Allele Dmel\Eogt^Δ7.4

General Information
Symbol	Dmel\Eogt^Δ7.4	Species	D. melanogaster
Name		FlyBase ID	FBal0268495
Feature type	allele	Associated gene	Dmel\Eogt

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Sakaidani et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference imprecise excision of P{GT1}Eogt[BG00673], located 160bp upstream of the Eogt translation start site, generates a 1.3kb deletion of the Eogt coding region, including the translational start codon, signal peptide, and a portion of the catalytic domain (up to residue F395). (Sakaidani et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage (Sakaidani et al., 2011) lethal \| larval stage (with Eogt^Δ34.2) (Sakaidani et al., 2011)
Phenotype Manifest In
	embryonic/larval cuticle (Sakaidani et al., 2011) embryonic/larval cuticle (with Eogt^LL00272) (Sakaidani et al., 2011) embryonic/larval dorsal trunk (Sakaidani et al., 2011) embryonic/larval dorsal trunk (with Eogt^LL00272) (Sakaidani et al., 2011) embryonic/larval epidermis (with Eogt^LL00272) (Sakaidani et al., 2011) embryonic/larval tracheal system (Sakaidani et al., 2011) embryonic/larval tracheal system (with Eogt^LL00272) (Sakaidani et al., 2011) epithelium (Sakaidani et al., 2011) epithelium (with Eogt^LL00272) (Sakaidani et al., 2011)
Detailed Description
	Statement Reference Eogt[Δ7.4] embryos, lacking both maternal and zygotic Eogt, show a defect in the formation of the innermost layer of the apical extracellular matrix and it's attachment to the epidermis. Eogt[Δ7.4] homozygotes and Eogt[Δ7.4]/Eogt[LL00272] transheterozygotes die mostly during second-instar or second/third instar interface, but some survive until early third-instar. These mutant larvae display cuticle defects and irregular tracheal morphology. Ultrastructural analysis of the larval epidermis reveals disruption of the deposition zone of the endocuticle, leading to separation of the epidermis from the chitin layers. The larval body is not compacted and the body size is comparable to that of wild-type larvae. Eogt[Δ7.4] embryos, lacking both maternal and zygotic Eogt exhibit largely intact tracheal networks, although the tracheal tubes can occasionally be slightly twisted or bent and dorsal trunks are occasionally broken. (Sakaidani et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Eogt^Δ7.4 is rescued by Eogt^Scer\UAS.cSa/Scer\GAL4^αTub84B.PL (Sakaidani et al., 2011)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Eogt^7.4 (Sakaidani et al., 2011) Eogt^Δ7.4
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Sakaidani et al., 2011, Nat. Commun. 2: 583 O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions. [FBrf0217928]

Allele Dmel\EogtΔ7.4

Allele Dmel\Eogt^Δ7.4