FB2025_01 , released February 20, 2025
Allele: Dmel\Gαq961
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General Information
Symbol
Dmel\Gαq961
Species
D. melanogaster
Name
FlyBase ID
FBal0268413
Feature type
allele
Associated gene
Dmel\Gαq
Associated Insertion(s)
Carried in Construct
Also Known As
Gαq961
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Hu et al., 2012)
Mutagen
ethyl methanesulfonate
(Hu et al., 2012)
Progenitor genotype
Cytology
Description

Mutation is within in exon 4 of the Gα49B 1 isoform (this exon is not included in the 3 and 4 isoforms).

(Hu et al., 2012)

Nucleotide substitution: C961T.

(Hu et al., 2012)

Amino acid replacement: R177term.

(Hu et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:12,617,526..12,617,526 [+]
Nucleotide change:

C12617526T

Reported nucleotide change:

C961T

Amino acid change:

R177term | Galphaq-PD; R220term | Galphaq-PJ

Reported amino acid change:

R177term

(Hu et al., 2012)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous adults have small electroretinogram (ERG) responses and slow response termination compared to wild type. The on and off transients are reduced and the mutant flies show a significant reduction in light sensitivity. Intracellular recordings demonstrate that the defect in light response is due to an abnormality in the photoreceptor cells.

      Gα49B1/Gα49B961 and Gα49B961/Df(2R)Exel7121 adults show a defective ERG response.

      The minimum light intensity needed to induce a prolonged depolarization afterpotential is approximately 420 lux in homozygous flies (compared to approximately 2600 lux in wild type).

      Mutants undergo slow light-dependent retinal degeneration.

      (Hu et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The deactivation of the electroretinogram photoresponse is much faster than in the Gα49B961 mutant in Gα49B961 ninaE8 double mutant flies.

      (Hu et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Gαq1

      (Hu et al., 2012)
      Rescued by

      Gαq961 is rescued by Gαq1.hs

      (Hu et al., 2012)
      Partially rescued by

      Gαq961 is partially rescued by GαqH212A.UAS/Scer\GAL4GMR.PF

      (Hu et al., 2012)
      Comments

      Expression of Gα49B1.hs using heat shock rescues the electroretinogram defects of Gα49B961 flies.

      Expression of Gα49BH212A.Scer\UAS under the control of Scer\GAL4GMR.PF rescues deactivation kinetics but not electroretinogram amplitude in Gα49B961 flies.

      (Hu et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Availability

      Stated to be lost.

      (Cao et al., 2018)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Gα49B961
      Gαq961
      (Cao et al., 2018, Hu et al., 2012)
      Gαq961
      (Gu et al., 2020)
      sast
      (Hu et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)