FlyBase Allele Report: Dmel\Elp3[Δ4]

General Information

Symbol

Dmel\Elp3^Δ4

Species

D. melanogaster

Name

FlyBase ID

FBal0269085

Feature type

allele

Associated gene

Dmel\Elp3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Miśkiewicz et al., 2011)

Progenitor genotype

P{SUPor-P}Elp3^KG02386

(Miśkiewicz et al., 2011)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of Elp3.

(Miśkiewicz et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage (with Elp3^Δ3)

(Miśkiewicz et al., 2011)

lethal (with Elp3¹)

(Miśkiewicz et al., 2011)

lethal (with Elp3²)

(Miśkiewicz et al., 2011)

lethal (with Elp3^Δ3)

(Miśkiewicz et al., 2011)

Phenotype Manifest In

cytoskeleton of presynaptic active zone (with Elp3^Δ3)

(Miśkiewicz et al., 2011)

external sensory organ precursor cell | somatic clone

(Planelles-Herrero et al., 2022)

Detailed Description

Statement

Reference

T-bars at the neuromuscular junction in third instar Elp3^Δ3/Elp3^Δ4 larvae have large protrusions that extend into the cytoplasm. The T-bar platforms of the mutant larvae are increased in size compared to wild type, and the number of synaptic vesicles in direct contact with the T-bar is increased.

The average excitatory junctional current (EJC) amplitude at the neuromuscular junction is increased compared to wild type in Elp3^Δ3/Elp3^Δ4 third instar larvae. The amplitude of the spontaneous mEJC is also increased in the mutant larvae, while mEJC frequency is not significantly different from wild type.

(Miśkiewicz et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Elp3^Δ3/Elp3^Δ4 has lethal phenotype, suppressible by Scer\GAL4^Act.PU/Hsap\ELP3^UAS.cMa

(Miśkiewicz et al., 2011)

Elp3^Δ3/Elp3^Δ4 has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Hsap\ELP3^UAS.cMa/Scer\GAL4^C57

(Miśkiewicz et al., 2011)

NOT suppressed by

Statement

Reference

Elp3^Δ3/Elp3^Δ4 has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by Scer\GAL4^nSyb.PS/Hsap\ELP3^UAS.cMa

(Miśkiewicz et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ELP3^Scer\UAS.cMa under the control of Scer\GAL4^C57, but not under the control of Scer\GAL4^n-syb.PS, rescues the increased spontaneous excitatory junctional current amplitude which is seen at the neuromuscular junction in Elp3^Δ3/Elp3^Δ4 third instar larvae.

(Miśkiewicz et al., 2011)

Complementation and Rescue Data

Complements

morgue^EP1184

(Miśkiewicz et al., 2011)

Fails to complement

Elp3²

(Miśkiewicz et al., 2011)

Elp3¹

(Miśkiewicz et al., 2011)

Rescued by

Elp3^Δ3/Elp3^Δ4 is rescued by Elp3^C.GFP