Allele Dmel\PGRP-SC1a^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\PGRP-SC1a^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0269840
Feature type	allele	Associated gene	Dmel\PGRP-SC1a

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bischoff et al., 2006) in vitro construct - RNAi (Bischoff et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat of a 558bp fragment of PGRP-SC1a coding sequence. (Bischoff et al., 2006)
Carried in construct	P{UAS-PGRP-SC1a.dsRNA} (Bischoff et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective, with Scer\GAL4^da.G32 (Bischoff et al., 2006)
Phenotype Manifest In

Detailed Description
	Statement Reference Infection of flies expressing PGRP-SC1a[dsRNA.Scer\UAS] (under the control of Scer\GAL4[da.G32]) with gram-negative bacteria induces an over-activation of the IMD pathway. This phenotype is not observed in non-infected flies or after pricking with a clean needle. Flies expressing PGRP-SC1a[dsRNA.Scer\UAS] under the control of Scer\GAL4[da.G32] do not exhibit increased susceptibility to E.cloacae, E.coli or B.subtilis infection. Mortality is 3-4 times higher in Scer\GAL4[da.G32]>PGRP-SC1a[dsRNA.Scer\UAS] larvae fed with E.coli or E.carotovora carotovora than in controls. A small percentage of the larvae that pupariate and eclose as pharate adults present developmental defects such as wing notching. Wing discs from flies fed with bacteria exhibit higher levels of cell death than controls. (Bischoff et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference PGRP-SC1a^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 has immune response defective phenotype, suppressible by PGRP-LC^ΔE (Bischoff et al., 2006)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The increase in lethality seen in larvae expressing PGRP-SC1a[dsRNA.Scer\UAS] (under the control of Scer\GAL4[da.G32]) is completely suppressed in a PGRP-LC[ΔE] background. (Bischoff et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Due to shared sequence with PGRP-SC1b, PGRP-SC1a[dsRNA.Scer\UAS] expression knocks-down PGRP-SC1a and PGRP-SC1b. (Bischoff et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	PGRP-SC1a^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Bischoff et al., 2006, PLoS Pathog. 2(2): e14 Downregulation of the Drosophila immune response by peptidoglycan-recognition proteins SC1 and SC2. [FBrf0192715]

Allele Dmel\PGRP-SC1adsRNA.Scer\UAS

Allele Dmel\PGRP-SC1a^{dsRNA.Scer\UAS}