Allele Dmel\RpS6^{dsRNA.Sym.Scer\UAS}

General Information
Symbol	Dmel\RpS6^{dsRNA.Sym.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0268400
Feature type	allele	Associated gene	Dmel\RpS6

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Lin et al., 2011) in vitro construct - RNAi (Lin et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences positioned at either end of the coding region drive expression of a 654bp RpS6 fragment in both sense and antisense orientation, resulting in the generation of dsRNA. (Lin et al., 2011)
Carried in construct	P{UAS-RpS6.RNAi} (Lin et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective, with Scer\GAL4^amn-c651 (Lin et al., 2011) large body \| heat sensitive, with Scer\GAL4^amn-c651, Scer\GAL80^ts.αTub84B (Lin et al., 2011) visible, with Scer\GAL4^GMR.PU (Lin et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PU (Lin et al., 2011) prothoracic gland, with Scer\GAL4^amn-c651 (Lin et al., 2011)
Detailed Description
	Statement Reference Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[GMR.PU] results in a smaller eye than normal which has a glassy appearance and necrotic patches. Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[ey.PU] does not result in an obvious mutant eye phenotype. Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[amn-c651] results in a reduction in the size of the prothoracic gland in larvae compared with controls at the equivalent time point of 5 days after egg deposition (AED). In addition, these animals show an extreme developmental delay compared to wild type: at day 5 AED the larvae are smaller than control larvae, and they continue to grow and feed beyond day 10 to become giant larvae that fail to pupate. Addition of 20-hydroxyecdysone to the food results in a variable restoration of pupariation, but the final steps of metamorphosis, including the formation of adult structures, are not rescued. Animals in which expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[amn-c651] is delayed until the late second larval instar (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele) are able to undergo pupation and eclose as large adults. These animals show an increase in eye size and in wing size compared to controls. If the animals are raised in the presence of 20-hydroxyecdysone, the body size of the adults is restored to normal. (Lin et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference RpS6^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^ey.PU is an enhancer of visible phenotype of CycE^JP (Lin et al., 2011)
Suppressor of
Statement Reference RpS6^{dsRNA.Sym.Scer\UAS}, Scer\GAL80^ts.αTub84B, Scer\GAL4^amn-c651 is a suppressor \| heat sensitive \| partially of visible phenotype of CycE^JP (Lin et al., 2011) Scer\GAL4^Act.PU/RpS6^{dsRNA.Sym.Scer\UAS} is a suppressor of visible phenotype of CycE^JP (Lin et al., 2011)
Phenotype Manifest In
Enhancer of
Statement Reference RpS6^{dsRNA.Sym.Scer\UAS}/Scer\GAL4^ey.PU is an enhancer of eye phenotype of CycE^JP (Lin et al., 2011)
Suppressor of
Statement Reference RpS6^{dsRNA.Sym.Scer\UAS}, Scer\GAL80^ts.αTub84B, Scer\GAL4^amn-c651 is a suppressor \| heat sensitive \| partially of eye phenotype of CycE^JP (Lin et al., 2011) Scer\GAL4^Act.PU/RpS6^{dsRNA.Sym.Scer\UAS} is a suppressor of eye phenotype of CycE^JP (Lin et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[GMR.PU] in a CycE[JP] homozygous background results in an additive reduction of eye size. Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[ey.PU] enhances the CycE[JP] rough eye phenotype. Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[Act.PU] suppresses the CycE[JP] eye phenotype. Expression of RpS6[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[amn-c651] from the late second larval instar (expression restricted at earlier stages using the temperature sensitive Scer\GAL80[ts.αTub84B] allele) partially suppresses the CycE[JP] small eye phenotype. (Lin et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	RpS6^{dsRNA.Sym.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Lin et al., 2011, PLoS Genet. 7(12): e1002408 Drosophila Ribosomal Protein Mutants Control Tissue Growth Non-Autonomously via Effects on the Prothoracic Gland and Ecdysone. [FBrf0217066]

Allele Dmel\RpS6dsRNA.Sym.Scer\UAS

Allele Dmel\RpS6^{dsRNA.Sym.Scer\UAS}