FlyBase Allele Report: Dmel\numb[TS4D.UAS]

General Information

Symbol

Dmel\numb^TS4D.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0268717

Feature type

allele

Associated gene

Dmel\numb

Associated Insertion(s)

Carried in Construct

P{UAS-numb.TS4D}

Also Known As

Numb-TS4D

Key Links

Transgenic product class

missense_variant

(Ouyang et al., 2011)

polypeptide_post_translational_processing_variant

(Ouyang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ouyang et al., 2011)

polypeptide_post_translational_processing_variant

(Ouyang et al., 2011)

Mutagen

in vitro construct

(Ouyang et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-numb.TS4D}

Cytology

Description

UAS regulatory sequences drive expression of numb sequences where five putative phospho-sites (T143, S183, S188, S248 and S447) have been mutated to be phosphomimetic (i.e. appear always phosphorylated).

(Ouyang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

numb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

abnormal neuroanatomy, with Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

abnormal neuroanatomy | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Wu et al., 2017)

increased cell number | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Wu et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Larval brain clones expressing numb^{TS4D.Scer\UAS} under the control of Scer\GAL4^Act.PU exhibit ectopic type II neuroblasts.

(Wu et al., 2017)

Overexpression of numb^{TS4D.Scer\UAS} in larval brain neuroblasts under the control of Scer\GAL4^insc-Mz1407 leads to a very dramatic increase in type II neuroblast number (compared to expression of numb^S2D.Scer\UAS). While neuroblasts are more proliferative in these mutants, the number of elav-positive postmitotic neurons. numb localisation into basal crescents in these neuroblasts appears normal, although some show compromised localisation at metaphase and telophase.

Expression of numb^{TS4D.Scer\UAS} in the type-I neuroblast lineage under the control of Scer\GAL4^ase.neuro has no effect on neuroblast number.

Scer\GAL4^erm.R9D11-driven expression of numb^{TS4D.Scer\UAS} in mature INPs, GMCs, and differentiated neurons fails to induce ectopic neuroblast formation.

Type-II neuroblasts clones expressing numb^{TS4D.Scer\UAS} contain ectopic neuroblasts, whereas numb^{TS4D.Scer\UAS}-expressing type-I neuroblast clones always contain a single neuroblast, as found in controls.

(Ouyang et al., 2011)

Expression of numb^{TS4D.Scer\UAS} under the control of Scer\GAL4^insc-Mz1407 induces supernumerary neuroblast formation.

(Ouyang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS

(Ouyang et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, numb^TS4D.UAS has increased cell number | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

Scer\GAL4^Act.PU, numb^TS4D.UAS has abnormal neuroanatomy | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, suppressible by spdo^G104

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, suppressible by N^l1N-ts1

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, suppressible by N^GD144, Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act.PU, numb^TS4D.UAS has increased cell number | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/mir-ban^D.UAS.EGFP

(Wu et al., 2017)

Scer\GAL4^Act.PU, numb^TS4D.UAS has abnormal neuroanatomy | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/mir-ban^D.UAS.EGFP

(Wu et al., 2017)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/pon^S611D.UAS

(Ouyang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Other

Statement

Reference

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS, p53^UAS.Ex has increased cell death phenotype

(Ouyang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has neuron | increased number phenotype, enhanceable by Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS

(Ouyang et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, numb^TS4D.UAS has type II neuroblast | increased number | somatic clone | larval stage phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/mir-ban^{sponge.UAS.DsRed(Unk)}

(Wu et al., 2017)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has type II neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407/Dronc^ΔN.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has type II neuroblast | increased number phenotype, suppressible by spdo^G104

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has type II neuroblast | increased number phenotype, suppressible by N^l1N-ts1

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has type II neuroblast | increased number phenotype, suppressible by N^GD144, Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has type II neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407/p53^UAS.Ex

(Ouyang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act.PU, numb^TS4D.UAS has type II neuroblast | increased number | somatic clone | larval stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/mir-ban^D.UAS.EGFP

(Wu et al., 2017)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has type II neuroblast | increased number phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/pon^S611D.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS, p53^UAS.Ex has neuroblast phenotype, non-suppressible by dap^unspecified

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS has neuroblast | increased number phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/p53^H159N.UAS.Ex

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS, p53^UAS.Ex has neuroblast phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS is a suppressor of type II neuroblast | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ban^{sponge.Scer\UAS.T:Disc\RFP}, but not ban^{D.Scer\UAS.T:Avic\GFP-EGFP}, suppresses the ectopic type II neuroblast phenotype seen in larval brain clones expressing numb^{TS4D.Scer\UAS} under the control of Scer\GAL4^Act.PU.

(Wu et al., 2017)

Expression of Nc^pro.Scer\UAS attenuates numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation.

Expression of Nc^ΔN.Scer\UAS attenuates numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation. No change in apoptosis is reported in this background, although the number of postmitotic neurons is increased.

Co-expression of pon^{S611D.Scer\UAS} under the control of Scer\GAL4^insc-Mz1407 rescues numb^{TS4D.Scer\UAS} localisation at metaphase and telophase, but is unable to rescue ectopic type II neuroblast formation.

numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation is abolished in spdo^G104 mutant clones.

Inhibition of N activity through N^l1N-ts1 suppresses numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation.

Inhibition of N activity through N^GD144 suppresses numb^{TS4D.Scer\UAS} induced ectopic neuroblast formation.

(Ouyang et al., 2011)

Co-expression of p53^Scer\UAS.Ex suppresses ectopic neuroblast formation induced by numb^{TS4D.Scer\UAS} expression under the control of Scer\GAL4^insc-Mz1407.

Co-expression of p53^{H159N.Scer\UAS.Ex} (that lacks transactivation activity) does not suppress supernumerary neuroblast formation induced by numb^{TS4D.Scer\UAS} expression under the control of Scer\GAL4^insc-Mz1407.

Larval brains expressing numb^{TS4D.Scer\UAS} and p53^Scer\UAS.Ex under the control of Scer\GAL4^insc-Mz1407 exhibit an overall increase in apoptosis compared to controls (as visualised by TUNEL). However, neuroblast apoptosis remains rare, indicating that the apoptosis increase is in post-mitotic neurons or other brain cells.

A dap^unspecified mutant background does not attenuate the ability of p53^Scer\UAS.Ex expression to suppress the formation of ectopic neuroblasts induced by numb^{TS4D.Scer\UAS} expression (under the control of Scer\GAL4^insc-Mz1407).

(Ouyang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa does not affect the suppression of ectopic neuroblast formation by p53^Scer\UAS.Ex in numb^{TS4D.Scer\UAS}-expressing larval brains (all transgenes under the control of Scer\GAL4^insc-Mz1407).

(Ouyang et al., 2011)

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^insc-Mz1407/numb^TS4D.UAS fails to rescue numb¹⁵

(Ouyang et al., 2011)

Comments

Expression of numb^{TS4D.Scer\UAS} fails to suppress the ectopic neuroblast formation seen in numb¹⁵ mutant clones.

(Ouyang et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

numb^{TS4D.Scer\UAS}

numb^TS4D.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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