FlyBase Allele Report: Dmel\upd1[UAS.cUa]

General Information

Symbol

Dmel\upd1^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0268408

Feature type

allele

Associated gene

Dmel\upd1

Associated Insertion(s)

Carried in Construct

P{UAS-upd1.U}

Also Known As

UAS-upd, UAS-upd1

Key Links

Transgenic product class

wild_type

(Classen et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Classen et al., 2009)

Mutagen

in vitro construct

(Classen et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-upd1.U}

Cytology

Description

UAS regulatory sequences drive expression of upd1.

(Classen et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

upd1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Wu et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^P0206

(Romão et al., 2021)

abnormal immune response | adult stage, with Scer\GAL4^r4

(Shen et al., 2022)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Luo and Sehgal, 2012)

abnormal locomotor rhythm | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Luo and Sehgal, 2012)

abnormal size | third instar larval stage, with Scer\GAL4^ptc.PU

(Recasens-Alvarez et al., 2017)

decreased cell number | adult stage | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2020)

hyperplasia, with Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

increased cell number | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2020)

increased mortality, with Scer\GAL4^r4

(Shen et al., 2022)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2020, Nie et al., 2015)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Perea et al., 2017, Jiang et al., 2011)

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP5130

(Nie et al., 2015)

adult midgut epithelium | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2020)

adult thoracic mechanosensory chaeta, with Scer\GAL4^dpp.blk1

(Worley et al., 2018)

adult thoracic mechanosensory chaeta, with Scer\GAL4^GMR15B03

(Worley et al., 2018)

adult thorax, with Scer\GAL4^dpp.blk1

(Worley et al., 2018)

adult thorax, with Scer\GAL4^GMR15B03

(Worley et al., 2018)

enteroblast | adult stage | decreased number, with Scer\GAL4^NP5130

(Jin et al., 2020)

intestinal stem cell | adult stage, with Scer\GAL4^NP7397

(Jiang et al., 2011)

intestinal stem cell | adult stage | increased number, with Scer\GAL4^NP5130

(Jin et al., 2020)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP7397

(Perea et al., 2017)

mesothoracic tergum primordium | third instar larval stage, with Scer\GAL4^ptc.PU

(Recasens-Alvarez et al., 2017)

wing disc, with Scer\GAL4^Bx-MS1096

(Classen et al., 2009)

wing disc, with Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

wing pouch | third instar larval stage, with Scer\GAL4^ptc.PU

(Recasens-Alvarez et al., 2017)

Detailed Description

Statement

Reference

Adulthood-only expression of upd1^UAS.cUa under the control of Scer\GAL4^NP5130 (and GAL80[ts] for the temporal control of expression) induces an increase in the number of progenitor cells in the midgut, as well as an increase in mitotic index.

(Jin et al., 2020)

Expression of upd1^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) does not lead to ectopic wing pouches in wing discs or ectopic wings when compared to controls.

Expression of upd1^UAS.cUa under the control of Scer\GAL4^GMR15B03 or Scer\GAL4^dpp.blk1 leads to disruption of thorax and loss of many mechanosensory chaetas when compared to control adults.

(Worley et al., 2018)

Adult flies expressing upd1^Scer\UAS.cUa driven by Scer\GAL4^esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display significant increase in midgut stem cell proliferation (assessed by number of pH3-positive cells).

(Perea et al., 2017)

The expression of upd1^Scer\UAS.cUa under the control of Scer\GAL4^ptc.PU leads to a significant decrease in the size of mesonotum primordia size, from which ectopic wing primordium structures frequently emerge, as compared to controls.

(Recasens-Alvarez et al., 2017)

Expression of upd1^Scer\UAS.cUa under the control of Scer\GAL4^esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant increase in the number of mitotic cells in the midgut, as compared to controls.

(Nie et al., 2015)

Scer\GAL4^ptc-559.1-mediated expression of upd1^Scer\UAS.cUa results in overgrown wing discs.

(Djiane et al., 2013)

Expression of upd1^Scer\UAS.cUa under the control of Scer\GAL4^tim.PE results in arrhythmic locomotor activity under constant darkness conditions.

Expression of upd1^Scer\UAS.cUa under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 results in defects in locomotor activity rhythms (only 33.3% of flies show rhythmic behaviour under constant darkness conditions).

(Luo and Sehgal, 2012)

Expression of upd1^Scer\UAS.cUa under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B induces intestinal stem cell proliferation.

(Jiang et al., 2011)

No tumors are seen when upd1^Scer\UAS.cUa is expressed in eye-antennal clones under the control of Scer\GAL4^Act.PU. Inhibition of apoptosis in upd1^Scer\UAS.cUa-expressing cells does not induce tumors.

(Wu et al., 2010)

Wing discs expressing upd1^Scer\UAS.cUa under the control of Scer\GAL4^Bx-MS1096 show an expansion of the disc epithelium and increased epithelial folding.

(Classen et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has visible | adult stage phenotype, enhanceable by CtBP[+]/CtBP^87De-10

(Worley et al., 2018)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has neoplasia | somatic clone phenotype, enhanceable by upd2^UAS.cUa

(Wu et al., 2010)

NOT Enhanced by

Statement

Reference

CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has visible | adult stage phenotype, non-enhanceable by upd2^Δ/upd2[+]/upd3^Δ/upd3[+]

(Worley et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, upd1^UAS.cUa has increased cell number | adult stage phenotype, suppressible by Sox100B^NIG.15552R/Scer\GAL4^NP5130

(Jin et al., 2020)

Scer\GAL4^NP5130, upd1^UAS.cUa has increased occurrence of cell division | adult stage phenotype, suppressible by Sox100B^NIG.15552R/Scer\GAL4^NP5130

(Jin et al., 2020)

Scer\GAL4^NP0001, upd1^UAS.cUa has increased occurrence of cell division | adult stage phenotype, suppressible by Krn^s.UAS.cRa, Scer\GAL4^NP0001

(Jiang et al., 2011)

Scer\GAL4^NP7397, upd1^UAS.cUa has increased occurrence of cell division | adult stage phenotype, suppressible by Ras85D^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Jiang et al., 2011)

Scer\GAL4^NP7397, upd1^UAS.cUa has increased occurrence of cell division | adult stage phenotype, suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Jiang et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has neoplasia | somatic clone phenotype, suppressible by dome^ΔCYT.UAS, Scer\GAL4^Act.PU

(Wu et al., 2010)

NOT suppressed by

Statement

Reference

CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has visible | adult stage phenotype, non-suppressible by upd2^Δ/upd2[+]/upd3^Δ/upd3[+]

(Worley et al., 2018)

Scer\GAL4^NP7397, upd1^UAS.cUa has increased occurrence of cell division | adult stage phenotype, non-suppressible by Ret^GD45, Scer\GAL4^NP7397

(Perea et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has neoplasia | somatic clone phenotype, non-suppressible by bsk^DN.UAS, Scer\GAL4^Act.PU

(Wu et al., 2010)

Enhancer of

Statement

Reference

upd1^UAS.cUa, Scer\GAL4^rn-GAL4-5 is an enhancer of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd1^UAS.cUa, Scer\GAL4^rn-GAL4-5 is an enhancer of visible | adult stage phenotype of CtBP^87De-10, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^dpp.blk1/upd1^UAS.cUa is a suppressor of visible | adult stage phenotype of Scer\GAL4^dpp.blk1, bi^UAS.cPa

(Tsai et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hh.PU/upd1^UAS.cUa is a non-suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Other

Statement

Reference

Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has visible | adult stage phenotype

(Worley et al., 2018)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has neoplasia | somatic clone phenotype

(Wu et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has wing | ectopic phenotype, enhanceable by CtBP[+]/CtBP^87De-10

(Worley et al., 2018)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has eye disc | somatic clone phenotype, enhanceable by upd2^UAS.cUa

(Wu et al., 2010)

NOT Enhanced by

Statement

Reference

CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has wing | ectopic phenotype, non-enhanceable by upd2^Δ/upd2[+]/upd3^Δ/upd3[+]

(Worley et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, upd1^UAS.cUa has intestinal stem cell | increased number | adult stage phenotype, suppressible by Sox100B^NIG.15552R/Scer\GAL4^NP5130

(Jin et al., 2020)

Scer\GAL4^NP5130, upd1^UAS.cUa has adult midgut epithelium | adult stage phenotype, suppressible by Sox100B^NIG.15552R/Scer\GAL4^NP5130

(Jin et al., 2020)

Scer\GAL4^NP5130, upd1^UAS.cUa has enteroblast | adult stage | decreased number phenotype, suppressible by Sox100B^NIG.15552R/Scer\GAL4^NP5130

(Jin et al., 2020)

Scer\GAL4^NP0001, upd1^UAS.cUa has intestinal stem cell | adult stage phenotype, suppressible by Krn^s.UAS.cRa, Scer\GAL4^NP0001

(Jiang et al., 2011)

Scer\GAL4^NP7397, upd1^UAS.cUa has intestinal stem cell | adult stage phenotype, suppressible by Ras85D^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Jiang et al., 2011)

Scer\GAL4^NP7397, upd1^UAS.cUa has intestinal stem cell | adult stage phenotype, suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Jiang et al., 2011)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has eye disc | somatic clone phenotype, suppressible by dome^ΔCYT.UAS, Scer\GAL4^Act.PU

(Wu et al., 2010)

NOT suppressed by

Statement

Reference

CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has wing | ectopic phenotype, non-suppressible by upd2^Δ/upd2[+]/upd3^Δ/upd3[+]

(Worley et al., 2018)

Scer\GAL4^NP7397, upd1^UAS.cUa has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by Ret^GD45, Scer\GAL4^NP7397

(Perea et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has eye disc | somatic clone phenotype, non-suppressible by bsk^DN.UAS, Scer\GAL4^Act.PU

(Wu et al., 2010)

Enhancer of

Statement

Reference

upd1^UAS.cUa, Scer\GAL4^rn-GAL4-5 is an enhancer of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd1^UAS.cUa, Scer\GAL4^rn-GAL4-5 is an enhancer of wing | ectopic phenotype of CtBP^87De-10, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd1^UAS.cUa, Scer\GAL4^rn-GAL4-5 is an enhancer of wing pouch | third instar larval stage | ectopic phenotype of CtBP^87De-10, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^dpp.blk1/upd1^UAS.cUa is a suppressor of eye | absent phenotype of Scer\GAL4^dpp.blk1, bi^UAS.cPa

(Tsai et al., 2015)

Scer\GAL4^dpp.blk1/upd1^UAS.cUa is a suppressor of eye disc | third instar larval stage phenotype of Scer\GAL4^dpp.blk1, bi^UAS.cPa

(Tsai et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hh.PU/upd1^UAS.cUa is a non-suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Other

Statement

Reference

Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa has wing | ectopic phenotype

(Worley et al., 2018)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Katheder et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa has eye disc | somatic clone phenotype

(Wu et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of upd1^UAS.cUa and egr^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) leads to ectopic wings compared to controls.

Expression of upd1^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP^87De-10/+ background does not lead to ectopic wing pouches in wing discs or ectopic wings when compared to controls.

(Worley et al., 2018)

The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing upd1^Scer\UAS.cUa driven by Scer\GAL4^esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is not suppressed by co-expression of Ret^GD45.

(Perea et al., 2017)

Co-expression of bun^GD1392 suppresses the increased number of mitotic cells seen in the midguts of flies expressing upd1^Scer\UAS.cUa under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

Intestinal stem cell proliferation found in flies expressing upd1^Scer\UAS.cUa under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B is completely inhibited when Egfr or Ras85D is down-regulated (through expression of either Egfr^{dsRNA.Scer\UAS.cUa} or Ras85D^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B).

Co-expression of upd1^Scer\UAS.cUa and spi^s.Scer\UAS in intestinal stem cells, under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B induces a much higher intestinal stem cell mitotic index than that induced by the activation of either transgene alone.

(Jiang et al., 2011)

No tumors are seen when upd1^Scer\UAS.cUa is expressed under the control of Scer\GAL4^Act.PU in scrib¹ mutant eye-antennal clones.

No tumors are seen when upd1^Scer\UAS.cUa is expressed under the control of Scer\GAL4^Act.PU in wild type eye-antennal disc cells that are adjacent to scrib¹ mutant cells.

Eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS and upd1^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU grow into large invasive tumors.

Eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS, upd1^Scer\UAS.cUa and upd2^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU grow into large invasive tumors that are larger than those seen when Ras85D^V12.Scer\UAS is co-expressed with either upd1^Scer\UAS.cUa or upd2^Scer\UAS.cUa alone.

Expression of bsk^DN.Scer\UAS does not suppress the tumors seen in eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS and upd1^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU.

Expression of dome^{ΔCYT.Scer\UAS} in eye-antennal disc clones that express Ras85D^V12.Scer\UAS and upd1^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU suppresses the overgrowth and invasion of the nerve cord.

(Wu et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/upd1^UAS.cUa rescues upd1^JF03149

(Beshel et al., 2017)

Comments

The increased attraction to food cues, increased food consumption and higher body weight of adult flies expressing upd1^JF03149 under the control of Scer\GAL4^elav.PU can be rescued by expression of upd1^Scer\UAS.cUa.

(Beshel et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

os^Scer\UAS.cUa

upd1^Scer\UAS.cUa

upd1^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (34)

Report Sections

Open Close