|Name||Saccharomyces cerevisiae UAS construct a of Navarro||FlyBase ID||FBal0269852|
|Feature type||allele||Associated gene||Hsap\FXN|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[da.G32] results in lethality before eclosion. Ubiquitous expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[Act.PU] results in lethality. Expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[how-24B] leads to early to late pupal lethality. Viable progeny is produced when Hsap\FXN[Scer\UAS.cNa] is expressed under the control of any one of Scer\GAL4[neur-GAL4-A101], Scer\GAL4[repo] or Scer\GAL4[Appl.G1a]. However, expression of Hsap\FXN[Scer\UAS.cNa] at 29[o]C under the control of Scer\GAL4[Appl.G1a] results in pre-adult lethality. Ubiquitous expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[unspecified] results in muscular and nervous system defects in embryos at stage 16. The junctions of lateral transversal muscles 1, 2 and 3 with ventral longitudinal muscle display abnormalities compared with controls. These defects are likely due to deficient muscle growth. A few embryos exhibit abnormalities in muscular development resulting in disrupted muscular system. When compared to controls, a strong disorganization of the sensory axons in the peripheral nervous system (PNS) is observed. No abnormalities are found in the central nervous system (CNS). Expression of Hsap\FXN[Scer\UAS.cNa] in neural tissues via any one of Scer\GAL4[Appl.G1a], Scer\GAL4[neur-GAL4-A101] or Scer\GAL4[repo] results in statistically significant decline in the mean and maximum lifespans under both normal and elevated atmospheric oxygen conditions. In addition, flies expressing Hsap\FXN[Scer\UAS.cNa] with any one of these drivers exhibit reduced climbing ability in an age dependent manner compared with wild-type. Although flies expressing Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[Appl.G1a] exhibit locomotor deficit and shortened lifespan, these flies do not display brain abnormalities compared to control age-matched individuals. Expression of Hsap\FXN[Scer\UAS.cNa] in glial cells under the control of Scer\GAL4[repo] induces strong age-related degeneration in the cortex of the brain and neuropil vacuolization characterised by the presence of droplet-like structures. Ultrastructural analysis reveals complete morphological disruption of glial cells and the concomitant formation of lipid droplets. Several region of the brain exhibit clear mitochondrial phenotypes, such as abnormal morphology and vacuolization.
Hsap\FXNScer\UAS.cNa, Scer\GAL4neur-GAL4-A101 has short lived phenotype, suppressible by CatT:Mito-oat
|NOT suppressed by|
|Phenotype Manifest In|
Constitutive expression of Cat[T:Mito-oat] significantly extends the lifespan of flies expressing Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[neur-GAL4-A101]. Constitutive expression of Cat[T:Mito-oat] fails to extend the lifespan of flies expressing Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[repo]. Constitutive expression of Cat[T:Mito-oat] ameliorates the climbing deficiency that is induced by expression of Hsap\FXN[Scer\UAS.cNa] in glial cells under the control of Scer\GAL4[repo].
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Navarro
|Secondary FlyBase IDs|
|References ( 1 )|