Allele Dmel\pburs^exc

General Information
Symbol	Dmel\pburs^exc	Species	D. melanogaster
Name		FlyBase ID	FBal0269832
Feature type	allele	Associated gene	Dmel\pburs

Allele class
Mutagen	FLPase
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	FLPase (Lahr et al., 2012)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference FLPase-mediated excision of PBac{RB}e02061 (4.2kb 5' of pburs) and P{XP}d02171 (7.2kb 3' of pburs) removes the pburs coding region. (Lahr et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	adult head (Lahr et al., 2012) adult head (with pburs^pupal1) (Lahr et al., 2012) leg (Lahr et al., 2012) leg (with pburs^pupal1) (Lahr et al., 2012) pharate adult (Lahr et al., 2012) pharate adult (with pburs^pupal1) (Lahr et al., 2012) pupal cuticle (Lahr et al., 2012) pupal cuticle (with pburs^pupal1) (Lahr et al., 2012) wing (Lahr et al., 2012) wing (with pburs^pupal1) (Lahr et al., 2012)
Detailed Description
	Statement Reference Depending on the exact genotype, 30-50% of homozygous and hemizygous pburs[exc] mutant pupae complete metamorphosis and eclose. 100% of these adults fail to inflate their wings and their exoskeleton does not harden and shows abnormal melanization. The remainder of the pharate adults express a spectrum of morphological defects diagnostic of failures at pupal ecdysis, such as partial head eversion, abnormally short legs and wings, and the presence of a persistent space between the end of the abdomen and the overlying puparium. pburs[exc] homo- and hemizygous mutants do not display a difference in the duration of pre-ecdysis compared to controls. They do however, express a longer and more variable period between pullback and head eversion. Indeed, whereas 90-100% of wild-type animals evert their heads within 90 seconds of pullback, the majority of pburs[exc] hemizygotes take >90 seconds to do so, with 20-40% failing to do so. Over 50% of pburs[exc] mutant adults exhibit a reduced head, with 20-40% exhibiting a severely reduced or absent head, compared to wild-type. These pharate adults also show significantly shorter legs and wings. Transheterozygous pburs[pupal1]/pburs[exc] mutants show partially expanded or incomplete wings, leg extension defects and abnormal tanning. (Lahr et al., 2012)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference pburs[exc]; Ccap[exc] double mutants exhibit normal viability during the larval stages, but express severe defects in pupation. Although they express pre-ecdysis behavior, this phase rarely ends with a distinct pullback of the front end of the animal from the puparium. Instead, pullback is typically weak or absent, and pre-ecdysis transitions into a weaker pre-ecdysis-like behavior, which later resembles post-ecdysis behaviors. The vast majority of animals fail to express any head eversion behaviors within 30 minutes and the morphology of the resulting pharate adults is also severely affected, with 70-90% showing no head. The presence of Ccap[t.1755] and pburs[+t.1886] transgenes rescues these defects. (Lahr et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	pburs^exc/pburs^pupal1 is rescued by pburs^+t.1886 (Lahr et al., 2012) pburs^exc is rescued by pburs^+t.1886 (Lahr et al., 2012)
Comments	A single copy of pburs[+t.1886] rescues the delay between pullback and head eversion during ecdysis, along with the reduced or absent head, and shorter legs and wings found in pburs[exc] mutants. The presence of pburs[+t.1886] rescues the defects found in pburs[pupal1]/pburs[exc] transheterozygotes. (Lahr et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pburs^exc (Lahr et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Lahr et al., 2012, J. Neurosci. 32(20): 6819--6829 Genetic analysis of ecdysis behavior in Drosophila reveals partially overlapping functions of two unrelated neuropeptides. [FBrf0218345]

Allele Dmel\pbursexc

Allele Dmel\pburs^exc