FB2025_05 , released December 11, 2025
Allele: Dmel\ScrSynth.E19G.UAS.N.N-Venus
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General Information
Symbol
Dmel\ScrSynth.E19G.UAS.N.N-Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0269441
Feature type
allele
Associated gene
Dmel\Scr
Associated Insertion(s)
Carried in Construct
P{UAS-VN-Scr.Synth.E19G}
Key Links
Transgenic product class
inframe_deletion
(Papadopoulos et al., 2012)
missense_variant
(Papadopoulos et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Papadopoulos et al., 2012)
missense_variant
(Papadopoulos et al., 2012)
Mutagen
in vitro construct
(Papadopoulos et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-VN-Scr.Synth.E19G}
Cytology
Description

UASt regulatory sequences drive expression of a synthetic version of Scr consisting of the YPWM motif, the homeodomain and the small C-terminus. The protein contains the mutation E19G. The protein is tagged at the N-terminal end with N-Venus, a non-fluorescent split fluorescent protein fragment. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.

(Papadopoulos et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Scr
Tagged with
N-Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Co-expression of ScrSynth.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4hs.PB does not result in the formation of ectopic salivary glands in the embryo.

      Co-expression of ScrSynth.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4nullo.PG does not result in the transformation of mesothoracic and metathoracic segments towards prothoracic segments in the embryo.

      (Papadopoulos et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Construct for BiFC analysis (reconstitution of YFP fluorescence occurs when the T:Avic\GFPVN tag in this construct is brought together in the cell with a protein containing the T:Avic\GFPVC tag).

      (Papadopoulos et al., 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ScrSynth.E19G.Scer\UAS.N.T:Avic\GFP-VN
      ScrSynth.E19G.UAS.N.N-Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (1)