Allele Dmel\Neto³⁶

General Information
Symbol	Dmel\Neto³⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0268739
Feature type	allele	Associated gene	Dmel\Neto

Allele class
Mutagen	transposable element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	transposable element activity (Kim et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Mi{ET1}Neto^MB04917 (Kim et al., 2012)
Nature of the lesion	Statement Reference Imprecise excision of Mi{ET1}Neto[MB04917], resulting in removal of sequence X: 13391744-13394080, including exon 5 of the predicted Neto gene. This deletion introduces frameshifts and early stop codons, and the Neto ORF is truncated within the first CUB domain. (Kim et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	hatching behavior defective (Kim et al., 2012) lethal \| embryonic stage (Kim et al., 2012)
Phenotype Manifest In

Detailed Description
	Statement Reference Neto[36] hemizygous embryos have normal overall appearance and completely fill their trachea with gas, but they never hatch into larval stages and they die within 2 days after egg laying. This phenotype is 100% penetrant. Mutant embryos are completely paralysed and lack any body wall peristalsis and hatching movements. Manually hatched animals fail to move or feed. (Kim et al., 2012)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Neto³⁶ is rescued by Scer\GAL4^G14/Neto^{Scer\UAS.T:Avic\GFP-EGFP} (Kim et al., 2012)
Partially rescued by	Neto³⁶ is partially rescued by Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}/Scer\GAL4^da.G32 (Kim et al., 2012) Neto³⁶ is partially rescued by Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}/Scer\GAL4^how-24B/Scer\GAL4^how-24B (Kim et al., 2012) Neto³⁶ is partially rescued by Scer\GAL4^G14/Neto^{Scer\UAS.T:Avic\GFP-EGFP} (Kim et al., 2012) Neto³⁶ is partially rescued by Scer\GAL4^G14/Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} (Kim et al., 2012)
Not rescued by	Neto³⁶ is not rescued by Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}/Scer\GAL4^btl.PS (Kim et al., 2012) Neto³⁶ is not rescued by Neto^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}/Scer\GAL4^elav.PLu (Kim et al., 2012)
Comments	The lethality of Neto[36] embryos is rescued to larval stages by expression of Neto[Scer\UAS.T:SV5\V5,T:Zzzz\His6] using Scer\GAL4[da.G32], Scer\GAL4[how-24B] or Scer\GAL4[G14]. Moreover, a number of Neto[36] animals are rescued all the way to viable and fertile adults using Scer\GAL4[how-24B]- or Scer\GAL4[G14]-mediated expression of Neto[Scer\UAS.T:SV5\V5,T:Zzzz\His6]. The lethality of Neto[36] is completely rescued by Dp(1;3)DC270. Scer\GAL4[G14]-mediated expression of Neto[Scer\UAS.T:Avic\GFP-EGFP] rescues Neto[36] mutants to adulthood when the temperature is maintained at 25[o]C. However, if the temperature is reduced to 18[o]C (to reduce Scer\GAL4 expression/activity) when first instar larvae hatch, most animals die at larval-pupal stages and exhibit altered, smaller synapses at third instar larval stage. (Kim et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Neto³⁶ neto³⁶ (Kim et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Kim et al., 2012, Genes Dev. 26(9): 974--987 Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction. [FBrf0218161]

Allele Dmel\Neto36

Allele Dmel\Neto³⁶