FlyBase Allele Report: Scer\GAL4[Act88F.PB]

General Information

Symbol

Scer\GAL4^Act88F.PB

Species

S. cerevisiae

Name

Actin 88F promoter construct of Bryantsev

FlyBase ID

FBal0268407

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Act88F-GAL4.B}

Also Known As

Act88F-GAL4, Actin88F-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Bryantsev et al., 2012)

Progenitor genotype

Carried in construct

P{Act88F-GAL4.B}

Cytology

Description

Act88F regulatory sequences from -1046 to -761 relative to the Act88F transcription start site are fused upstream of sequence encoding a GAL4 driver.

(Bryantsev et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Act88F

Act88F_IFM

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- adult stage

indirect flight muscle cell

(Bryantsev et al., 2012)

dorsal longitudinal indirect flight muscle cell

(Bryantsev et al., 2012)

dorsal ventral indirect flight muscle cell

(Bryantsev et al., 2012)

adult stage

indirect flight muscle cell

(Viswanathan et al., 2015)

indirect flight muscle

(Viswanathan et al., 2020)

Additional Information

Statement

Reference

Drives expression in dorsal medial and dorsal ventral indirect flight muscles.

(FlyBase Curators, 2017)

ScerGAL4^Act88F.PB drives expression in the indirect flight muscles, including the dorsal medial (DLM) and dorsal ventral (DVM) IFMs. starting at about 24 hours APF.

(Bryantsev et al., 2012)

Marker for

indirect flight muscle cell

(Bryantsev et al., 2012)

Reflects expression of

Act88F

(Viswanathan et al., 2015, Bryantsev et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with bru1^GD8699

(Oas et al., 2014)

abnormal flight, with bru1^GD17093

(Oas et al., 2014)

abnormal flight, with HnRNP-K^GD1382

(Oas et al., 2014)

abnormal flight, with mub^KK108209

(Oas et al., 2014)

abnormal flight, with Rbfox1^KK109100

(Nikonova et al., 2022)

abnormal flight, with Spf45^GD9411

(Oas et al., 2014)

abnormal flight | heat sensitive, with Act57B^UAS.cVa

(Viswanathan et al., 2020)

abnormal flight | progressive, with LamC^A177P.UAS

(Chandran et al., 2019)

abnormal jumping, with CG1674^GD14628

(Czajkowski et al., 2021)

abnormal jumping, with Rbfox1^JF02600

(Nikonova et al., 2022)

abnormal jumping, with Rbfox1^KK109100

(Nikonova et al., 2022)

abnormal size | adult stage, with LamC^G489V.UAS

(Chandran et al., 2019)

abnormal size | adult stage, with LamC^R205W.UAS

(Chandran et al., 2019)

atrophy | pupal stage, with hpo^UAS.cUa

(Kaya-Çopur et al., 2021)

decreased rate of flight behavior, with AMPKα^HMC04979

(Livelo et al., 2023)

decreased rate of flight behavior, with Gnmt^GD10563

(Livelo et al., 2023)

decreased rate of flight behavior, with Gnmt^HMS02473

(Livelo et al., 2023)

decreased rate of flight behavior, with Gnmt^KK107821

(Livelo et al., 2023)

decreased rate of flight behavior, with Nmdmc^HMC05275

(Livelo et al., 2023)

decreased rate of flight behavior, with Sardh^GD11909

(Livelo et al., 2023)

decreased rate of flight behavior, with Sardh^HMC03457

(Livelo et al., 2023)

decreased rate of flight behavior, with Sardh^KK101407

(Livelo et al., 2023)

decreased rate of flight behavior, with Sk2^GL00034

(Livelo et al., 2023)

decreased rate of flight behavior, with Tdh^GD5538

(Livelo et al., 2023)

decreased rate of flight behavior, with Tdh^HMC05585

(Livelo et al., 2023)

decreased rate of flight behavior, with Tdh^KK111666

(Livelo et al., 2023)

decreased rate of flight behavior | progressive, with Adsl^GD1722

(Livelo et al., 2023)

decreased size | adult stage, with Mlc2^KK111251

(Vaziri et al., 2020)

flightless, with Act57B^{K326Q.K328Q.UAS}

(Viswanathan et al., 2015)

flightless, with Act57B^K328Q.UAS

(Viswanathan et al., 2015)

flightless, with Act57B^UAS.GFP

(Viswanathan et al., 2015)

flightless, with CG1674^GD14628

(Czajkowski et al., 2021)

flightless, with CG1674^KK111975

(Czajkowski et al., 2021)

flightless, with Mlc2^KK111251

(Vaziri et al., 2020)

flightless, with Ogdh1^HMS00554

(González Morales et al., 2023)

flightless, with Zasp52^KK101276

(Chechenova et al., 2013)

flightless | heat sensitive, with Act57B^M306L.UAS

(Viswanathan et al., 2020)

flightless | progressive, with LamC^G489V.UAS

(Chandran et al., 2019)

flightless | progressive, with LamC^R205W.UAS

(Chandran et al., 2019)

flightless | progressive, with LamC^V528P.UAS

(Chandran et al., 2019)

increased rate of flight behavior, with Dgat2^KK109140

(Livelo et al., 2023)

partially lethal - majority live, with Rbfox1^KK109100

(Nikonova et al., 2022)

some die during P-stage, with Rbfox1^KK109100

(Nikonova et al., 2022)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Rbfox1^KK109100

(Nikonova et al., 2022)

dorsal longitudinal indirect flight muscle cell | heat sensitive, with Act57B^M306L.UAS

(Viswanathan et al., 2020)

dorsal longitudinal indirect flight muscle cell | pupal stage, with Mef2^GD5039

(Bryantsev et al., 2012)

dorsal longitudinal indirect flight muscle cell | pupal stage, with Mef2^RNAi.2.UAS

(Bryantsev et al., 2012)

flight muscle cell, with bru1^GD17093

(Oas et al., 2014)

H zone, with Zasp52^KK101276

(Katzemich et al., 2013)

indirect flight muscle, with Dgat2^KK109140

(Livelo et al., 2023)

indirect flight muscle, with Gnmt^GD10563

(Livelo et al., 2023)

indirect flight muscle, with Sardh^KK101407

(Livelo et al., 2023)

indirect flight muscle, with Tdh^HMC05585

(Livelo et al., 2023)

indirect flight muscle | adult stage, with Cs1^KK107737

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Dld^KK102614

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Dld^TKO.GS00548, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Dlst^HMC03051

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Dlst^TKO.GS03432, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Idh3a^GD5222

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Idh3a^KK107912

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Idh3b^GD6219

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Idh3g^GD6271

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Idh3g^KK102781

(González Morales et al., 2023)

indirect flight muscle | adult stage, with mAcon1^KK100999

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Mdh2^KK109040

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Ogdh1^GD4760

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Ogdh1^GD16611

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Ogdh1^HMS00554

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Ogdh1^TKO.GS00550, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

indirect flight muscle | adult stage, with Scsα1^KK102542

(González Morales et al., 2023)

indirect flight muscle cell, with Act57B^{K326Q.K328Q.UAS}

(Viswanathan et al., 2015)

indirect flight muscle cell, with Act88F^GD1353

(Bryantsev et al., 2012)

indirect flight muscle cell, with Actn^KK102286

(González-Morales et al., 2019)

indirect flight muscle cell, with hpo^UAS.cUa

(Kaya-Çopur et al., 2021)

indirect flight muscle cell, with Mlc2^KK111251

(Vaziri et al., 2020)

indirect flight muscle cell, with Zasp52^KK101276

(Chechenova et al., 2013, Katzemich et al., 2013)

indirect flight muscle cell, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

indirect flight muscle cell, with Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}

(González-Morales et al., 2019)

indirect flight muscle cell, with Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

indirect flight muscle cell, with Zasp67^{UAS.PE.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

indirect flight muscle cell | progressive, with Act57B^K328Q.UAS

(Viswanathan et al., 2015)

indirect flight muscle cell | pupal stage, with LamC^G489V.UAS

(Chandran et al., 2019)

indirect flight muscle cell | pupal stage, with LamC^R205W.UAS

(Chandran et al., 2019)

indirect flight muscle cell | pupal stage, with Mef2^GD5039

(Bryantsev et al., 2012)

indirect flight muscle cell | pupal stage, with Mef2^GD5039, Mef2^P544

(Bryantsev et al., 2012)

indirect flight muscle cell | pupal stage, with Mef2^GD5039, Mef2^RNAi.2.UAS

(Bryantsev et al., 2012)

lipid droplet | adult stage, with Dgat2^KK109140

(Livelo et al., 2023)

lipid droplet | adult stage, with Gnmt^GD10563

(Livelo et al., 2023)

lipid droplet | adult stage, with Sardh^KK101407

(Livelo et al., 2023)

lipid droplet | adult stage, with Tdh^HMC05585

(Livelo et al., 2023)

myofibril, with bru1^GD17093

(Oas et al., 2014)

myofibril, with Mlc2^KK111251

(Vaziri et al., 2020)

myofibril, with Zasp52^KK101276

(Chechenova et al., 2013)

myofibril | absent | adult stage, with mAcon1^KK100999

(González Morales et al., 2023)

myofibril | adult stage, with Cs1^KK107737

(González Morales et al., 2023)

myofibril | adult stage, with Dld^KK102614

(González Morales et al., 2023)

myofibril | adult stage, with Dld^TKO.GS00548, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

myofibril | adult stage, with Dlst^HMC03051

(González Morales et al., 2023)

myofibril | adult stage, with Dlst^TKO.GS03432, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

myofibril | adult stage, with Idh3a^GD5222

(González Morales et al., 2023)

myofibril | adult stage, with Idh3a^KK107912

(González Morales et al., 2023)

myofibril | adult stage, with Idh3b^GD6219

(González Morales et al., 2023)

myofibril | adult stage, with Idh3g^GD6271

(González Morales et al., 2023)

myofibril | adult stage, with Idh3g^KK102781

(González Morales et al., 2023)

myofibril | adult stage, with Mdh2^KK109040

(González Morales et al., 2023)

myofibril | adult stage, with Ogdh1^GD4760

(González Morales et al., 2023)

myofibril | adult stage, with Ogdh1^GD16611

(González Morales et al., 2023)

myofibril | adult stage, with Ogdh1^HMS00554

(González Morales et al., 2023)

myofibril | adult stage, with Ogdh1^TKO.GS00550, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

myofibril | adult stage, with Scsα1^KK102542

(González Morales et al., 2023)

myotube, with hpo^UAS.cUa

(Kaya-Çopur et al., 2021)

ribosome | absent | adult stage, with Ogdh1^HMS00554

(González Morales et al., 2023)

sarcomere, with bru1^GD17093

(Oas et al., 2014)

sarcomere, with Mlc2^KK111251

(Vaziri et al., 2020)

sarcomere | adult stage, with Actn^KK102286

(González-Morales et al., 2019)

sarcomere | adult stage, with Dld^TKO.GS00548, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

sarcomere | adult stage, with Dlst^TKO.GS03432, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

sarcomere | adult stage, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

sarcomere | adult stage, with Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}

(González-Morales et al., 2019)

sarcomere | pupal stage | pupal stage, with LamC^G489V.UAS

(Chandran et al., 2019)

sarcomere | pupal stage | pupal stage, with LamC^R205W.UAS

(Chandran et al., 2019)

Z disc, with bru1^GD17093

(Oas et al., 2014)

Z disc, with Zasp52^KK101276

(Chechenova et al., 2013, Katzemich et al., 2013)

Z disc | adult stage, with Actn^KK102286

(González-Morales et al., 2019)

Z disc | adult stage, with Ogdh1^GD4760

(González Morales et al., 2023)

Z disc | adult stage, with Ogdh1^GD16611

(González Morales et al., 2023)

Z disc | adult stage, with Ogdh1^HMS00554

(González Morales et al., 2023)

Z disc | adult stage, with Ogdh1^TKO.GS00550, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

(González Morales et al., 2023)

Z disc | adult stage, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp67^{UAS.PE.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Act57B^UAS.cVa, Scer\GAL4^Act88F.PB has abnormal flight | heat sensitive phenotype, enhanceable by Mhc¹⁰

(Viswanathan et al., 2020)

NOT suppressed by

Statement

Reference

Act57B^M306L.UAS, Scer\GAL4^Act88F.PB has flightless | heat sensitive phenotype, non-suppressible by Mhc¹⁰

(Viswanathan et al., 2020)

Enhancer of

Statement

Reference

TpnC4^KK110436, Scer\GAL4^Act88F.PB, Scer\GAL4^Act79B.PB is an enhancer of abnormal jump response phenotype of Scer\GAL4^Act79B.PB, TpnC41C^JF02379

(Chechenova et al., 2017)

Scer\GAL4^Act88F.PB, TpnC41C^JF02379, Scer\GAL4^Act79B.PB is an enhancer of abnormal jump response phenotype of Scer\GAL4^Act79B.PB, TpnC4^KK110436

(Chechenova et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has H zone phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has indirect flight muscle cell phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has Z disc phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Suppressed by

Statement

Reference

Act57B^M306L.UAS, Scer\GAL4^Act88F.PB has dorsal longitudinal indirect flight muscle cell | heat sensitive phenotype, suppressible by Mhc¹⁰

(Viswanathan et al., 2020)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has indirect flight muscle cell phenotype, suppressible | partially by Actn^KK102286, Scer\GAL4^Act88F.PB

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has sarcomere | adult stage phenotype, suppressible | partially by Actn^KK102286, Scer\GAL4^Act88F.PB

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has Z disc | adult stage phenotype, suppressible | partially by Actn^KK102286, Scer\GAL4^Act88F.PB

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has indirect flight muscle cell phenotype, suppressible by Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has Z disc | adult stage phenotype, suppressible by Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Enhancer of

Statement

Reference

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of H zone phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of indirect flight muscle cell phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of Z disc phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA} is a suppressor of Z disc | adult stage phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Actn^KK102286, Scer\GAL4^Act88F.PB is a suppressor | partially of indirect flight muscle cell phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Actn^KK102286, Scer\GAL4^Act88F.PB is a suppressor | partially of sarcomere | adult stage phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Actn^KK102286, Scer\GAL4^Act88F.PB is a suppressor | partially of Z disc | adult stage phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA} is a suppressor of indirect flight muscle cell phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Act88F.PB/tono^{UAS.ORF.GW.Tag:HA} rescues tono¹

(Zhang et al., 2024)

Scer\GAL4^Act88F.PB/tono^{UAS.ORF.GW.Tag:HA} rescues tono²

(Zhang et al., 2024)

Scer\GAL4^Act88F.PB/Zasp52^{UAS.PP.Tag:FLAG} rescues Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Partially rescues

Scer\GAL4^Act88F.PB/Zasp52^{Stop143.UAS.Tag:FLAG} partially rescues Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99925

P{Act88F-GAL4.B}1, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Act88F.PB

Name Synonyms

Actin 88F promoter construct of Bryantsev

Secondary FlyBase IDs

References (30)

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