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General Information
Symbol
Hsap\TARDBPUAS.cEa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Elden
FlyBase ID
FBal0270163
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-TDP-43
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Hsap\TARDBP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is ameliorated by Atx2X1
is exacerbated by Atx2EP3145
is ameliorated by PEKGL00030
is ameliorated by pAbpJF03104
is exacerbated by pAbpUAS.cSa
is ameliorated by Rox8HMS00472
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Hsap\TARDBPScer\UAS.cEa under the control of Scer\GAL4elav.Switch.PO show markedly reduced climbing ability compared to normal flies aged to 14 days when flies are raised with RU486.

Flies expressing Hsap\TARDBPScer\UAS.cEa under the control of Scer\GAL4elav.PLu exhibit reduced adult lifespan and reduced climbing ability. The reduced climbing activity is dramatically mitigated when the flies are fed the PERK inhibitor compound GSK2606415.

Directing expression of Hsap\TARDBPScer\UAS.cEa in the eye via Scer\GAL4GMR.PF causes a progressive, age-dependent degeneration of the retina structure.

Expression of Hsap\TARDBPScer\UAS.cEa in the nervous system under the control of Scer\GAL4elav-C155 induces markedly shortened lifespan.

Expression of Hsap\TARDBPScer\UAS.cEa in motor neurons under the control of Scer\GAL4D42 leads to a progressive loss of motility.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Rox8HMS00472 greatly suppresses the reduction in climbing ability seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4elav.Switch.PO and the flies are raised with RU486.

Expression of PEKGL00030 greatly suppresses the reduction in climbing ability seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4elav.Switch.PO and the flies are raised with RU486.

Expression of PPP1R15HMS00811 enhances the reduction in climbing ability seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4elav.Switch.PO when flies are raised with RU486. The flies almost entirely lose their motility.

Expression of Hsap\ATXN232Q.Scer\UAS enhances the eye phenotype seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4GMR.PU. The reduction in lifespan induced by the expression of Hsap\TARDBPScer\UAS.cEa (under the control of Scer\GAL4elav.PLu) and climbing defects (under the control of Scer\GAL4elav.Switch.PO and raised with RU486) are also enhanced.

Expression of Hsap\ATXN232Q.ΔLSM.Scer\UAS.T:Hsap\MYC enhances the eye phenotype seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4GMR.PU.

Expression of Hsap\ATXN232Q.ΔPAM2.Scer\UAS.T:Hsap\MYC does not enhance the eye phenotype seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4GMR.PU. The reduction in lifespan induced by the expression of Hsap\TARDBPScer\UAS.cEa (under the control of Scer\GAL4elav.PLu) and climbing defects (under the control of Scer\GAL4elav.Switch.PO and raised with RU486) are also not enhanced.

Expression of pAbpScer\UAS.cSa enhances the eye phenotype seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4GMR.PU.

Expression of pAbpJF03104 suppresses the reduction in climbing ability seen when Hsap\TARDBPScer\UAS.cEa is expressed under the control of Scer\GAL4elav.Switch.PO when flies are raised with RU486.

Overexpression of Atx2EP3145 via Scer\GAL4GMR.PF enhances the toxicity of Hsap\TARDBPScer\UAS.cEa in a markedly dose-dependent manner resulting in more severe retinal degeneration.

Overexpression of Atx2EP3145 via Scer\GAL4elav-C155 enhances the toxicity of Hsap\TARDBPScer\UAS.cEa in a markedly dose-dependent manner resulting in a further reduction of lifespan.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\TARDBPScer\UAS.cEa
Hsap\TARDBPUAS.cEa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Elden
Secondary FlyBase IDs
    References (5)