Allele Dmel\uif^{asterisk.Scer\UAS}

General Information
Symbol	Dmel\uif^{asterisk.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0268981
Feature type	allele	Associated gene	Dmel\uif

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion, in vitro construct - site directed mutagenesis
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Xie et al., 2012) in vitro construct - regulatory fusion (Xie et al., 2012) in vitro construct - site directed mutagenesis (Xie et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a polypeptide that lacks the last ten amino acids of at the C-terminus of the predicted full-length uif protein (amino acid 3548 to amino acid 3557), with two amino acid substitutions (N1567D and A3134T) and an addition of three extra amino acids (SGR) immediately immediately after amino acid 165 resulting from the insertion of a restriction enzyme Not I site in the coding sequence. Compared with wild-type uif, the protein encoded by the construct has an altered intracellular domain. (Xie et al., 2012)
Carried in construct	P{UAS-Uif.*} (Xie et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	partially lethal - majority die, with Scer\GAL4^unspecified (Xie et al., 2012) visible, with Scer\GAL4^A9 (Xie et al., 2012) visible, with Scer\GAL4^Bx-MS1096 (Xie et al., 2012) visible, with Scer\GAL4^dpp.blk1 (Xie et al., 2012) visible, with Scer\GAL4^en-e16E (Xie et al., 2012) visible, with Scer\GAL4^Eq1 (Xie et al., 2012) visible, with Scer\GAL4^GMR.PU (Xie et al., 2012) visible, with Scer\GAL4^pnr-MD237 (Xie et al., 2012)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PU (Xie et al., 2012) notal microchaeta, with Scer\GAL4^Eq1 (Xie et al., 2012) notal microchaeta, with Scer\GAL4^pnr-MD237 (Xie et al., 2012) wing margin, with Scer\GAL4^dpp.blk1 (Xie et al., 2012) wing margin, with Scer\GAL4^en-e16E (Xie et al., 2012) wing vein, with Scer\GAL4^A9 (Xie et al., 2012) wing vein, with Scer\GAL4^Bx-MS1096 (Xie et al., 2012)
Detailed Description
	Statement Reference Ubiquitous expression of uif[asterisk.Scer\UAS] causes a semi-lethal phenotype. Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[en-e16E] results in significant loss of wing margin structures. Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[dpp.blk1] results in wing notches in the distal region. Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] leads to thickened wing veins. Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[Bx-MS1096] leads to thickened wing veins. Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[Eq1] causes sensory mother cell (SOP) selection and formation defects including patches of bristle loss. Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[pnr-MD237] results in the nearly complete loss of bristles in the notum and scutellum Expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[GMR.PU] results in a rough and small eye phenotype. (Xie et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has visible phenotype, enhanceable by Dl^9P/Dl[+] (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has visible phenotype, enhanceable by dx¹/dx[+] (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has visible phenotype, enhanceable by kuz[+]/kuz^e29-4 (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has visible phenotype, enhanceable by N[+]/N^55e11 (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has visible phenotype, enhanceable by N¹/N[+] (Xie et al., 2012)
Suppressed by
Statement Reference Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has visible phenotype, suppressible by E(spl)mβ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^A9 (Xie et al., 2012) Scer\GAL4^GMR.PU, uif^{asterisk.Scer\UAS} has visible phenotype, suppressible \| partially by E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^GMR.PU (Xie et al., 2012)
Enhancer of
Statement Reference uif^{asterisk.Scer\UAS}, Scer\GAL4^sca-109-68 is an enhancer of visible phenotype of Dl^D.Scer\UAS, Scer\GAL4^sca-109-68 (Xie et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has wing vein phenotype, enhanceable by Dl^9P/Dl[+] (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has wing vein phenotype, enhanceable by dx¹/dx[+] (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has wing vein phenotype, enhanceable by kuz[+]/kuz^e29-4 (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has wing vein phenotype, enhanceable by N[+]/N^55e11 (Xie et al., 2012) Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has wing vein phenotype, enhanceable by N¹/N[+] (Xie et al., 2012)
Suppressed by
Statement Reference Scer\GAL4^A9, uif^{asterisk.Scer\UAS} has wing vein phenotype, suppressible by E(spl)mβ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^A9 (Xie et al., 2012) Scer\GAL4^GMR.PU, uif^{asterisk.Scer\UAS} has eye phenotype, suppressible \| partially by E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^GMR.PU (Xie et al., 2012)
Enhancer of
Statement Reference uif^{asterisk.Scer\UAS}, Scer\GAL4^sca-109-68 is an enhancer of scutellar bristle \| supernumerary phenotype of Dl^D.Scer\UAS, Scer\GAL4^sca-109-68 (Xie et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference The wing vein phenotype resulting from the expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] is enhanced by N[1]/+. The wing vein phenotype resulting from the expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] is enhanced by N[55e11]/+. The wing vein phenotype resulting from the expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] is enhanced by Dl[9P]. The wing vein phenotype resulting from the expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] is enhanced by kuz[e29-4]. The wing vein phenotype resulting from the expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] is enhanced by dx[1]. The scutellar bristle phenotype resulting from the expression of Dl[D.Scer\UAS] under the control of Scer\GAL4[sca-109-68] is enhanced by co-expression of uif[asterisk.Scer\UAS]. The thickened wing vein phenotype resulting from the expression of uif[asterisk.Scer\UAS] under the control of Scer\GAL4[A9] is almost completely suppressed by co-expression of E(spl)mβ-HLH[Scer\UAS.cdCa]. The rough and small eye phenotype resulting from the expression of uif[asterisk.Scer\UAS] is significantly alleviated by co-expression of E(spl)m7-HLH[Scer\UAS.cdCa]. (Xie et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	uif^{asterisk.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Xie et al., 2012, PLoS ONE 7(4): e36362 Uif, a Large Transmembrane Protein with EGF-Like Repeats, Can Antagonize Notch Signaling in Drosophila. [FBrf0218242]

Allele Dmel\uifasterisk.Scer\UAS

Allele Dmel\uif^{asterisk.Scer\UAS}