FB2025_01 , released February 20, 2025
Allele: Dmel\NetoUAS.Tag:V5,Tag:polyHis
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General Information
Symbol
Dmel\NetoUAS.Tag:V5,Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0268744
Feature type
allele
Associated gene
Dmel\Neto
Associated Insertion(s)
Carried in Construct
P{UAS-Neto.V5-His}
Key Links
Transgenic product class
cDNA
(Kim et al., 2012)
wild_type
(Kim et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Kim et al., 2012)
wild_type
(Kim et al., 2012)
Mutagen
in vitro construct
(Kim et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-Neto.V5-His}
Cytology
Description

UAS drives expression of the full-length Neto cDNA (from GH11189) tagged at the C-terminus with Tag:V5 and Tag:polyHis.

(Kim et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Neto
Tagged with
Tag:polyHis
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of NetoScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4G14 in a Neto36 mutant background results in smaller NMJs with normal synaptic transmission.

      (Kim et al., 2015)

      Neuromuscular junction synapses in larvae expressing NetoScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4how-24B are reduced in growth.

      (Kim et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      NetoUAS.Tag:V5,Tag:polyHis/Scer\GAL4da.G32 partially rescues Neto36

      (Kim et al., 2012)

      NetoUAS.Tag:V5,Tag:polyHis/Scer\GAL4how-24B partially rescues Neto36

      (Kim et al., 2012)

      Scer\GAL4G14/NetoUAS.Tag:V5,Tag:polyHis partially rescues Neto36

      (Kim et al., 2012)
      Fails to rescue

      NetoUAS.Tag:V5,Tag:polyHis/Scer\GAL4elav.PLu fails to rescue Neto36

      (Kim et al., 2012)

      NetoUAS.Tag:V5,Tag:polyHis/Scer\GAL4btl.PS fails to rescue Neto36

      (Kim et al., 2012)
      Comments

      The lethality of Neto36 embryos is rescued to larval stages by expression of NetoScer\UAS.T:SV5\V5,T:Zzzz\His6 using Scer\GAL4da.G32,

      Scer\GAL4how-24B or Scer\GAL4G14. Moreover, a number of Neto36 animals are rescued all the way to viable and fertile adults using Scer\GAL4how-24B- or Scer\GAL4G14-mediated expression of NetoScer\UAS.T:SV5\V5,T:Zzzz\His6.

      (Kim et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      NetoScer\UAS.T:SV5\V5,T:Zzzz\His6
      NetoUAS.Tag:V5,Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (3)