FlyBase Allele Report: Scer\GAL4[Act88F.PB]

General Information

Symbol

Scer\GAL4^Act88F.PB

Species

S. cerevisiae

Name

Actin 88F promoter construct of Bryantsev

FlyBase ID

FBal0268407

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Act88F-GAL4.B}

Also Known As

Act88F-GAL4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Bryantsev et al., 2012)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{Act88F-GAL4.B}

Cytology

Nature of the lesion

Statement

Reference

Act88F regulatory sequences from -1046 to -761 relative to the Act88F transcription start site are fused upstream of sequence encoding a GAL4 driver.

(Bryantsev et al., 2012)

Allele components

Product class / Tool use(s)

Regulatory region(s)

Act88F

Encoded product / tool

GAL4

binary expression system - driver

Associated Sequence Features

Act88F_IFM

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

indirect flight muscle

(Viswanathan et al., 2015)

pupal stage P6 -- adult stage

indirect flight muscle

(Bryantsev et al., 2012)

dorsal medial indirect flight muscle

(Bryantsev et al., 2012)

dorsal ventral indirect flight muscle

(Bryantsev et al., 2012)

Additional Information

Statement

Reference

Scer\GAL4^Act88F.PB drives expression in the indirect flight muscles, including the dorsal medial (DLM) and dorsal ventral (DVM) IFMs. starting at about 24 hours APF.

(Bryantsev et al., 2012)

Marker for

indirect flight muscle

(Bryantsev et al., 2012)

Reflects expression of

Act88F

(Viswanathan et al., 2015, Bryantsev et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

flight defective, with bru1^GD8699

(Oas et al., 2014)

flight defective, with bru1^GD17093

(Oas et al., 2014)

flight defective, with HnRNP-K^GD1382

(Oas et al., 2014)

flight defective, with mub^KK108209

(Oas et al., 2014)

flight defective, with Spf45^GD9411

(Oas et al., 2014)

flight defective | progressive, with LamC^A177P.UAS

(Chandran et al., 2019)

flightless, with Act57B^{K326Q.K328Q.UAS}

(Viswanathan et al., 2015)

flightless, with Act57B^K328Q.UAS

(Viswanathan et al., 2015)

flightless, with Act57B^UAS.GFP

(Viswanathan et al., 2015)

flightless, with Zasp52^KK101276

(Chechenova et al., 2013)

flightless | progressive, with LamC^G489V.UAS

(Chandran et al., 2019)

flightless | progressive, with LamC^R205W.UAS

(Chandran et al., 2019)

flightless | progressive, with LamC^V528P.UAS

(Chandran et al., 2019)

size defective | adult stage, with LamC^G489V.UAS

(Chandran et al., 2019)

size defective | adult stage, with LamC^R205W.UAS

(Chandran et al., 2019)

Phenotype Manifest In

dorsal medial indirect flight muscle | pupal stage, with Mef2^{dsRNA.IR2.UAS}

(Bryantsev et al., 2012)

dorsal medial indirect flight muscle | pupal stage, with Mef2^GD5039

(Bryantsev et al., 2012)

flight muscle, with bru1^GD17093

(Oas et al., 2014)

H zone, with Zasp52^KK101276

(Katzemich et al., 2013)

indirect flight muscle, with Act57B^{K326Q.K328Q.UAS}

(Viswanathan et al., 2015)

indirect flight muscle, with Act88F^GD1353

(Bryantsev et al., 2012)

indirect flight muscle, with Actn^KK102286

(González-Morales et al., 2019)

indirect flight muscle, with Zasp52^KK101276

(Chechenova et al., 2013, Katzemich et al., 2013)

indirect flight muscle, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

indirect flight muscle, with Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}

(González-Morales et al., 2019)

indirect flight muscle, with Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

indirect flight muscle, with Zasp67^{UAS.PE.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

indirect flight muscle | progressive, with Act57B^K328Q.UAS

(Viswanathan et al., 2015)

indirect flight muscle | pupal stage, with LamC^G489V.UAS

(Chandran et al., 2019)

indirect flight muscle | pupal stage, with LamC^R205W.UAS

(Chandran et al., 2019)

indirect flight muscle | pupal stage, with Mef2^GD5039

(Bryantsev et al., 2012)

indirect flight muscle | pupal stage, with Mef2^GD5039, Mef2^{dsRNA.IR2.UAS}

(Bryantsev et al., 2012)

indirect flight muscle | pupal stage, with Mef2^GD5039, Mef2^P544

(Bryantsev et al., 2012)

myofibril, with bru1^GD17093

(Oas et al., 2014)

myofibril, with Zasp52^KK101276

(Chechenova et al., 2013)

sarcomere, with bru1^GD17093

(Oas et al., 2014)

sarcomere | adult stage, with Actn^KK102286

(González-Morales et al., 2019)

sarcomere | adult stage, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

sarcomere | adult stage, with Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}

(González-Morales et al., 2019)

sarcomere | pupal stage | pupal stage, with LamC^G489V.UAS

(Chandran et al., 2019)

sarcomere | pupal stage | pupal stage, with LamC^R205W.UAS

(Chandran et al., 2019)

Z disc, with bru1^GD17093

(Oas et al., 2014)

Z disc, with Zasp52^KK101276

(Chechenova et al., 2013, Katzemich et al., 2013)

Z disc | adult stage, with Actn^KK102286

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp52^{ΔPDZ.UAS.PR.Tag:FLAG}

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Z disc | adult stage, with Zasp67^{UAS.PE.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

TpnC4^KK110436, Scer\GAL4^Act88F.PB, Scer\GAL4^Act79B.PB is an enhancer of jump response defective phenotype of Scer\GAL4^Act79B.PB, TpnC41C^JF02379

(Chechenova et al., 2017)

Scer\GAL4^Act88F.PB, TpnC41C^JF02379, Scer\GAL4^Act79B.PB is an enhancer of jump response defective phenotype of Scer\GAL4^Act79B.PB, TpnC4^KK110436

(Chechenova et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has H zone phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has indirect flight muscle phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has Z disc phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has indirect flight muscle phenotype, suppressible | partially by Actn^KK102286, Scer\GAL4^Act88F.PB

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has indirect flight muscle phenotype, suppressible by Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has Z disc | adult stage phenotype, suppressible by Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA}

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has sarcomere | adult stage phenotype, suppressible | partially by Actn^KK102286, Scer\GAL4^Act88F.PB

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP has Z disc | adult stage phenotype, suppressible | partially by Actn^KK102286, Scer\GAL4^Act88F.PB

(González-Morales et al., 2019)

Enhancer of

Statement

Reference

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of H zone phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of indirect flight muscle phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB/Zasp66^KK112973 is an enhancer of Z disc phenotype of Scer\GAL4^Act88F.PB, Zasp52^KK101276

(Katzemich et al., 2013)

Suppressor of

Statement

Reference

Actn^KK102286, Scer\GAL4^Act88F.PB is a suppressor | partially of indirect flight muscle phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA} is a suppressor of indirect flight muscle phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Scer\GAL4^Act88F.PB/Zasp66^{UAS.PK.Tag:FLAG.Tag:HA} is a suppressor of Z disc | adult stage phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Actn^KK102286, Scer\GAL4^Act88F.PB is a suppressor | partially of sarcomere | adult stage phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Actn^KK102286, Scer\GAL4^Act88F.PB is a suppressor | partially of Z disc | adult stage phenotype of Scer\GAL4^Act88F.PB, Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Act88F.PB/Zasp52^{UAS.PP.Tag:FLAG} rescues Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Partially rescues

Scer\GAL4^Act88F.PB/Zasp52^{Stop143.UAS.Tag:FLAG} partially rescues Zasp52^UAS.PR.GFP

(González-Morales et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Act88F.PB

Name Synonyms

Actin 88F promoter construct of Bryantsev

Secondary FlyBase IDs

References (14)

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