Allele Hsap\SNCA^{A53T.Scer\UAS.cKa}

General Information
Symbol	Hsap\SNCA^{A53T.Scer\UAS.cKa}	Species	H. sapiens
Name		FlyBase ID	FBal0269386
Feature type	allele	Associated gene	Hsap\SNCA

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Karpinar et al., 2009) in vitro construct - regulatory fusion (Karpinar et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a mutant form of Hsap\SNCA (amino acid replacement A53T). (Karpinar et al., 2009)
Carried in construct	M{UAS-αSyn.A53T} (Karpinar et al., 2009, Butler et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	gravitaxis defective \| adult stage, with Scer\GAL4^elav.PU (Karpinar et al., 2009) gravitaxis defective \| adult stage \| conditional, with Scer\GAL4^Ddc.PL (Butler et al., 2012) neuroanatomy defective \| adult stage, with Scer\GAL4^Ddc.PL (Butler et al., 2012) neuroanatomy defective \| adult stage \| conditional, with Scer\GAL4^elav.PU (Karpinar et al., 2009) short lived, with Scer\GAL4^Ddc.PL (Butler et al., 2012)
Phenotype Manifest In
	dopaminergic neuron \| adult stage, with Scer\GAL4^Ddc.PL (Butler et al., 2012) dopaminergic neuron \| adult stage \| adult stage \| conditional, with Scer\GAL4^elav.PU (Karpinar et al., 2009)
Detailed Description
	Statement Reference Expression of two copies of Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[Ddc.PL] results in a significant decrease in longevity (flies expressing one copy of Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[Ddc.PL] have normal longevity). The number of ple-positive neurons in the PPM1/2 cluster is reduced compared to wild type in adults expressing Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[Ddc.PL]. Adults expressing Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[Ddc.PL] show an age-related deficit in climbing ability. (Butler et al., 2012) The number of dopaminergic neurons in the dorsolateral (DL) and dorsomedial (DM) clusters in the brain of flies expressing Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[elav.PU] is normal in 2 day old adults, but is reduced compared to age-matched controls in 29 day old adults. 25-30 day old flies expressing Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[elav.PU] have reduced climbing ability compared to age-matched controls. (Karpinar et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\SNCA^{A53T.Scer\UAS.cKa}, Scer\GAL4^Ddc.PL has gravitaxis defective \| adult stage \| conditional phenotype, suppressible \| partially by Hsap\TRAP1^Scer\UAS.cBa, Scer\GAL4^Ddc.PL (Butler et al., 2012) Hsap\SNCA^{A53T.Scer\UAS.cKa}, Scer\GAL4^Ddc.PL has neuroanatomy defective \| adult stage phenotype, suppressible by Hsap\TRAP1^Scer\UAS.cBa, Scer\GAL4^Ddc.PL (Butler et al., 2012)
NOT suppressed by
Statement Reference Hsap\SNCA^{A53T.Scer\UAS.cKa}, Scer\GAL4^Ddc.PL has short lived phenotype, non-suppressible by Hsap\TRAP1^Scer\UAS.cBa, Scer\GAL4^Ddc.PL (Butler et al., 2012)
Phenotype Manifest In
Suppressed by
Statement Reference Hsap\SNCA^{A53T.Scer\UAS.cKa}, Scer\GAL4^Ddc.PL has dopaminergic neuron \| adult stage phenotype, suppressible by Hsap\TRAP1^Scer\UAS.cBa, Scer\GAL4^Ddc.PL (Butler et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\TRAP1[Scer\UAS.cBa] suppresses the reduction in the number of ple-positive neurons in the PPM1/2 cluster which is seen in adults expressing Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[Ddc.PL]. Co-expression of Hsap\TRAP1[Scer\UAS.cBa] significantly rescue the age-related deficit in climbing ability seen in adults expressing Hsap\SNCA[A53T.Scer\UAS.cKa] under the control of Scer\GAL4[Ddc.PL]. (Butler et al., 2012)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\SNCA^{A53T.Scer\UAS.cKa}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Butler et al., 2012, PLoS Genet. 8(2): e1002488 The Mitochondrial Chaperone Protein TRAP1 Mitigates α-Synuclein Toxicity. [FBrf0217423] Karpinar et al., 2009, EMBO J. 28(20): 3256--3268 Pre-fibrillar alpha-synuclein variants with impaired beta-structure increase neurotoxicity in Parkinson's disease models. [FBrf0208949]

Allele Hsap\SNCAA53T.Scer\UAS.cKa

Allele Hsap\SNCA^{A53T.Scer\UAS.cKa}