FB2021_02, released April 13, 2021

Allele: Dmel\Vha13cnj-356

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General Information
Symbol
Dmel\Vha13cnj-356
Species
D. melanogaster
Name
FlyBase ID
FBal0268627
Feature type
allele
Associated gene
Dmel\Vha13
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Francis and Ghabrial, 2015)
Mutagen
ethyl methanesulfonate
(Ghabrial et al., 2011)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
3R:19,643,784..19,643,784 [-]
Comment:

A 1 base deletion that creates a frameshift predicted to encode a 136 amino acid protein, 91 amino acids of which are novel.

(Francis and Ghabrial, 2015)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      A deletion that creates a frameshift predicted to encode a larger than wild type protein, all bu 45 residues of which are novel.

      (Francis and Ghabrial, 2015)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant tracheal terminal cells show a gas-filling defect at the stalk cell to terminal cell connection - most gaps are present in heterozygous stalk cell tubes adjacent to Vha13cnj-356 mutant cell clones. The defects are 100% penetrant with late onset (third larval instar). The stalk cell-terminal cell interface is also affected: 25% of Vha13cnj-356 cells exhibit stalk cells aberrantly forming two or more connections to a neighbouring terminal cell, and 8% of Vha13cnj-356 cells show a more severe defect in which long, branched autocellular, seamed tubes extend well beyond the terminal cell nucleus. These ectopic, seamed tubes derive from the adjacent stalk cell.

      Vha13cnj-356 dorsal trunk clones are not fully integrated into the epithelium - cells are oval (not hexagonal) in shape and have a significant decrease in the amount of apical membrane. Vha13cnj-356 stalk cell clones have abnormal autocellular and intercellular junctions - these cells are detached from the epithelium or contribute a very short segment of autocellular tube.

      Tubulogenesis in Vha13cnj-356 embryos is intact, and tube defects are only observed in clones later in larval development - ie. tube architecture only becomes disrupted over time as the the tracheal system increases in size.

      (Francis and Ghabrial, 2015)

      conjoined356 mutants exhibit thick and severely pruned tracheal terminal cells. There is also defective intercalation/auto-cellular tube formation. They also exhibit an auto-/sub-cellular tube gas-filling defect. Dorsal trunk cells are rounded and contribute minimally to multicellular lumen.

      (Ghabrial et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Vha13CA07644

      (Francis and Ghabrial, 2015)
      Rescued by

      Vha13cnj-356 is rescued by Scer\GAL4btl.PU/Vha13UAS.cFa

      (Francis and Ghabrial, 2015)
      Comments

      Scer\GAL4btl.PU-mediated expression of Vha13Scer\UAS.cFa rescues the tracheal defects associated with Vha13cnj-356.

      (Francis and Ghabrial, 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      356
      (Ghabrial et al., 2011)
      Vha13cnj-356
      (Nagata et al., 2019)
      cnj356
      (Francis and Ghabrial, 2015)
      conjoined356
      Name Synonyms
      Secondary FlyBase IDs
        References (3)