|Feature type||allele||Associated gene||Dmel\Tip60|
|Mutagen||in vitro construct - regulatory fusion, in vitro construct - amino acid replacement, in vitro construct - site directed mutagenesis|
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Controlled Vocabulary Terms
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Scer\UAS regulatory sequences drive expression of a full-length Tip60 ORF sequence with a mutation in the MYST domain.
|Carried in construct|
|Phenotype Manifest In|
Expression of Tip60[E431Q.Scer\UAS] at 25[o]C from late embryonic development to adulthood, under the control of Scer\GAL4[337Y] is lethal. The developmental stage when lethality occurs varies between individual fly lines, with the majority of lethality occurring during the late pupal stage for line A, and during the late second instar larvae stages for lines B and C. Similar results are seen upon expression with Scer\GAL4[Act5C.PI]. Endogenous levels of acetylated histone His4 are significantly depleted in in Scer\GAL4[337Y]-->Tip60[E431Q.Scer\UAS] flies. Expression of Tip60[E431Q.Scer\UAS] in the nervous system, under the control of Scer\GAL4[elav.PLu] (pan-neuronally), Scer\GAL4[179Y] (pan-neuronally) or Scer\GAL4[60IIA] (specifically in the brain and CNS), causes a reduction in viability. This viability varies between 0-25% for expression under the control of Scer\GAL4[elav.PLu] (depending on the transgenic line), 30% for Scer\GAL4[179Y], and 40% for Scer\GAL4[60IIA], respectively.
Pre-synaptic expression of Tip60[E431Q.Scer\UAS] under the control of Scer\GAL4[elav-C155] results in pupal lethality. There is a significant increase in the total number of synaptic boutons in Tip60[E431Q.Scer\UAS]-expressing larvae, when compared to wild-type controls. There is a substantially larger expansion of type-I boutons compared to type-Ib in these mutants. There is also a slight increase in the number of satellite boutons in these mutants compared to controls. This increase in bouton number is accompanied by an excess of futsch-associated microtubule looping and splaying. Neuromuscular junctions in these larvae exhibit a reduction in acetylated microtubule staining, compared to wild-type. Post-synaptic expression of Tip60[E431Q.Scer\UAS] under the control of Scer\GAL4[Mef2.247] results in pupal lethality. Mutant larvae exhibit a significant reduction in bouton number, specifically in 1s boutons, as well as the absence of satellite boutons.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 2 )|