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General Information
Symbol
Scer\GAL4ppk25.PS
Species
S. cerevisiae
Name
pickpocket 25 construct of Starostina
FlyBase ID
FBal0268525
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ppk25-Gal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A genomic fragment containing the ppk25 gene, including 5kb from upstream of the ppk25 start codon and approximately 2kb of non-coding downstream sequence. The GAL4 open reading frame has been inserted immediately after the ppk25 start codon. A mutation has been introduced in the start codon of the ppk25 reading frame, to prevent expression of the ppk25 protein, but to allow any potential regulatory sequences that may be present in the intron to be included in the construct.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
RACE
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4ppk25.PS transcript is detected by RLM-RACE in male prothoracic legs, but not in adult heads from which the antennae had been removed. Scer\GAL4ppk25.PS drives expression in a small subset of gustatory and olfactory receptor neurons, some of which have been implicated in male courtship behavior, and which coexpress fru. Expression is observed in a subset of neurons innervating the taste hairs of both male and female legs and wings, but in no other body parts. Scer\GAL4ppk25.PS is limited to one gustatory neuron per leg taste bristle. Expression is also observed in olfactory neurons that project from antennal segment three to antennal lobes VA1v or VL2a. Expression does not overlap with that of CheB42a protein.

Scer\GAL4ppk25.PS drives expression in one of four gustatory receptor neurons of many of the taste bristles in the male legs; expression is consistantly observed in 43 gustatory receptor neurons on the tibia and five tarsal segments. All of the prothoracic gustatory receptor neurons labelled by Scer\GAL4ppk25.PS co-express Ecol\lexAfru.P1. For the pairs of prothoracic neurons labelled by Ecol\lexAfru.P1, expression of Scer\GAL4ppk25.PS can be detected in both, or only one of the neurons. Expression of Scer\GAL4ppk25.PS and Scer\GAL4ppk25.PS is very similar: all but 11 Scer\GAL4ppk29.3.020 positive neurons, are also labelled by Scer\GAL4ppk29.3.020. In most cases, these 11 Scer\GAL4ppk29.3.020-expressing neurons are paired within a single taste hair with a neuron that expresses both Scer\GAL4ppk25.PS and Scer\GAL4ppk29.3.020. Gustatory receptor neurons in the wing margin, but not in the labellum, also express Scer\GAL4ppk25.PS. The leg and wing neurons project to each of the thoracic ganglia. The prothoracic neurons that are labelled by Scer\GAL4ppk25.PS send sexually dimorphic projections to the first thoracic ganglion: projections from males cross the midline more often than female ones.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of Scer\GAL4ppk25.PS cells in adult males significantly increases wing extensions towards females, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4ppk25.PS
Name Synonyms
pickpocket 25 construct of Starostina
Secondary FlyBase IDs
    References (4)