|Feature type||allele||Associated gene||Dmel\pburs|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
Depending on the exact genotype, 30-50% of homozygous and hemizygous pburs[exc] mutant pupae complete metamorphosis and eclose. 100% of these adults fail to inflate their wings and their exoskeleton does not harden and shows abnormal melanization. The remainder of the pharate adults express a spectrum of morphological defects diagnostic of failures at pupal ecdysis, such as partial head eversion, abnormally short legs and wings, and the presence of a persistent space between the end of the abdomen and the overlying puparium. pburs[exc] homo- and hemizygous mutants do not display a difference in the duration of pre-ecdysis compared to controls. They do however, express a longer and more variable period between pullback and head eversion. Indeed, whereas 90-100% of wild-type animals evert their heads within 90 seconds of pullback, the majority of pburs[exc] hemizygotes take >90 seconds to do so, with 20-40% failing to do so. Over 50% of pburs[exc] mutant adults exhibit a reduced head, with 20-40% exhibiting a severely reduced or absent head, compared to wild-type. These pharate adults also show significantly shorter legs and wings. Transheterozygous pburs[pupal1]/pburs[exc] mutants show partially expanded or incomplete wings, leg extension defects and abnormal tanning.
|Phenotype Manifest In|
pburs[exc]; Ccap[exc] double mutants exhibit normal viability during the larval stages, but express severe defects in pupation. Although they express pre-ecdysis behavior, this phase rarely ends with a distinct pullback of the front end of the animal from the puparium. Instead, pullback is typically weak or absent, and pre-ecdysis transitions into a weaker pre-ecdysis-like behavior, which later resembles post-ecdysis behaviors. The vast majority of animals fail to express any head eversion behaviors within 30 minutes and the morphology of the resulting pharate adults is also severely affected, with 70-90% showing no head. The presence of Ccap[t.1755] and pburs[+t.1886] transgenes rescues these defects.
|Complementation & Rescue Data|
A single copy of pburs[+t.1886] rescues the delay between pullback and head eversion during ecdysis, along with the reduced or absent head, and shorter legs and wings found in pburs[exc] mutants. The presence of pburs[+t.1886] rescues the defects found in pburs[pupal1]/pburs[exc] transheterozygotes.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 1 )|