FB2025_05 , released December 11, 2025
Allele: Dmel\trpRNAi.PG
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General Information
Symbol
Dmel\trpRNAi.PG
Species
D. melanogaster
Name
FlyBase ID
FBal0268979
Feature type
allele
Associated gene
Dmel\trp
Associated Insertion(s)
Carried in Construct
P{trp5'-RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Geng et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Geng et al., 2004)
Mutagen
in vitro construct
(Geng et al., 2004)
Progenitor genotype
Carried in construct
P{trp5'-RNAi}
Cytology
Description

The 5' portion of the trp gene that contains the first intron (834bp) is ligated tail-to-tail with the corresponding 5' portion of trp cDNA (826bp). The native upstream regulatory region of the trp gene is placed at the two ends of the RNAi construct in opposite orientations so that transcription can start from both ends.

(Geng et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
trp
Encoded product / tool
trp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies carrying two copies of the trpdsRNA.PG transgene experiencing a 4 second light stimulus exhibit ERG waveforms that are indistinguishable from that of wild-type flies. After a 30 second bright stimulus these mutants exhibit a small but noticeable decay in photoreceptor potential. Four copies of trpdsRNA.PG generate a futher reduction in trp protein levels and a further decay of the ERG.

      Flies carrying two copies of the trpdsRNA.PG transgene exhibit delayed development or misalignment of rhabdomeres at an early age.

      (Geng et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      trpRNAi.PG rescues trpP365

      (Geng et al., 2004)
      Comments

      The presence of trpdsRNA.PG in a trpP365 heterozygous background rescues rhabdomere organisation and integrity at least up to 28 days post-eclosion. Photoreceptor degeneration is completely rescued through expression of trpdsRNA.PG, as are ERG amplitudes. R1-R6 photoreceptors, which are pereviously non-functional, are now functional and contribute to the near normal ERG amplitudes and to the generation of the transients. However, the termination time course of the responses at stimulus offset was still slower than in wild-type, indicating that rescue is not complete.

      (Geng et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      trpRNAi.PG
      trpdsRNA.PG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)