Allele Dmel\Mad^{Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Mad^{Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0269039
Feature type	allele	Associated gene	Dmel\Mad

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Oh and Irvine, 2011) in vitro construct - regulatory fusion (Oh and Irvine, 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Mad tagged with T:Zzzz\FLAG. (Oh and Irvine, 2011)
Carried in construct	P{UAS-Mad.FLAG} (Oh and Irvine, 2011)
Tagged with	T:Zzzz\FLAG (Oh and Irvine, 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Mad[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] has no significant effect on eye size. (Oh and Irvine, 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C}, tkv^{Q253D.Scer\UAS.cNc} has increased cell number \| somatic clone phenotype, enhanceable by yki^{S111A.S250A.Scer\UAS.T:SV5\V5}, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011)
Enhancer of
Statement Reference Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF is an enhancer of size defective \| adult stage phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF is an enhancer of visible \| adult stage phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} is an enhancer of increased cell number \| somatic clone phenotype of Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} is an enhancer of increased cell number \| somatic clone phenotype of Scer\GAL4^{Scer\FRT.Act5C}, yki^{S111A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011)
Suppressor of
Statement Reference tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF is a suppressor of size defective \| adult stage phenotype of Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa (Oh and Irvine, 2011) tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF is a suppressor of visible \| adult stage phenotype of Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa (Oh and Irvine, 2011)
NOT Suppressor of
Statement Reference tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} is a non-suppressor of decreased cell number \| somatic clone phenotype of Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa (Oh and Irvine, 2011)
Other
Statement Reference Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C}, tkv^{Q253D.Scer\UAS.cNc} has increased cell number \| somatic clone phenotype (Oh and Irvine, 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C}, tkv^{Q253D.Scer\UAS.cNc} has wing disc \| somatic clone phenotype, enhanceable by yki^{S111A.S250A.Scer\UAS.T:SV5\V5}, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011)
Enhancer of
Statement Reference Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, yki^{S111A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} is an enhancer of wing disc \| somatic clone phenotype of Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} is an enhancer of wing disc \| somatic clone phenotype of Scer\GAL4^{Scer\FRT.Act5C}, yki^{S111A.S250A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011)
Suppressor of
Statement Reference tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa (Oh and Irvine, 2011)
NOT Suppressor of
Statement Reference tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} is a non-suppressor of wing disc \| somatic clone phenotype of Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa (Oh and Irvine, 2011)
Other
Statement Reference Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C}, tkv^{Q253D.Scer\UAS.cNc} has wing disc \| somatic clone phenotype (Oh and Irvine, 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of Mad[Scer\UAS.T:Zzzz\FLAG] enhances the eye overgrowth seen when yki[S111A.S250A.Scer\UAS.T:SV5\V5] is expressed under the control of Scer\GAL4[GMR.PF]. Co-expression of Mad[Scer\UAS.T:Zzzz\FLAG] and tkv[Q253D.Scer\UAS.cNc] in wing disc clones under the control of Scer\GAL4[Scer\FRT.Act5C] modestly enhances growth in lateral regions. Expression of yki[S111A.S250A.Scer\UAS.T:SV5\V5] enhances the overgrowth seen in wing disc clones expressing Mad[Scer\UAS.T:Zzzz\FLAG] and tkv[Q253D.Scer\UAS.cNc] under the control of Scer\GAL4[Scer\FRT.Act5C]. The phenotype seen in stronger than is seen when yki[S111A.S250A.Scer\UAS.T:SV5\V5] is expressed alone. Co-expression of tkv[Q253D.Scer\UAS.cNc] and Mad[Scer\UAS.T:Zzzz\FLAG] does not suppress the growth and survival defects seen when brk[Scer\UAS.cJa] is expressed in wing disc clones under the control of Scer\GAL4[Scer\FRT.Act5C]. Expression of yki[S168A.Scer\UAS.T:SV5\V5] partially suppresses the underproliferation seen when brk[Scer\UAS.cJa] is expressed in wing disc clones under the control of Scer\GAL4[Scer\FRT.Act5C]. Co-expression of tkv[Q253D.Scer\UAS.cNc] suppresses the growth defects further, producing some clone overgrowth, whilst the addition of Mad[Scer\UAS.T:Zzzz\FLAG] increases clone overgrowth even further. Co-expression of Mad[Scer\UAS.T:Zzzz\FLAG] and tkv[Q253D.Scer\UAS.cNc] suppresses the small eye phenotype seen when brk[Scer\UAS.cJa] is expressed under the control of Scer\GAL4[GMR.PF]. (Oh and Irvine, 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Mad^{Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Oh and Irvine, 2011, Dev. Cell 20(1): 109--122 Cooperative Regulation of Growth by Yorkie and Mad through bantam. [FBrf0212763]

Allele Dmel\MadScer\UAS.T:Zzzz\FLAG

Allele Dmel\Mad^{Scer\UAS.T:Zzzz\FLAG}