Co-expression of bansponge.Scer\UAS.T:Disc\RFP, but not banD.Scer\UAS.T:Avic\GFP-EGFP, suppresses the ectopic type II neuroblast phenotype seen in larval brain clones expressing numbTS4D.Scer\UAS under the control of Scer\GAL4Act.PU.
Expression of NcΔN.Scer\UAS attenuates numbTS4D.Scer\UAS induced ectopic neuroblast formation. No change in apoptosis is reported in this background, although the number of postmitotic neurons is increased.
Co-expression of ponS611D.Scer\UAS under the control of Scer\GAL4insc-Mz1407 rescues numbTS4D.Scer\UAS localisation at metaphase and telophase, but is unable to rescue ectopic type II neuroblast formation.
Co-expression of p53H159N.Scer\UAS.Ex (that lacks transactivation activity) does not suppress supernumerary neuroblast formation induced by numbTS4D.Scer\UAS expression under the control of Scer\GAL4insc-Mz1407.
Larval brains expressing numbTS4D.Scer\UAS and p53Scer\UAS.Ex under the control of Scer\GAL4insc-Mz1407 exhibit an overall increase in apoptosis compared to controls (as visualised by TUNEL). However, neuroblast apoptosis remains rare, indicating that the apoptosis increase is in post-mitotic neurons or other brain cells.
A dapunspecified mutant background does not attenuate the ability of p53Scer\UAS.Ex expression to suppress the formation of ectopic neuroblasts induced by numbTS4D.Scer\UAS expression (under the control of Scer\GAL4insc-Mz1407).