Allele Avic\GFP^{Cameleon2.0.Scer\UAS}

General Information
Symbol	Avic\GFP^{Cameleon2.0.Scer\UAS}	Species	A. victoria
Name		FlyBase ID	FBal0269232
Feature type	allele	Associated gene	Avic\GFP

Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the "Yellow Cameleon 2.0" Ca[2+] sensor. This protein consists of two different chromophores (ECFP and EYFP variants of Avic\GFP respectively) separated by a calcium binding region (T:Xlae\Cam). Ca[2+]-binding to T:Xlae\Cam results in a conformational change that can be detected as a change in the efficacy of fluorescence resonance energy transfer (FRET) between the two Avic\GFP variants. (Reiff et al., 2005)
Carried in construct	P{UAS-Cameleon.2.0} (Mank et al., 2006, Reiff et al., 2002, Reiff et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Avic\GFP[Cameleon2.0.Scer\UAS], driven by Scer\GAL4[elav-PG159], does not affect the physiological and morphological development of neuromuscular junctions. (Reiff et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Scer\GAL4[elav-C155]-mediated expression of Avic\GFP[Cameleon2.0.Scer\UAS] does not affect the neurological phenotype of GluRIIA[AD9] mutants. Scer\GAL4[elav-PG159]-mediated expression of Avic\GFP[Cameleon2.0.Scer\UAS] does not affect the phenotype of pAbp[k10109]/+ mutant larvae: these mutants still have larger evoked postsynaptic currents and significantly more boutons compared to wild type. (Reiff et al., 2002)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Avic\GFP^{Cameleon2.0.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Mank et al., 2006, Biophys. J. 90(5): 1790--1796 A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change. [FBrf0189879] Reiff et al., 2005, J. Neurosci. 25(19): 4766--4778 In vivo performance of genetically encoded indicators of neural activity in flies. [FBrf0187903] Reiff et al., 2002, J. Neurosci. 22(21): 9399--9409 Differential regulation of active zone density during long-term strengthening of Drosophila neuromuscular junctions. [FBrf0152148]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Allele Avic\GFPCameleon2.0.Scer\UAS

Allele Avic\GFP^{Cameleon2.0.Scer\UAS}