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General Information
Symbol
Dmel\Dop1R1UAS.Tango
Species
D. melanogaster
Name
FlyBase ID
FBal0269826
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of the Dop1R1 receptor fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the Dop1R1UAS.Tango allele). The downstream coding region consists of Arr1 fused to the TEV\NIa protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Dop1R1 receptor by its ligand, the Arr1::TEV\NIa protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

The Tango system (first described in Barnea et al., 2008 - PMID:18165312 ) is used to report endogenous neuromodulator release in vivo with anatomic specificity. The presence of a 2A peptide sequence allows dicistronic expression of two coding regions within the same construct by forcing the ribosome to skip from a glycine to a proline without forming a peptide bond, resulting in stochiometric production of two proteins. These proteins are: 1) a neuromodulator receptor tethered to T:Ivir\HA1 tagged Ecol\lexA via a TEV protease cleavage site (T:Zzzz\TEV.CS) and 2) a Arr1-Zzzz\NIa fusion protein. On binding of the neuromodulator to the Ecol\lexA-T:Ivir\HA1 bound receptor, the Arr1-Zzzz\NIa fusion protein cleaves Ecol\lexA T:Ivir\HA1 from the receptor, allowing it to translocate to the nucleus where it can drive expression of a Ecol\lexAop-driven reporter.

The P{UAS-DopR-Tango} construct can be used as a reporter of dopamine release in vivo.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Dop1R1Scer\UAS.Tango.T:Ecol\lexA
Dop1R1UAS.Tango
DopRScer\UAS.Tango.T:Ecol\lexA
Name Synonyms
Secondary FlyBase IDs
    References (2)