Allele Dmel\Tip60^{E431Q.Scer\UAS}

General Information
Symbol	Dmel\Tip60^{E431Q.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0268425
Feature type	allele	Associated gene	Dmel\Tip60

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement, in vitro construct - site directed mutagenesis
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms in vitro construct - site directed mutagenesis semi-lethal
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Sarthi and Elefant, 2011) in vitro construct - regulatory fusion (Sarthi and Elefant, 2011, Lorbeck et al., 2011) in vitro construct - site directed mutagenesis (Lorbeck et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a full-length Tip60 ORF sequence with a mutation in the MYST domain. (Lorbeck et al., 2011) Amino acid replacement: E431Q. (Sarthi and Elefant, 2011, Lorbeck et al., 2011) Scer\UAS regulatory sequences drive expression of Tip60 coding sequences containing an amino acid mutation. (Sarthi and Elefant, 2011)
Carried in construct	P{UAS-Tip60.E431Q} (Sarthi and Elefant, 2011, Lorbeck et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^337Y (Lorbeck et al., 2011) lethal \| pupal stage, with Scer\GAL4^Act5C.PI (Lorbeck et al., 2011) lethal \| pupal stage, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) lethal \| pupal stage, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) neuroanatomy defective \| larval stage, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) neuroanatomy defective \| larval stage, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) partially lethal - majority die, with Scer\GAL4^60IIA (Lorbeck et al., 2011) partially lethal - majority die, with Scer\GAL4^179Y (Lorbeck et al., 2011) partially lethal - majority die, with Scer\GAL4^elav.PLu (Lorbeck et al., 2011)
Phenotype Manifest In
	terminal bouton, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) terminal bouton, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) type I bouton \| ectopic, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) type I bouton \| ectopic, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) type Ib terminal bouton \| ectopic, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) type Ib terminal bouton \| ectopic, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) type Is terminal bouton, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011)
Detailed Description
	Statement Reference Expression of Tip60[E431Q.Scer\UAS] at 25[o]C from late embryonic development to adulthood, under the control of Scer\GAL4[337Y] is lethal. The developmental stage when lethality occurs varies between individual fly lines, with the majority of lethality occurring during the late pupal stage for line A, and during the late second instar larvae stages for lines B and C. Similar results are seen upon expression with Scer\GAL4[Act5C.PI]. Endogenous levels of acetylated histone His4 are significantly depleted in in Scer\GAL4[337Y]-->Tip60[E431Q.Scer\UAS] flies. Expression of Tip60[E431Q.Scer\UAS] in the nervous system, under the control of Scer\GAL4[elav.PLu] (pan-neuronally), Scer\GAL4[179Y] (pan-neuronally) or Scer\GAL4[60IIA] (specifically in the brain and CNS), causes a reduction in viability. This viability varies between 0-25% for expression under the control of Scer\GAL4[elav.PLu] (depending on the transgenic line), 30% for Scer\GAL4[179Y], and 40% for Scer\GAL4[60IIA], respectively. (Lorbeck et al., 2011) Pre-synaptic expression of Tip60[E431Q.Scer\UAS] under the control of Scer\GAL4[elav-C155] results in pupal lethality. There is a significant increase in the total number of synaptic boutons in Tip60[E431Q.Scer\UAS]-expressing larvae, when compared to wild-type controls. There is a substantially larger expansion of type-I boutons compared to type-Ib in these mutants. There is also a slight increase in the number of satellite boutons in these mutants compared to controls. This increase in bouton number is accompanied by an excess of futsch-associated microtubule looping and splaying. Neuromuscular junctions in these larvae exhibit a reduction in acetylated microtubule staining, compared to wild-type. Post-synaptic expression of Tip60[E431Q.Scer\UAS] under the control of Scer\GAL4[Mef2.247] results in pupal lethality. Mutant larvae exhibit a significant reduction in bouton number, specifically in 1s boutons, as well as the absence of satellite boutons. (Sarthi and Elefant, 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Tip60^{E431Q.Scer\UAS} is rescued by Scer\GAL4^337Y/Tip60^Scer\UAS.cLa (Lorbeck et al., 2011)
Comments	Co-expression of Tip60[Scer\UAS.cLa] rescues the lethality associated with the expression of Tip60[E431Q.Scer\UAS], when both transgenes are under the control of Scer\GAL4[337Y]. (Lorbeck et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Tip60^{E431Q.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Lorbeck et al., 2011, PLoS ONE 6(4): e18412 Microarray analysis uncovers a role for tip60 in nervous system function and general metabolism. [FBrf0213446] Sarthi and Elefant, 2011, PLoS ONE 6(10): e26202 dTip60 HAT Activity Controls Synaptic Bouton Expansion at the Drosophila Neuromuscular Junction. [FBrf0216592]

Allele Dmel\Tip60E431Q.Scer\UAS

Allele Dmel\Tip60^{E431Q.Scer\UAS}