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General Information
Symbol
Dmel\foxoΔ94
Species
D. melanogaster
Name
FlyBase ID
FBal0269838
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dfoxoΔ94, dFOXO94
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{GT1}foxoBG01018 generate a deletion that spans over 20kb of the foxo locus, removing part of the predicted promoter region as well as several coding exons.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

foxoΔ94 homozygous adults show a significant decrease in lifespan, a significant decrease in locomotor capacity (in a startle-induced climbing assay), a significant and progressive decrease in the number of dopaminergic PAM neurons, but not of PPM1, PPM2, PPM3, PPL1, PPL2a/b, PPL2c or PAL neurons, and a significant and progressive decrease in the number of autophagosomes (i.e. Atg-8-positive puncta) in the PAM neuron region, but not in the PAL neuron region, in the adult brain, as compared to heterozygous and wild-type controls.

foxoΔ94 mutant flies show a similar reduction in lifespan when fed excess sugar as wild type. foxoΔ94 mutation causes a slight but significant increase in triacylglycerol (TAG) levels when flies are fed 8x sugar food.

foxoΔ94 suppresses the extension in lifespan seen in flies fed excess sugar when Scer\GAL4Ilp3.PB-expressing median neurosecretory cells (mNSCs) have been ablated.

foxoΔ94 homozygous females exhibit short lifespans.

foxoΔ94/Df(3R)Exel8159 mutant starved larvae that express foxoNK.Scer\UAS under the control of Scer\GAL4phm.PO reach the 'critical weight' ecdysone peak later and at larger sizes than controls, but earlier and at smaller sizes than larvae that express foxoScer\UAS.cKc.

When foxoScer\UAS.cKc is expressed under the control of Scer\GAL4phm.PO in foxoΔ94/Df(3R)Exel8159 background most larvae do not survive to the third instar larval stage.

Expression of foxoScer\UAS.cKc in a foxoΔ94/Df(3R)Exel8159 mutant background in the oenocytes under the control of Scer\GAL4Desat1.PB, or in the corpora allata under the control of Scer\GAL4Aug21 does not affect the duration of the third instar larval stage (L3) or final body size in starved larvae. Expression in the ring gland, oenocytes and the corpora allata under the control of Scer\GAL4P0206 delays metamorphosis and increases body size at the 'critical weight' ecdysone peak compared to parental controls. Feeding ecdysone to Scer\GAL4P0206>foxoScer\UAS.cKc foxoΔ94/Df(3R)Exel8159 starved early third instar larvae eliminates this delay in metamorphosis.

Homozygous foxoΔ94 mutants do not exhibit any activity or sleep defects.

Unlike in controls, treating foxoΔ94 mutant flies with tolbutamide does not increase daytime activity.

foxoΔ94 mutants do not exhibit defects in locomotory ability.

foxoΔ94 mutant neuromuscular junctions display enhanced microtubule stability compared to controls.

foxoΔ94 homozygotes and foxoΔ94/foxo25 transheterozygotes are delayed in egg-adult development time and are smaller in size compared to controls, with a significant reduction in both body weight and wing area. Homozygous foxoΔ94 females are shorter lived than controls and lay fewer eggs.

foxoΔ94 mutant clones appear normal in size, suggesting that foxo does not act cell-autonomously to restrict cell proliferation or growth.

foxoΔ94 females display an increase in lifespan under dietary restriction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference

foxoΔ94 has short lived phenotype, non-suppressible by chico1/chico[+]

foxoΔ94 has viable phenotype, non-suppressible by InRK1409A.UAS/Scer\GAL4da.G32

foxoΔ94 has viable | RU486 conditional phenotype, non-suppressible by Pi3K92ED954A.UAS.Tag:MYC/Scer\GAL4da.Switch.PT

foxoΔ94 has viable phenotype, non-suppressible by rprUAS.C/Scer\GAL4Ilp3.PB

foxoΔ94 has viable | RU486 conditional phenotype, non-suppressible by InRK1409A.UAS/Scer\GAL4da.Switch.PT

Suppressor of
Statement
Reference

foxo[+]/foxoΔ94 is a suppressor of visible phenotype of Hsap\APLP1UAS.cMa, Scer\GAL4sd-SG29.1

foxo[+]/foxoΔ94 is a suppressor of visible phenotype of Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237

foxoΔ94 is a suppressor of long lived | RU486 conditional phenotype of Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4da.Switch.PT

foxoΔ94 is a suppressor | partially of long lived | drug conditional phenotype of Scer\GAL4Ilp3.PB, rprUAS.C

foxoΔ94 is a suppressor of long lived | RU486 conditional phenotype of InRK1409A.UAS, Scer\GAL4da.Switch.PT

NOT Suppressor of
Statement
Reference

foxoΔ94 is a non-suppressor of decreased fecundity | female | RU486 conditional phenotype of Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4da.Switch.PT

foxoΔ94 is a non-suppressor of viable | RU486 conditional phenotype of Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4da.Switch.PT

foxoΔ94 is a non-suppressor of small body phenotype of Scer\GAL4Ilp3.PB, rprUAS.C

Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The progressive decrease in the number of adult PAM neurons observed in foxoΔ94 homozygotes is enhanced by the Scer\GAL4Fer2.2359-driven expression of Fer2miRNA.UAS.5 and is suppressed by the Scer\GAL4Fer2.2359-driven or Scer\GAL4GMR58E02-driven expression of Fer2UAS.Tag:FLAG; the expression of Fer2miRNA.UAS.5 under the control of Scer\GAL4Fer2.2359, however, does not affect the number of adult PAL cluster neurons observed in foxoΔ94 homozygotes. The decreased adult locomotion capacity and the decreased lifespan presented by foxoΔ94 homozygotes are partially suppressed by the expression of Fer2UAS.Tag:FLAG under the control of Scer\GAL4GMR58E02.

Expressing foxoNK.Scer\UAS and uspScer\UAS.cUa under the control of Scer\GAL4phm.PO in foxoΔ94/Df(3R)Exel8159 mutant larvae does not alter the age and size of the 'critical weight' ecdysone peak, nor does it alter final body size when compared to overexpressing foxoNK.Scer\UAS alone.

One copy of foxoΔ94 partially rescues the sleep defects seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32, specifically affecting day, but not night time phenotypes. Mutants show strongly reduced day activity and increased day sleep duration without changes in wakefulness (average activity per awake minute), and the number of day sleep bouts does not differ significantly from controls.

foxoΔ94/+ partially rescues the starvation stress phenotype of ArkCD4 flies.

A foxoΔ94 mutant background suppresses the Scer\GAL4ptc-559.1-->Hsap\APP695.Scer\UAS induced cell death in the larval wing disc and the loss of anterior cross vein found in these mutants.

A foxoΔ94 mutant background suppresses the induced scutellum phenotype found upon expression of Hsap\APP695.Scer\UAS under the control of Scer\GAL4ap-md544 and the Scer\GAL4sd-SG29.1-->Hsap\APP695.Scer\UAS-induced small wing phenotype.

A foxoΔ94 mutant background suppresses the Scer\GAL4ptc-559.1-->Hsap\APP695.Scer\UAS induced cell death in the larval wing disc and the loss of anterior cross vein found in these mutants.

Expression of foxoScer\UAS.P\T.cSa within the median neurosecretary cells (MNCs) under the control of Scer\GAL4da.G32 rescues the lethality seen in chico1;foxoΔ94 mutants.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 in foxoΔ94 mutant flies results in an age-related increase in survival compared to controls, but this is less than in wild-type flies expressing InRK1409A.Scer\UAS.

A foxoΔ94 mutant background suppresses the extension in lifespan seen upon RU486-induced expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.Switch.PT.

Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 does not affect the viability of foxoΔ94 flies.

Late ablation of the mNSCs through expression of rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB does not affect the viability of foxoΔ94 flies.

Adult onset ubiquitous expression of InRK1409A.Scer\UAS or Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of the RU486-inducible Scer\GAL4da.Switch.PT has no effect on the viability of foxoΔ94 flies.

foxoΔ94 mutant females expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 show reduced egg laying compared with controls.

foxoΔ94 mutant females expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.Switch.PT treated with RU486 lay significantly fewer eggs than their uninduced controls.

foxoΔ94 mutant flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32 all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).

A foxoΔ94 background suppresses the DTT resistance seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32.

A foxoΔ94 background fully suppresses the tissue-restricted growth inhibition seen in the eye upon expression of InRK1409A.Scer\UAS under the control of Scer\GAL4ey.PH.

Removal of foxo through a foxoΔ94 background does not suppress the developmental delay or reduced body size seen in flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4da.G32.

A foxoΔ94 mutant background suppresses the extension in lifespan seen upon RU486-induced expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT.

Adult onset ubiquitous expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of the RU486-inducible Scer\GAL4da.Switch.PT has no effect on the viability of foxoΔ94 flies.

A foxoΔ94 mutant background does not affect the decrease in egg-laying found upon adult-specific ubiquitous expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT.

foxoΔ94 mutant flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).

A foxoΔ94 background suppresses the DTT resistance seen in flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT.

foxoΔ94 mutant flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).

A foxoΔ94 background suppresses the lifespan of female flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB, almost to wild-type levels.

foxoΔ94 mutant females expressing rprScer\UAS.C under the control of Scer\GAL4da.G32 show reduced egg laying compared with controls.

In a foxoΔ94 mutant background, Scer\GAL4Ilp3.PB driven expression of rprScer\UAS.C leads to significantly fewer eggs being laid than in controls.

Xenogenetic Interactions
Statement
Reference

The younger female preference defect of males expressing Hsap\APP695.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Gr33a-GAL4 is significantly rescued in the presence of foxoΔ94/+ mutation.

foxoΔ94/+ significantly partially suppresses the increased cell death induced by expression of Hsap\APLP1Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4ptc-559.1, suppresses the blistered wing phenotype in flies with expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4sd-SG29.1, and suppresses the small scutellum phenotype seen with expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4pnr-MD237.

Complementation and Rescue Data
Comments

The expression of foxoUAS.cFa under the control of Scer\GAL4GMR58E02 rescues the decreased number of dopaminergic PAM neurons in the adult brain but enhances the decreased lifespan presented by foxoΔ94 homozygotes.

The presence of RU486 from day 2 of adulthood extends the median lifespan of foxoΔ94 female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 10%.

Expressing foxoScer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4Ilp2.215-55 driver, extends the lifespan of female flies in foxoΔ94 mutants.

Induction of foxoScer\UAS.cFa expression in the gut and fat body, under the control of Scer\GAL4Switch1.106 enhances the climbing ability of female flies throughout their lifespan, observed as an increase in the proportion of high, or combined medium and high, climbers. This enhancement is seen in a foxoΔ94 background.

Induction of foxoScer\UAS.cFa expression in adulthood, under the control of Scer\GAL4Switch1.106 (and RU486) has no effect on fecundity in foxoΔ94 mutants, but has an effect on body weight and protein content.

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Mutant
Wild-type
Stocks (1)
Notes on Origin
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (14)
References (29)