-Tatli et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Expression of Apc2[R1-R5ExR.T:Avic\GFP-EGFP] fails to suppress the lethality seen in embryos derived from females carrying homozygous Apc2[g10] Apc[Q8] germline clones and heterozygous double mutant males (generated using the FRT-FLP-DFS technique). 21% of embryos are able to hatch. The severity of the cuticle defects in the remaining progeny is similar to the double mutant alone. (Kunttas-Tatli et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Apc2^{R1-R5ExR.T:Avic\GFP-EGFP} partially rescues Apc2^g10 (Kunttas-Tatli et al., 2012)
Comments	Expression of Apc2[R1-R5ExR.T:Avic\GFP-EGFP] modestly rescues the lethality seen in embryos that are both maternally and zygotically mutant for Apc2[g10], resulting in a 34% hatch rate. Weak cuticle defects are seen in the embryos that fail to hatch. (Kunttas-Tatli et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Apc2^{R1-R5ExR.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Kunttas-Tatli et al., 2012, Genetics 190(3): 1059--1075 Destruction complex function in the wnt signaling pathway of Drosophila requires multiple interactions between adenomatous polyposis coli 2 and armadillo. [FBrf0217816]

Allele Dmel\Apc2R1-R5ExR.T:Avic\GFP-EGFP

Allele Dmel\Apc2^{R1-R5ExR.T:Avic\GFP-EGFP}