856 bp deletion resulting from the imprecise excision of P{EPgy2}PfasEY05576 extends into Pfas from the insertion site and removes 393 bp of coding sequence. 33 bp of the P-element inverted repeat remain at the site of the insertion.
Mutants die as predominantly at pupal stage P5, with necrotic tissue present in most of the pupae.
Wing discs from homozygous white prepupae contain apoptotic cells.
Pfas1-6 has lethal | pupal stage P5 phenotype, suppressible | partially by BacA\p35UAS.cHa
Pfas1-6 has melanotic necrosis | pupal stage P5 phenotype, suppressible | partially by BacA\p35UAS.cHa
Pfas1-6 has melanotic necrosis | pupal stage P5 phenotype, non-suppressible by p535A-1-4/p53[+]
Pfas1-6 has melanotic necrosis | pupal stage P5 phenotype, non-suppressible by Df(3L)H99/+
BacA\p35Scer\UAS.cHa (in the absence of a Scer\GAL4 driver) partially suppresses the lethality and necrotic phenotypes of ade21-6 animals, with suppression being dose-dependent.
Pfas1-6 is rescued by Scer\GAL4αTub84B.PL/PfasUASp.cHa