FlyBase Allele Report: Dmel\Neto[36]

General Information

Symbol

Dmel\Neto³⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0268739

Feature type

allele

Associated gene

Dmel\Neto

Associated Insertion(s)

Carried in Construct

Also Known As

neto^null

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

transposable element activity

Nature of the Allele

Allele class

Mutagen

transposable element activity

(Kim et al., 2012)

Progenitor genotype

Mi{ET1}Neto^MB04917

(Kim et al., 2012)

Cytology

Description

Imprecise excision of Mi{ET1}Neto^MB04917, resulting in removal of sequence X: 13391744-13394080, including exon 5 of the predicted Neto gene. This deletion introduces frameshifts and early stop codons, and the Neto ORF is truncated within the first CUB domain.

(Kim et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:13,497,711..13,500,047 [+]

Comment:

2337 bp deletion resulting from the imprecise excision of Mi{ET1}Neto^MB04917, which includes exons 5.

(Kim et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction, with Neto^UAS.EGFP, Scer\GAL4^G14

(Kim et al., 2015)

embryonic/larval neuromuscular junction, with Neto^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^G14

(Kim et al., 2015)

terminal bouton, with Neto^PM.UAS.GFP, Scer\GAL4^G14

(Kim et al., 2015)

terminal bouton, with Neto^UAS.cSa, Scer\GAL4^G14

(Kim et al., 2015)

Detailed Description

Statement

Reference

Expression of Neto^Scer\UAS.cSa in a wild type background under the control of Scer\GAL4^G14 results in a reduction in NMJ bouton number and shorter branches. Expression in a mutant background (Neto³⁶) also results in a dose-dependent reduction in bouton number and branching: the higher the expression level of the transgene the more severe the phenotype. Overexpression of Neto also has detrimental effects on the viability of rescued animals at all stages of development. There is a reduction in mEJP frequency compared to controls and a small reduction in amplitude when Neto^Scer\UAS.cSa is expressed at very high levels. EJP amplitude is also reduced at high expression levels.

Expression of Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^G14 in a Neto³⁶ mutant background results in smaller NMJs with normal synaptic transmission.

Expression of Neto^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^G14 in a Neto³⁶ mutant background results in smaller NMJs with normal synaptic transmission.

(Kim et al., 2015)

Neto³⁶ hemizygous embryos have normal overall appearance and completely fill their trachea with gas, but they never hatch into larval stages and they die within 2 days after egg laying. This phenotype is 100% penetrant. Mutant embryos are completely paralysed and lack any body wall peristalsis and hatching movements. Manually hatched animals fail to move or feed.

(Kim et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Neto[+]/Neto³⁶ is a non-enhancer of abnormal neuroanatomy phenotype of gbb²/gbb¹

(Sulkowski et al., 2014)

Other

Statement

Reference

Neto³⁶, gbb¹/gbb[+] has abnormal neuroanatomy phenotype

(Sulkowski et al., 2014)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Neto[+]/Neto³⁶ is a non-enhancer of terminal bouton phenotype of gbb²/gbb¹

(Sulkowski et al., 2014)

Other

Statement

Reference

Neto³⁶, gbb¹/gbb[+] has embryonic/larval neuromuscular junction phenotype

(Sulkowski et al., 2014)

Neto³⁶, gbb¹/gbb[+] has terminal bouton phenotype

(Sulkowski et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae that are heterozygous for both Neto³⁶ and gbb¹ have smaller NMJs with 30% fewer synaptic boutons than controls.

Larvae that are heterozygous for both Neto³⁶ and wit^B11 have a similar number of boutons to wild type.

Larvae that are heterozygous for both Neto³⁶ and put⁶² have a similar number of boutons to wild type.

Larvae that are heterozygous for Neto³⁶, wit^G15 and put⁶² have smaller NMJs with 25% fewer synaptic boutons.

One copy of Neto³⁶ does not enhance the reduction in NMJ bouton number seen in gbb¹/gbb² mutant larvae.

(Sulkowski et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Neto³⁶ is rescued by Scer\GAL4^G14/Neto^UAS.EGFP

(Kim et al., 2015)

Neto³⁶ is rescued by Scer\GAL4^G14/Neto^UAS.cSa

(Ramos et al., 2015)

Neto³⁶ is rescued by Scer\GAL4^G14/Neto^UAS.β

(Ramos et al., 2015)

Neto³⁶ is rescued by Scer\GAL4^G14/Neto^{Δintra.UAS.EGFP}

(Ramos et al., 2015)

Neto³⁶ is rescued by Scer\GAL4^G14/Neto^UAS.EGFP

(Kim et al., 2012)

Partially rescued by

Neto³⁶ is partially rescued by Scer\GAL4^G14/Neto^CA.UAS.GFP

(Kim et al., 2015)

Neto³⁶ is partially rescued by Scer\GAL4^G14/Neto^PM.UAS.GFP

(Kim et al., 2015)

Neto³⁶ is partially rescued by Scer\GAL4^G14/Neto^UAS.EGFP

(Kim et al., 2012)

Neto³⁶ is partially rescued by Neto^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^da.G32

(Kim et al., 2012)

Neto³⁶ is partially rescued by Neto^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^how-24B

(Kim et al., 2012)

Neto³⁶ is partially rescued by Scer\GAL4^G14/Neto^{UAS.Tag:V5,Tag:polyHis}

(Kim et al., 2012)

Not rescued by

Neto³⁶ is not rescued by Neto^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^elav.PLu

(Kim et al., 2012)

Neto³⁶ is not rescued by Neto^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^btl.PS

(Kim et al., 2012)

Comments

Expression of Neto^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^G14 largely rescues the embryonic lethality and adult viability of Neto³⁶ mutants.

Expression of Neto^{PM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^G14 is unable to rescue the embryonic lethality and adult viability of Neto³⁶ mutants. Very few animals reach the adult stages and the larvae die during the larval stages. They do not fly and have locomotor defects. The NMJ morphology defects seen in Neto³⁶ are not rescued at moderate levels of expression, and at high expression levels the NMJs are severely altered. The postsynaptic markers are severely disrupted in rescued animals but these post-synaptic defects are not accompanied by cytoskeletal disruption. Rare boutons are seen with no postsynaptic densities (PSDs) and no detectable subsynaptic reticulum (SSR), surrounded instead by dense ribosome fields or myofibrils. The T-bar structures are increased in diameter and are often mis-shaped, collapsed or floating at boutons and there is an increase in synaptic vesicle size. The spontaneous miniature excitatory junctional potential (mEJP) frequency and amplitude are reduced compared to controls. Evoked EJPs are normal. Presynaptic markers are unaffected.

Expression of Neto^{CA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^G14 largely rescues the embryonic lethality and adult viability of Neto³⁶ mutants. The NMJ morphology defects are also rescued at low levels of expression although bouton number is still reduced at high expression levels.

(Kim et al., 2015)

Expression of Neto^Scer\UAS.cSa in muscles under the control of Scer\GAL4^G14 rescues the embryonic lethality and paralysis seen in Neto³⁶ mutants. Neto³⁶ mutant flies expressing low/moderate levels of Scer\GAL4^G14 under the control of Scer\GAL4^G14 have significantly reduced spontaneous miniature excitatory junctional potential (mEJP) amplitudes. mEJP frequency is largely normal. Quantal content is significantly increased and evoked EJP is normal.

Expression of Neto^Scer\UAS.β in muscles under the control of Scer\GAL4^G14 rescues the embryonic lethality and paralysis seen in Neto³⁶ mutants. Neto³⁶ mutant flies expressing low/moderate levels of Scer\GAL4^G14 under the control of Scer\GAL4^G14 have relatively normal spontaneous miniature excitatory junctional potential (mEJP) amplitude and frequency, although increased frequency is seen at 18[o]C. Evoked EJP is normal.

Expression of Neto^{Δintra.Scer\UAS.T:Avic\GFP-EGFP} in muscles under the control of Scer\GAL4^G14 rescues the embryonic lethality and paralysis seen in Neto³⁶ mutants.

Dp(1;3)DC270 rescues the lethality seen in Neto³⁶/Neto²⁰⁴ mutant flies.

(Ramos et al., 2015)

The lethality of Neto³⁶ embryos is rescued to larval stages by expression of Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} using Scer\GAL4^da.G32,

Scer\GAL4^how-24B or Scer\GAL4^G14. Moreover, a number of Neto³⁶ animals are rescued all the way to viable and fertile adults using Scer\GAL4^how-24B- or Scer\GAL4^G14-mediated expression of Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}.

The lethality of Neto³⁶ is completely rescued by Dp(1;3)DC270.

Scer\GAL4^G14-mediated expression of Neto^{Scer\UAS.T:Avic\GFP-EGFP} rescues Neto³⁶ mutants to adulthood when the temperature is maintained at 25[o]C. However, if the temperature is reduced to 18[o]C (to reduce Scer\GAL4 expression/activity) when first instar larvae hatch, most animals die at larval-pupal stages and exhibit altered, smaller synapses at third instar larval stage.

(Kim et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Neto³⁶

neto³⁶

(Kim et al., 2015, Ramos et al., 2015, Sulkowski et al., 2014, Kim et al., 2012)

neto^null

(Han et al., 2020, Ramos et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (5)

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