FlyBase Allele Report: Dmel\Cad99C[248A]

General Information

Symbol

Dmel\Cad99C^248A

Species

D. melanogaster

Name

FlyBase ID

FBal0270003

Feature type

allele

Associated gene

Dmel\Cad99C

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Schlichting et al., 2006)

Mutagen

P-element activity

(Schlichting et al., 2006)

Progenitor genotype

P{EP}Cad99C^GE21034

(Schlichting et al., 2006)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of Cad99C, spanning from 17980bp 5' to 156bp 5' of the translation start codon.

(Schlichting et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:29,827,624..29,845,448 [+]

Comment:

Deletion resulting from the imprecise excision of P{EP}Cad99C^GE21034, which removes sequences from 17980bp 5' to 156bp 5' of the Cad99C translation start codon.

(Schlichting et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile

(Eren-Ghiani et al., 2015)

Phenotype Manifest In

seminal vesicle

(Eren-Ghiani et al., 2015)

spermatid

(Eren-Ghiani et al., 2015)

spermatid cyst

(Eren-Ghiani et al., 2015)

spermatozoon

(Eren-Ghiani et al., 2015)

vitelline membrane

(Schlichting et al., 2006)

Detailed Description

Statement

Reference

Cad99C^248A/Cad99C^248A males are sterile, though motile sperm are occasionally observed in seminal vesicles and germ cells develop normally until late stages of spermiogenesis. Starting from the individualization stage, disorganized spermatid cysts with long coiled spermatid nuclei are observed and the few mature sperm that enter seminal vesicles display abnormal nuclear morphology (part of the nucleus has a coiled structure and unusually long or hooked nuclei are observed).

(Eren-Ghiani et al., 2015)

Eggs laid by homozygous females spontaneously collapse after deposition and do not develop into larvae. More than 75% of the eggs are permeable to the dye Neutral Red after dechorionation, suggesting a vitelline membrane defect.

(Schlichting et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cad99C^248A has male sterile phenotype, suppressible | partially by Prtl99C^386.EGFP

(Eren-Ghiani et al., 2015)

Other

Statement

Reference

Cad99C^248A, Df(2L)FDD-0338164 has male sterile phenotype

(Eren-Ghiani et al., 2015)

Cad99C[+]/Cad99C^248A, Prtl99C^MI10224 has male semi-sterile phenotype

(Eren-Ghiani et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cad99C^248A has spermatid phenotype, enhanceable by Df(2L)FDD-0338164/Df(2L)FDD-0338164

(Eren-Ghiani et al., 2015)

Suppressed by

Statement

Reference

Cad99C^248A has seminal vesicle phenotype, suppressible | partially by Prtl99C^386.EGFP

(Eren-Ghiani et al., 2015)

Cad99C^248A has spermatid phenotype, suppressible | partially by Prtl99C^386.EGFP

(Eren-Ghiani et al., 2015)

Cad99C^248A has spermatozoon phenotype, suppressible | partially by Prtl99C^386.EGFP

(Eren-Ghiani et al., 2015)

Other

Statement

Reference

Cad99C[+]/Cad99C^248A, Prtl99C^MI10224 has spermatid phenotype

(Eren-Ghiani et al., 2015)

Cad99C^248A, Prtl99C^MI10224 has spermatozoon phenotype

(Eren-Ghiani et al., 2015)

Cad99C^248A, Prtl99C^MI10224 has spermatid phenotype

(Eren-Ghiani et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Prtl99C^{386.T:Avic\GFP-EGFP} partially rescues spermiogenesis in Cad99C^248A/Cad99C^248A mutants, with seminal vesicles full of motile sperm and more fertile males.

Prtl99C^MI10224,Cad99C^248A/Prtl99C^MI10224,Cad99C^248A double mutants have an elongated nucleus area (in spermatids during individualization as well as mature sperm) compared to controls.

Df(2L)FDD-0338164/Df(2L)FDD-0338164;Cad99C^248A/Cad99C^248A double mutant males are sterile and have longer spermatid nuclei than those seen in Cad99C^248A/Cad99C^248A mutants.

(Eren-Ghiani et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67200

Cad99C^248A/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cad99C^248A

(Eren-Ghiani et al., 2015, Schlichting et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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