Allele Dmel\foxo^Δ94

General Information
Symbol	Dmel\foxo^Δ94	Species	D. melanogaster
Name		FlyBase ID	FBal0269838
Feature type	allele	Associated gene	Dmel\foxo

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Slack et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	foxo^BG01018 (Slack et al., 2011)
Nature of the lesion	Statement Reference Imprecise excision of P{GT1}foxo[BG01018] generate a deletion that spans over 20kb of the foxo locus, removing part of the predicted promoter region as well as several coding exons. (Slack et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective (Slack et al., 2011) developmental rate defective (with foxo²⁵) (Slack et al., 2011) long lived \| nutrition conditional (Slack et al., 2011) short lived (Slack et al., 2011) small body (Slack et al., 2011) small body (with foxo²⁵) (Slack et al., 2011) viable (Slack et al., 2011)
Phenotype Manifest In
	embryonic/larval neuromuscular junction (Nechipurenko and Broihier, 2012) microtubule (Nechipurenko and Broihier, 2012) wing (Slack et al., 2011) wing (with foxo²⁵) (Slack et al., 2011)
Detailed Description
	Statement Reference foxo[Δ94] mutant neuromuscular junctions display enhanced microtubule stability compared to controls. (Nechipurenko and Broihier, 2012) foxo[Δ94] homozygotes and foxo[Δ94]/foxo[25] transheterozygotes are delayed in egg-adult development time and are smaller in size compared to controls, with a significant reduction in both body weight and wing area. Homozygous foxo[Δ94] females are shorter lived than controls and lay fewer eggs. foxo[Δ94] mutant clones appear normal in size, suggesting that foxo does not act cell-autonomously to restrict cell proliferation or growth. foxo[Δ94] females display an increase in lifespan under dietary restriction. (Slack et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference chico¹, foxo^Δ94 has lethal phenotype, suppressible by foxo^{Scer\UAS.P\T.cSa}/Scer\GAL4^da.G32 (Slack et al., 2011)
NOT suppressed by
Statement Reference foxo^Δ94 has viable \| RU486 conditional phenotype, non-suppressible by InR^{K1409A.Scer\UAS}/Scer\GAL4^da.Switch.PT (Slack et al., 2011) foxo^Δ94 has viable \| RU486 conditional phenotype, non-suppressible by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^da.Switch.PT (Slack et al., 2011) foxo^Δ94 has viable phenotype, non-suppressible by InR^{K1409A.Scer\UAS}/Scer\GAL4^da.G32 (Slack et al., 2011) foxo^Δ94 has viable phenotype, non-suppressible by rpr^Scer\UAS.C/Scer\GAL4^Ilp3.PB (Slack et al., 2011)
Suppressor of
Statement Reference foxo^Δ94 is a suppressor \| partially of long lived \| drug conditional phenotype of Scer\GAL4^Ilp3.PB, rpr^Scer\UAS.C (Slack et al., 2011) foxo^Δ94 is a suppressor \| partially of long lived phenotype of InR^{K1409A.Scer\UAS}, Scer\GAL4^da.Switch.PT (Slack et al., 2011) foxo^Δ94 is a suppressor of developmental rate defective phenotype of InR^{K1409A.Scer\UAS}, Scer\GAL4^da.G32 (Slack et al., 2011) foxo^Δ94 is a suppressor of long lived \| RU486 conditional phenotype of InR^{K1409A.Scer\UAS}, Scer\GAL4^da.Switch.PT (Slack et al., 2011) foxo^Δ94 is a suppressor of long lived \| RU486 conditional phenotype of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^da.Switch.PT (Slack et al., 2011) foxo^Δ94 is a suppressor of long lived phenotype of Scer\GAL4^Ilp3.PB, rpr^Scer\UAS.C (Slack et al., 2011)
NOT Suppressor of
Statement Reference foxo^Δ94 is a non-suppressor of small body phenotype of Scer\GAL4^Ilp3.PB, rpr^Scer\UAS.C (Slack et al., 2011) foxo^Δ94 is a non-suppressor of viable \| RU486 conditional phenotype of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^da.Switch.PT (Slack et al., 2011)
Other
Statement Reference chico¹, foxo^Δ94 has lethal phenotype (Slack et al., 2011) Ilp2¹, Ilp3¹, Ilp5¹, foxo^Δ94 has lethal phenotype (Slack et al., 2011) Lnk^Del29, foxo^Δ94 has lethal phenotype (Slack et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference foxo^Δ94 is a suppressor of eye phenotype of InR^{K1409A.Scer\UAS}, Scer\GAL4^ey.PH (Slack et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of foxo[Scer\UAS.P\T.cSa] within the median neurosecretary cells (MNCs) under the control of Scer\GAL4[da.G32] rescues the lethality seen in chico[1];foxo[Δ94] mutants. Expression of InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.G32] in foxo[Δ94] mutant flies results in an age-related increase in survival compared to controls, but this is less than in wild-type flies expressing InR[K1409A.Scer\UAS]. A foxo[Δ94] mutant background suppresses the extension in lifespan seen upon RU486-induced expression of InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.Switch.PT]. Expression of InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.G32] does not affect the viability of foxo[Δ94] flies. Late ablation of the mNSCs through expression of rpr[Scer\UAS.C] under the control of Scer\GAL4[Ilp3.PB] does not affect the viability of foxo[Δ94] flies. Adult onset ubiquitous expression of InR[K1409A.Scer\UAS] or Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of the RU486-inducible Scer\GAL4[da.Switch.PT] has no effect on the viability of foxo[Δ94] flies. foxo[Δ94] mutant females expressing InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.G32] show reduced egg laying compared with controls. foxo[Δ94] mutant females expressing InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.Switch.PT] treated with RU486 lay significantly fewer eggs than their uninduced controls. foxo[Δ94] mutant flies expressing InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.G32] all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background). A foxo[Δ94] background suppresses the DTT resistance seen in flies expressing InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.G32]. A foxo[Δ94] background fully suppresses the tissue-restricted growth inhibition seen in the eye upon expression of InR[K1409A.Scer\UAS] under the control of Scer\GAL4[ey.PH]. Removal of foxo through a foxo[Δ94] background does not suppress the developmental delay or reduced body size seen in flies expressing InR[K1409A.Scer\UAS] under the control of Scer\GAL4[da.G32]. A foxo[Δ94] mutant background suppresses the extension in lifespan seen upon RU486-induced expression of Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[da.Switch.PT]. Adult onset ubiquitous expression of Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of the RU486-inducible Scer\GAL4[da.Switch.PT] has no effect on the viability of foxo[Δ94] flies. A foxo[Δ94] mutant background does not affect the decrease in egg-laying found upon adult-specific ubiquitous expression of Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[da.Switch.PT]. foxo[Δ94] mutant flies expressing Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[da.Switch.PT] all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background). A foxo[Δ94] background suppresses the DTT resistance seen in flies expressing Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[da.Switch.PT]. foxo[Δ94] mutant flies expressing Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[da.G32] all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background). A foxo[Δ94] background suppresses the lifespan of female flies expressing rpr[Scer\UAS.C] under the control of Scer\GAL4[Ilp3.PB], almost to wild-type levels. foxo[Δ94] mutant females expressing rpr[Scer\UAS.C] under the control of Scer\GAL4[da.G32] show reduced egg laying compared with controls. In a foxo[Δ94] mutant background, Scer\GAL4[Ilp3.PB] driven expression of rpr[Scer\UAS.C] leads to significantly fewer eggs being laid than in controls. (Slack et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	42220 w¹¹¹⁸; foxo^Δ94/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	dfoxo^Δ94 (Slack et al., 2011) foxo^Δ94 (Rynes et al., 2012, Nechipurenko and Broihier, 2012, )
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Rynes et al., 2012, Mol. Cell. Biol. 32(19): 3949--3962 Activating transcription factor 3 regulates immune and metabolic homeostasis. [FBrf0219385] Slack et al., 2011, Aging Cell 10(5): 735--748 dFOXO-independent effects of reduced insulin-like signaling in Drosophila. [FBrf0215229]

Allele Dmel\foxoΔ94

Allele Dmel\foxo^Δ94