Allele Hsap\FXN^Scer\UAS.cNa

General Information
Symbol	Hsap\FXN^Scer\UAS.cNa	Species	H. sapiens
Name	Saccharomyces cerevisiae UAS construct a of Navarro	FlyBase ID	FBal0269852
Feature type	allele	Associated gene	Hsap\FXN

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Navarro et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\FXN. (Navarro et al., 2011)
Carried in construct	P{UAS-FXN} (Navarro et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Appl.G1a (Navarro et al., 2011) lethal, with Scer\GAL4^da.G32 (Navarro et al., 2011) lethal \| P-stage, with Scer\GAL4^Act.PU (Navarro et al., 2011) lethal \| P-stage, with Scer\GAL4^how-24B (Navarro et al., 2011) locomotor behavior defective, with Scer\GAL4^Appl.G1a (Navarro et al., 2011) locomotor behavior defective, with Scer\GAL4^{neur-GAL4-A101} (Navarro et al., 2011) locomotor behavior defective, with Scer\GAL4^repo (Navarro et al., 2011) neuroanatomy defective, with Scer\GAL4^repo (Navarro et al., 2011) oxidative stress response defective, with Scer\GAL4^Appl.G1a (Navarro et al., 2011) oxidative stress response defective, with Scer\GAL4^{neur-GAL4-A101} (Navarro et al., 2011) oxidative stress response defective, with Scer\GAL4^repo (Navarro et al., 2011) short lived, with Scer\GAL4^Appl.G1a (Navarro et al., 2011) short lived, with Scer\GAL4^{neur-GAL4-A101} (Navarro et al., 2011) short lived, with Scer\GAL4^repo (Navarro et al., 2011) size defective \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) viable, with Scer\GAL4^{neur-GAL4-A101} (Navarro et al., 2011) viable, with Scer\GAL4^repo (Navarro et al., 2011)
Phenotype Manifest In
	A1-7 lateral transverse muscle 1 \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) A1-7 lateral transverse muscle 2 \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) A1-7 lateral transverse muscle 3 \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) adult cortex associated glial cell, with Scer\GAL4^repo (Navarro et al., 2011) adult neuropil associated glial cell, with Scer\GAL4^repo (Navarro et al., 2011) axon \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) brain, with Scer\GAL4^repo (Navarro et al., 2011) cortex region, with Scer\GAL4^repo (Navarro et al., 2011) mitochondrion, with Scer\GAL4^repo (Navarro et al., 2011) peripheral nervous system \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) presumptive embryonic/larval muscle system \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011) ventral longitudinal muscle \| embryonic stage 16, with Scer\GAL4^unspecified (Navarro et al., 2011)
Detailed Description
	Statement Reference Expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[da.G32] results in lethality before eclosion. Ubiquitous expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[Act.PU] results in lethality. Expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[how-24B] leads to early to late pupal lethality. Viable progeny is produced when Hsap\FXN[Scer\UAS.cNa] is expressed under the control of any one of Scer\GAL4[neur-GAL4-A101], Scer\GAL4[repo] or Scer\GAL4[Appl.G1a]. However, expression of Hsap\FXN[Scer\UAS.cNa] at 29[o]C under the control of Scer\GAL4[Appl.G1a] results in pre-adult lethality. Ubiquitous expression of Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[unspecified] results in muscular and nervous system defects in embryos at stage 16. The junctions of lateral transversal muscles 1, 2 and 3 with ventral longitudinal muscle display abnormalities compared with controls. These defects are likely due to deficient muscle growth. A few embryos exhibit abnormalities in muscular development resulting in disrupted muscular system. When compared to controls, a strong disorganization of the sensory axons in the peripheral nervous system (PNS) is observed. No abnormalities are found in the central nervous system (CNS). Expression of Hsap\FXN[Scer\UAS.cNa] in neural tissues via any one of Scer\GAL4[Appl.G1a], Scer\GAL4[neur-GAL4-A101] or Scer\GAL4[repo] results in statistically significant decline in the mean and maximum lifespans under both normal and elevated atmospheric oxygen conditions. In addition, flies expressing Hsap\FXN[Scer\UAS.cNa] with any one of these drivers exhibit reduced climbing ability in an age dependent manner compared with wild-type. Although flies expressing Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[Appl.G1a] exhibit locomotor deficit and shortened lifespan, these flies do not display brain abnormalities compared to control age-matched individuals. Expression of Hsap\FXN[Scer\UAS.cNa] in glial cells under the control of Scer\GAL4[repo] induces strong age-related degeneration in the cortex of the brain and neuropil vacuolization characterised by the presence of droplet-like structures. Ultrastructural analysis reveals complete morphological disruption of glial cells and the concomitant formation of lipid droplets. Several region of the brain exhibit clear mitochondrial phenotypes, such as abnormal morphology and vacuolization. (Navarro et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\FXN^Scer\UAS.cNa, Scer\GAL4^{neur-GAL4-A101} has short lived phenotype, suppressible by Cat^T:Mito-oat (Navarro et al., 2011) Hsap\FXN^Scer\UAS.cNa, Scer\GAL4^repo has locomotor behavior defective phenotype, suppressible by Cat^T:Mito-oat (Navarro et al., 2011)
NOT suppressed by
Statement Reference Hsap\FXN^Scer\UAS.cNa, Scer\GAL4^repo has short lived phenotype, non-suppressible by Cat^T:Mito-oat (Navarro et al., 2011)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Constitutive expression of Cat[T:Mito-oat] significantly extends the lifespan of flies expressing Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[neur-GAL4-A101]. Constitutive expression of Cat[T:Mito-oat] fails to extend the lifespan of flies expressing Hsap\FXN[Scer\UAS.cNa] under the control of Scer\GAL4[repo]. Constitutive expression of Cat[T:Mito-oat] ameliorates the climbing deficiency that is induced by expression of Hsap\FXN[Scer\UAS.cNa] in glial cells under the control of Scer\GAL4[repo]. (Navarro et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Hsap\FXN^Scer\UAS.cNa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Navarro
Secondary FlyBase IDs

References ( 1 )
Research paper	Navarro et al., 2011, PLoS ONE 6(7): e21017 Overexpression of human and fly frataxins in Drosophila provokes deleterious effects at biochemical, physiological and developmental levels. [FBrf0214483]

Allele Hsap\FXNScer\UAS.cNa

Allele Hsap\FXN^Scer\UAS.cNa