Open Close
General Information
Symbol
Dmel\Vha13cnj-356
Species
D. melanogaster
Name
FlyBase ID
FBal0268627
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 1 base deletion that creates a frameshift predicted to encode a 136 amino acid protein, 91 amino acids of which are novel.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A deletion that creates a frameshift predicted to encode a larger than wild type protein, all bu 45 residues of which are novel.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant tracheal terminal cells show a gas-filling defect at the stalk cell to terminal cell connection - most gaps are present in heterozygous stalk cell tubes adjacent to Vha13cnj-356 mutant cell clones. The defects are 100% penetrant with late onset (third larval instar). The stalk cell-terminal cell interface is also affected: 25% of Vha13cnj-356 cells exhibit stalk cells aberrantly forming two or more connections to a neighbouring terminal cell, and 8% of Vha13cnj-356 cells show a more severe defect in which long, branched autocellular, seamed tubes extend well beyond the terminal cell nucleus. These ectopic, seamed tubes derive from the adjacent stalk cell.

Vha13cnj-356 dorsal trunk clones are not fully integrated into the epithelium - cells are oval (not hexagonal) in shape and have a significant decrease in the amount of apical membrane. Vha13cnj-356 stalk cell clones have abnormal autocellular and intercellular junctions - these cells are detached from the epithelium or contribute a very short segment of autocellular tube.

Tubulogenesis in Vha13cnj-356 embryos is intact, and tube defects are only observed in clones later in larval development - ie. tube architecture only becomes disrupted over time as the the tracheal system increases in size.

conjoined356 mutants exhibit thick and severely pruned tracheal terminal cells. There is also defective intercalation/auto-cellular tube formation. They also exhibit an auto-/sub-cellular tube gas-filling defect. Dorsal trunk cells are rounded and contribute minimally to multicellular lumen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Scer\GAL4btl.PU-mediated expression of Vha13Scer\UAS.cFa rescues the tracheal defects associated with Vha13cnj-356.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)